圆二色谱法（CD）和荧光光谱法都是研究溶液状态蛋白质构象变化的简单而灵敏的技术。CD光谱测量左旋和右旋圆偏振光吸收的差异。本征荧光光谱法是研究蛋白质结构的一种补充技术。传统上，CD和荧光光谱是使用两种不同的仪器获得的。为了应对这些挑战，我们开发了一种CD和荧光光谱的自动测量系统。该系统由J-1500 CD光谱仪和HTCD-Plus自动CD测量单元以及荧光单色仪组成。使用该系统，我们通过改变尿素浓度和pH值，对曲妥珠单抗（赫赛汀）和利妥昔单抗（RIABNITM）进行了全面的稳定性评估。我们认为，该新系统对候选治疗性抗体的早期筛选特别有用。

在本申请说明中，来源于牛胸腺的血红蛋白和DNA的CD光谱是使用J-1500 CD光谱仪的微取样盘测量的。使用3µL样品体积进行测量，以证明J-1500在微样品池中具有小样品体积的灵敏度。 关键词：微量分析，微量取样盘，微样品池，圆二色性，血红蛋白，DNA，牛胸腺，二级结构，J-1500

圆二色性（CD）是一种在均匀条件下估计低浓度核酸三级结构的有效技术。本申请说明报告了使用高通量CD（HTCD）测量系统和主成分分析（PCA）对G-四链体（G4）适体的三级结构评估。 关键词：核酸医学、生物传感器、DNA适体、G-四链体、G4适体、圆二色性、高通量CD测量系统、HTCD、主成分分析、PCA

本应用说明强调了频谱QC测试程序的特点。 关键词：圆二色性，JWSQC-530，制药，生物化学，食品科学，可比性

本申请说明证明了使用HTCD系统的0.2mm路径长度样品池的测量再现性。关键词：高通量圆二色性（HTCD），制药，生物化学

当手性化合物用非偏振光激发时，可以测量左旋和右旋圆偏振光发射强度的差异。这种现象被称为圆偏振发光（CPL）。圆二色性提供了手性分子基态的信息，而CPL光谱则探测手性分子的激发态。 本申请说明演示了使用两种互补技术测量和分析樟脑醌：圆二色性和圆偏振发光。 关键词：圆偏振发光，圆二色性，CD-0031，材料

绿色荧光蛋白（GFP）是一种在暴露于紫外线时发出绿色荧光的蛋白质，并且已被证明具有手性性质。本申请说明说明了分别用CPL-300和J-1500获得的GFP的CPL和CD光谱。 关键词：J-1500，圆二色性，CPL-300，圆偏振发光，蛋白质结构，荧光，生物化学

本申请说明说明了使用J-1500 CD光谱仪、FMO-522发射单色仪附件和温度/波长扫描测量程序同时获得的溶菌酶的温度相关CD和荧光测量结果。 关键词：J-1500，FMO-522发射单色仪，圆二色性，荧光，二级结构，三级结构，热稳定性，生物化学

CD光谱是一种必不可少的对蛋白质结构分析和核磁共振一样或x射线晶体学测量方法。体积小或低浓度样品测量通用请求最近在这个市场。Jasco开发了新技术和应用程序以满足最终smallvolume或低浓度样品测量条件。本文,我们想展示用微抽样磁盘(MSD)测量一滴血CD光谱的结果。关键词:测量一滴血、蛋白质、圆二色性

CD光谱测量可用于有机化合物的光谱测量，本文中，我们将展示几个关于有机化合物的CD光谱数据。关键词:测量一滴血,圆形，二色性,有机化合物,金属络合物。

The Stopped Flow method is used to analyze reactions occurred during the duration of several milliseconds and several seconds meanwhile the CD measurement provides valuable information regarding the protein’s secondary structures and environment of aromatic amino acids. Therefore, CD measurement with Stoppedflow method provides one of the best techniques in analyzing the protein’s Unfolding and Refolding process. Concanavalin A (derived from Jack bean) in natural state has abundant β-sheet structures, however, it is known that its structure changes into the one with rich α-helix under trifluoroethanol (TFE) and its Unfolding process has been reported also. In this application, the model SFS-492 High-Speed Stopped-flow System was used to measure the Unfoldin

Introduction The multivariate SSE program can be used to analyze sets of CD data. A large number of samples can be automatically measured and efficiently analyzed using the high-throughput circular dichroism (HDX) system and multivariate SSE program. Keywords: Secondary structure estimation, Multiple samples, HTCD, HDX Sequence Optical constants are automatically calculated in the multivariate SSE program after imputing parameters

Amphetamines are controlled substances which are potent stimulants that induce feelings of strength and energy while decreasing the need to sleep or eat. The majority of illegal amphetamine products are racemic; consequently, both isomers will appear in the biological fluids unchanged. In comparison the break-down products formed by the metabolism of most pharmaceutical drugs show the appearance of pure amphetamine enantiomers. Circular dichroism is a form of light absorption spectroscopy that measures the difference in absorbance of right- and leftcircularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. Since different enantiomers absorb different portions of the light, it provides and excellent means for enantiomer detection. In this application CD will be used to study the isomers of amphetamine and methamphetamine.

CD spectrum and fluorescence spectrum are used in a complementary manner in the structural analysis of the proteins. The thermodynamic parameters of the protein obtained by the measurement with variable temperature provide the important knowledge for the thermal stability of the protein. The Temperature/Wavelength Scan program for JASCO CD spectrometers allows to obtain the data of CD with the temperature change for the protein and DNA required to calculate the denaturation temperature (Tm), enthalpy change (DH), and entropy change (DS). In addition such program also enables temperature interval measurements of CD and fluorescence spectra. The obtained data can be processed on the [Spectrum Analysis] and [Interval Analysis] program, which also enables to show the 3D representation. The secondary structure analysis and the calculation of thermodynamic parameters of the protein can be implemented by using optional [Thermal Denaturation Analysis] and [Protein SSE] program. This report introduces the measurement of Lysozyme (0.03 mg/mL) using Temperature/Wavelength Scan program as an example. Key words: CD spectra measurement, Fluorescence spectra measurement, Melting temperature, Proteins

Amphetamines are controlled substances which are potent stimulants that induce feelings of strength and energy while decreasing the need to sleep or eat. The majority of illegal amphetamine products are racemic; consequently, both isomers will appear in the biological fluids unchanged. In comparison the break-down products formed by the metabolism of most pharmaceutical drugs show the appearance of pure amphetamine enantiomers. Circular dichroism is a form of light absorption spectroscopy that measures the difference in absorbance of right- and leftcircularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. Since different enantiomers absorb different portions of the light, it provides and excellent means for enantiomer detection. In this application CD will be used to study the isomers of amphetamine and methamphetamine. Introduction Amphetamines are synthetic drugs that stimulate the

Introduction CD spectra provide information on the secondary structure of proteins and the environment of aromatic side chains. Therefore, CD measurement using a stopped-flow system is considered as one of the best methods for analyzing the unfolding and refolding of proteins. The existence of an intermediate between denaturated state and natural state during the refolding of proteins has been reported. The CD stopped-flow method is used for examining this refolding process. In this report the refolding process of cytochrome c (cyt c) measured using a SFS-492 stopped-flow system will be explained. Keywords: Stopped-flow, Circular Dichroism, Refolding Sample Preparation Aqueous solution of Cytochrome c denaturated by guanidine hydrochloride (GuHCl) was diluted with 0.1 M acetic acid buffer solution (1:9). The refolding process was observed at 222 nm for the secondary

Proteins, biological polymers composed of amino acids, have primary structures indicating the sequence of amino acids and higher three-dimensional order structures as well. The structure of proteins defines their biological functionality and CD spectroscopy is commonly used in the study of proteins and oligopeptide applications. Using secondary structure estimation (SSE) software, the helix, sheet and random coil contents of unknown proteins can be quantitatively estimated from CD spectra. SSE software has been applied mainly in academic or research studies of protein structure. JASCO now offers the new SSE Program based on the PLS/PCR method of multivariate analysis, to cope with expanding usage of protein studies across a wide range of fields, su

Carbon nanotubes are unique nanostructures with remarkable electronic and mechanical properties. Nanotubes have been considered prototypes for a one-dimensional quantum wire. As other useful properties have been discovered, particularly strength, interest has grown in potential applications,

The majority of printing problems are primarily caused by clogged print heads or nozzles, preventing the ink from reaching the paper. Each year billions

The use of Raman spectroscopy for routine quantitative analysis is desirable due to the simple sample handling requirements for sample analysis. Due to the many variables associated with Raman spectroscopy, specifically,

In recent years, the gemstone market has been flooded with stones of questionable origin. Frequently, even thorough analysis by a qualified jeweler cannot unequivocally reveal whether

Analysis Example by Vacuum Model FT/IR (1) Measurement of Silicon Wafer (1/1) No. 220TR0164-E Due to the high refractive index of silicon wafer samples, there is a correspondingly high reflectance from the sample. If the sample is placed directly in the sample compartment, vertical to the incident source beam, the reflection of the source returns to the interferometer, is reflected by the beamsplitter and then illuminates the sample again. As a result, the sample pathlength of the sample measurement is different from the background measurement and excessive noise is observed due to the atmospheric water vapor, even with a purged instrument. While it is possible to adjust the angle of the sample with respect to the incident source illumination, using an evacuated FT-IR instrument allows measurement of the sample vertical to the incident illumination without

Figure 1: ATR-500/Mi?ATR crystal plate (Upper right: Prism and liquid cell) Characterized by wavelengths longer than the mid-infrared region, the far-infrared region is also referred to as the terahertz region. In recent years, this region has been used to evaluate crystal polymorphs for pharmaceuticals and semiconductor device materials, as well as for archaeological research applications, including the study of inorganic pigments. Light in the far-infrared region (terahertz waves) corresponds to hydrogen bonding and Van der Waals forces believed to hold the key to an understanding of the functional

Introduction Near-Infrared (NIR) spectroscopy has unique capabilities as compared to mid-infrared analysis which can often require extensive sample preparation to obtain an identifiable spectrum. As such, NIR can be useful for the non-destructive analysis of a specific sample area or provide an average of a larger sample area, depending upon the required analysis. In recent years, NIR spectroscopy has been widely used for the examination of biological samples and quality control/analysis of food and medical products. The diffuse reflectance technique is ideal for this method due to extremely simple sample handling such that rapid identification of illegal drugs, such as MDMA, can be accomplished by using a search data library created using NIR diffuse reflectance.

The IMV-4000 enables infrared imaging by a figure of two or more faster than using a conventional single element detector. A technique that quickly analyzes the results is also desirable. JASCO has proposed an analysis of imaging data with a variety of multivariate analysis techniques to accelerate the processing of infrared imaging data. For example, we have made it possible to easily create a visualization of the distribution map for the various components of multilayer film by analyzing imaging data using principle component analysis (PCA). We have also created a model that performs secondary structure estimation (SSE) on proteins based on IR spectra by principal components regression (PCR) and

FT/IR-4000, 6000 series?? IRT-5000/7000 system?? * O2/N2 concentration measurement inside of Si wafer * Hydrogen??terminated evaluation on Si wafers * Quantitative/ Qualitative measurement of highly polymerized compounds ?? * Dynamic analysis of liquid crystal materials ?? * Reflectance of solar radiation on glass?? * Gas phase analysis ??

Analysis Example by Vacuum Model FT/IR (1) Measurement of Silicon Wafer (1/1) No. 220TR0164-E Due to the high refractive index of silicon wafer samples, there is a correspondingly high reflectance from the sample. If the sample is placed directly in the sample compartment, vertical to the incident source beam, the reflection of the source returns to the interferometer, is reflected by the beamsplitter and then illuminates the sample again. As a result, the sample pathlength of the sample measurement is different from the background measurement and excessive noise is observed due to the atmospheric water vapor, even with a purged instrument. While it is possible to adjust the angle of the sample with respect to the incident source illumination, using an evacuated FT-IR instrument allows measurement of the sample vertical to the incident illumination without

Gas Analysis System using Full Vacuum Type FT/IR (Trace amount of H2O in N2 gas) (1/2) No. 230TR0187-E The analysis of low concentration gases using long pathlength gas cells has been reported previously, however, it can be difficult to accurately quantitate gases at low concentrations when the absorption peaks of the target gas overlap with the peaks of atmospheric water vapor or, when the target gas itself is H2O or CO2, which are presnet in the atmosphere. This is because even with a vacuum FT-IR instrument, it was not possible to evacuate the sample chamber with the long pathlength cell present. The full vacuum gas analysis system introduced in this application note has a special gas cell integrated into the sample chamber, allowing the entire light path to remain under full vacuum with the gas cell in place. This full vacuum model makes it possible to quantitate concentrations of H2O even at the 0.1 ppm level. For the measurement, there are separate vacuum lines for evacuation of the FT-IR instrument and the gas cell, with independent evacuation capability for the separate components. Adding a vacuum gauge to the gas cell makes it possible to control extremely

The use of near-infrared (NIR) spectroscopy in the pharmaceutical industry has grown rapidly in the past decade. The IMV-4000 Infrared Multichannel Viewer integrated with a NIR capable FT/IR- 4000 or FT/IR-6000 instrument enables NIR Imaging of pharmaceutical tablets, a valuable tool in the estimation of tablet properties, offering measurement data with high specificity and sensitivity. The obtained data can be exported into the Principal Component Analysis (PCA) software for the estimation of tablet characteristics. Instrument: FTIR-6200 with IMV-4000 CaF2 beam splitter, NIR source, etc

Laminates are combinations of polymers that combine the benefits and properties of the various polymers used in the laminate to create a better material. Polymer laminates have a multitude of uses including packaging for food and other products. Laminates act as moisture and/or oxygen barriers and as such are commonly used to protect foods and chemicals. Analysis of laminates is often required for quality control and to determine laminate adhesion problems.