Recombinant Mouse EGF, 小鼠蛋白EGF

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产品描述：

产品名称：Recombinant Mouse EGF

氨基酸序列: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

参考文献: 1. Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6.<br/>2. Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-62.<br/>3. Yang H, Sun X, Wang Z, et al. 2003. J Membr Biol, 194: 47-58.<br/>4. Cohen S. 2008. J Biol Chem, 283: 33793-7.

Accession: <a href="http://www.uniprot.org/uniprot/P01132" target="_blank">P01132</a>

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/13645" target="_blank">13645</a>

种属: Murine

纯度: > 97 % by SDS-PAGE and HPLC analyses.

来源: Escherichia coli.

内毒素水平: Less than 1 EU/μg of rMuEGF as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

形态: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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