在本申请说明中，使用J-1500 CD光谱仪和ATS-530自动滴定仪在近紫外区域测量了溶菌酶与其抑制剂N-乙酰基-（D+）-葡糖胺（NAG）之间的相互作用。 关键词：J-1500，圆二色性，近紫外，侧链残留物，芳烃，ATS-530，自动滴定仪，生物化学

本申请说明证明了毛细管池和护套用于核糖核酸酶a的热倾斜研究。 关键词：圆二色性，毛细管池，微量分析，热变性，核糖核酸酶A，变性蛋白分析软件，变性温度，J-1500

The Stopped Flow method is used to analyze reactions occurred during the duration of several milliseconds and several seconds meanwhile the CD measurement provides valuable information regarding the protein’s secondary structures and environment of aromatic amino acids. Therefore, CD measurement with Stoppedflow method provides one of the best techniques in analyzing the protein’s Unfolding and Refolding process. Concanavalin A (derived from Jack bean) in natural state has abundant β-sheet structures, however, it is known that its structure changes into the one with rich α-helix under trifluoroethanol (TFE) and its Unfolding process has been reported also. In this application, the model SFS-492 High-Speed Stopped-flow System was used to measure the Unfoldin

Introduction The multivariate SSE program can be used to analyze sets of CD data. A large number of samples can be automatically measured and efficiently analyzed using the high-throughput circular dichroism (HDX) system and multivariate SSE program. Keywords: Secondary structure estimation, Multiple samples, HTCD, HDX Sequence Optical constants are automatically calculated in the multivariate SSE program after imputing parameters

CD spectrum and fluorescence spectrum are used in a complementary manner in the structural analysis of the proteins. The thermodynamic parameters of the protein obtained by the measurement with variable temperature provide the important knowledge for the thermal stability of the protein. The Temperature/Wavelength Scan program for JASCO CD spectrometers allows to obtain the data of CD with the temperature change for the protein and DNA required to calculate the denaturation temperature (Tm), enthalpy change (DH), and entropy change (DS). In addition such program also enables temperature interval measurements of CD and fluorescence spectra. The obtained data can be processed on the [Spectrum Analysis] and [Interval Analysis] program, which also enables to show the 3D representation. The secondary structure analysis and the calculation of thermodynamic parameters of the protein can be implemented by using optional [Thermal Denaturation Analysis] and [Protein SSE] program. This report introduces the measurement of Lysozyme (0.03 mg/mL) using Temperature/Wavelength Scan program as an example. Key words: CD spectra measurement, Fluorescence spectra measurement, Melting temperature, Proteins

Amphetamines are controlled substances which are potent stimulants that induce feelings of strength and energy while decreasing the need to sleep or eat. The majority of illegal amphetamine products are racemic; consequently, both isomers will appear in the biological fluids unchanged. In comparison the break-down products formed by the metabolism of most pharmaceutical drugs show the appearance of pure amphetamine enantiomers. Circular dichroism is a form of light absorption spectroscopy that measures the difference in absorbance of right- and leftcircularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. Since different enantiomers absorb different portions of the light, it provides and excellent means for enantiomer detection. In this application CD will be used to study the isomers of amphetamine and methamphetamine. Introduction Amphetamines are synthetic drugs that stimulate the

Introduction CD spectra provide information on the secondary structure of proteins and the environment of aromatic side chains. Therefore, CD measurement using a stopped-flow system is considered as one of the best methods for analyzing the unfolding and refolding of proteins. The existence of an intermediate between denaturated state and natural state during the refolding of proteins has been reported. The CD stopped-flow method is used for examining this refolding process. In this report the refolding process of cytochrome c (cyt c) measured using a SFS-492 stopped-flow system will be explained. Keywords: Stopped-flow, Circular Dichroism, Refolding Sample Preparation Aqueous solution of Cytochrome c denaturated by guanidine hydrochloride (GuHCl) was diluted with 0.1 M acetic acid buffer solution (1:9). The refolding process was observed at 222 nm for the secondary

Proteins, biological polymers composed of amino acids, have primary structures indicating the sequence of amino acids and higher three-dimensional order structures as well. The structure of proteins defines their biological functionality and CD spectroscopy is commonly used in the study of proteins and oligopeptide applications. Using secondary structure estimation (SSE) software, the helix, sheet and random coil contents of unknown proteins can be quantitatively estimated from CD spectra. SSE software has been applied mainly in academic or research studies of protein structure. JASCO now offers the new SSE Program based on the PLS/PCR method of multivariate analysis, to cope with expanding usage of protein studies across a wide range of fields, su

The IMV-4000 enables infrared imaging by a figure of two or more faster than using a conventional single element detector. A technique that quickly analyzes the results is also desirable. JASCO has proposed an analysis of imaging data with a variety of multivariate analysis techniques to accelerate the processing of infrared imaging data. For example, we have made it possible to easily create a visualization of the distribution map for the various components of multilayer film by analyzing imaging data using principle component analysis (PCA). We have also created a model that performs secondary structure estimation (SSE) on proteins based on IR spectra by principal components regression (PCR) and