Recombinant Rat FGF-21, 大鼠蛋白FGF-21

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产品描述：

产品名称：Recombinant Rat FGF-21

氨基酸序列: AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS

参考文献: 1. Nishimura T, Nakatake Y, Konishi M, et al. 2000. Biochim Biophys Acta. 1492:203-6.<br/>2. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.<br/>3. Kurosu H, Choi M, Ogawa Y, et al. 2007. J Biol Chem. 282:26687-95

Accession: <a href="http://www.uniprot.org/uniprot/Q8VI80" target="_blank">Q8VI80</a>

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/170580" target="_blank">170580</a>

种属: Rat

纯度: > 95 % by SDS-PAGE and HPLC analyses.

来源: Escherichia coli.

内毒素水平: Less than 1 EU/μg of rRtFGF-21 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10³ IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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