Recombinant Rat FGF-18, 大鼠蛋白FGF-18

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产品描述：

产品名称：Recombinant Rat FGF-18

氨基酸序列: EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQTELQK PFKYTTVTKR SRRIRPTHPG

参考文献: 1. Itoh N, Ornitz DM. 2004. Trends Genet. 20:563-9.<br/>2. Itoh N. 2007. Biol Pharm Bull. 30:1819-25.<br/>3. Liu Z, Xu J, Colvin JS, et al. 2002. Genes Dev. 16:859-69.<br/>4. Ohbayashi N, Shibayama M, Kurotaki Y, et al. 2002. Genes Dev. 16:870-9.<br/>5. Usui H, Shibayama M, Ohbayashi N, et al. 2004. Biochem Biophys Res Commun. 322:887-92.<br/>6. Ellsworth JL, Berry J, Bukowski T, et al. 2002. Osteoarthritis Cartilage. 10:308-20.<br/>7. Moore EE, Bendele AM, Thompson DL, et al. 2005. Osteoarthritis Cartilage. 13:623-31.

Accession: <a href="http://www.uniprot.org/uniprot/O88182" target="_blank">O88182</a>

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/29369" target="_blank">29369</a>

种属: Rat

纯度: > 95 % by SDS-PAGE and HPLC analyses.

来源: Escherichia coli.

内毒素水平: Less than 1 EU/μg of rRtFGF-18 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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