（一作视频讲解）J Agr Food Chem：湖南农大 | 低氮提升油菜氮利用率新机制

本文一作吴智敏文章解读

期刊：JOURNAL OF AGRICULTURALAND FOOD CHEMISTRY

主题：低氮提升油菜氮利用率新机制

标题：Low Nitrogen Enhances Nitrogen Use Efficiency by Triggering NO3– Uptake and Its Long-Distance Translocation

影响因子：3.412

检测指标：NO3-、H+

作者：湖南农业大学张振华、吴智敏

英文摘要

Nitrogen is essential for plant growth andcrop productivity; however, nitrogen use e?ciency (NUE) decreases withincreasing N supply, resulting in a waste of resources. Molecular mechanismsunderlying low-nitrogen (LN)-mediated enhancement of NUE are not clear.

We used high-NUE Brassica napus genotype H(Xiangyou 15), low-NUE B. napus genotype L (814), and Arabidopsis mutant aux1to elucidate the mechanism underlying the changes in NUE under di?erent ratesof N fertilizer application. NUE of B. napus increased under LN, which enhancedN uptake ability by regulating root system architecture and plasma membraneH+-ATPase activity; AUX1 was involved in this process.

Additionally, BnNRT1.5 was upregulated andBnNRT1.8 was downregulated under LN, whereby more N was transferred to theshoot through enhanced N transport. Observed changes in photosynthesis under LNwere associated with N assimilation e?ciency. Our study provides new insightsinto the mechanisms of plant adaptation to the environment.

中文摘要（谷歌机翻）

氮对植物生长和作物生产力至关重要；然而，随着氮供应的增加，氮利用效率（NUE）降低，导致资源浪费。低氮（LN）介导的NUE增强的分子机制尚不清楚。

我们使用高NUE甘蓝型油菜基因型H（香优15），低NUE B.napus基因型L（814）和拟南芥突变体aux1来阐明在不同氮肥施用量下NUE变化的机制。甘蓝型油菜NUE在LN下增加，通过调节根系结构和质膜H+ -ATPase活性提高N吸收能力; AUX1参与了这个过程。

另外，BnNRT1.5被上调并且BnNRT1.8在LN下被下调，由此通过增强的N转运将更多的N转移到芽。观察到的LN下光合作用的变化与N同化效率有关。我们的研究为植物适应环境的机制提供了新的见解。

Gas exchange, chlorophyll content, and photosynthetic N use in two contrasting B. napus genotypes for N use e?ciency under LN (1 mM NO3? N, black bars) or CK (15 mM NO3? N, white bars) conditions: (a) photosynthetic rate, (b) SPAD, (c) transpiration rate, and (d) photosynthetic N use e?ciency (PNUE). The bar height represents the mean, and error bars indicate standard deviation (n = 6). Di?erent letters on bars indicate signi?cant di?erences based on Tukey’s HSD (p < 0.05). The statistical analysis was performed on di?erent treatments within genotypes.

文章链接：

https://pubs.acs.org/doi/10.1021/acs.jafc.9b02491

本课题组往期NMT文章：

· V-ATPase and V-PPase at the Tonoplast Affect NO3- Content in Brassica napus by Controlling Distribution of NO3- Between the Cytoplasm and Vacuole

· Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus

· NRT1.1-related NH4+ toxicity is associated with balance between NH4+ uptake and assimilation

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