大脑蛋白2抗体elisa实验

大脑蛋白2抗体英文名称  Anti-Brn-2/POU3F2 

中文名称  大脑蛋白2抗体 

别    名  Brain 2; Brain specific homeobox/POU domain protein 2; Brain-2; Brain-specific homeobox/POU domain protein 2; BRN 2; Brn-2; BRN2; N Oct 3; N OCT 3 Gene; Nervous system specific octamer binding transcription factor; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; OCT7; Octamer Binding Transcription Factor 7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF 7; OTF-7; OTF7; PO3F2_HUMAN; POU class 3 homeobox 2; POU domain class 3 transcription factor 2; POU domain, class 3, transcription factor 2; Pou3f2; POUF 3; POUF3; Protein Brn 2. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg   

大脑蛋白2抗体抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit   

产品类型  一抗    

研究领域  细胞生物 免疫学 神经生物学 表观遗传学  

蛋白分子量  predicted molecular weight: 49kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human Brn-2 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-F=1:100-500  IF=1:50-200 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU domain proteins. Expressed during the development of the forebrain and coexpressed in most layer II-V cortical neurons, Brn-1 and Brn-2 appear to critically control the initiation of radial migration of cortical neurons. Brn-2 is thought to be involved in smooth muscle cell development and differentiation. Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. Brn-3a has two functional transactivating domains, one at the amino terminus and one at the carboxy terminus. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor.

Function : Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.

Subunit : Interacts with PQBP1.

大脑蛋白2抗体Subcellular Location : Nucleus.

Tissue Specificity : Expressed specifically in the neuroectodermal cell lineage.

Similarity : Belongs to the POU transcription factor family. Class-3 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Database links : UniProtKB/Swiss-Prot: P20265.4