eIF4A3-IN-2 is a highly selective and noncompetitive eukaryotic initiation factor 4A-3 (eIF4A3) inhibitor with an IC50 of 110 nM.
溶解性:In Vitro: DMSO : 100 mg/mL (165.92 mM; Need ultrasonic)
体外研究:
eIF4A3-IN-2 (compound 2) binds to the allosteric region in eIF4A3 and inhibits in vitro ATPase, helicase, and cellular nonsense-mediated RNA decay (NMD) activities.Apoptosis AnalysisCell Line:HEK293T cells (transfected with the NMD reporter) luciferase assayConcentration:0.3, 1, 3, or 10 μMIncubation Time:3 or 6 hResult:eIF4A3-IN-2 induces an approximately 3.2-fold increase in luciferase activity, indicating that NMD is inhibited by compound 2.