丁酰胆碱酯酶抗体(N端)elisa实验

丁酰胆碱酯酶抗体(N端)英文名称  Anti-BCHE/Butyrylcholinesterase (NT) 

中文名称  丁酰胆碱酯酶抗体(N端) 

别    名  Cholinesterase; Acylcholine acylhydrolase; Choline esterase II; CHE1; Butyrylcholine esterase; Pseudocholinesterase; butyrylcholinesterase. CHLE_HUMAN. 

浓    度  1mg/1ml 

丁酰胆碱酯酶抗体(N端)规 格  0.1ml/100μg  0.2ml/200μg  

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Cow

产品类型  一抗    

研究领域  肿瘤 细胞生物 神经生物学 信号转导 Alzheimer's  

蛋白分子量  predicted molecular weight: 66kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human BCHE N-terminus 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008].

Function : Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

Subunit : Homotetramer; disulfide-linked. Dimer of dimers.

Subcellular Location : Secreted.

Tissue Specificity : Detected in blood plasma (at protein level). Present in most cells except erythrocytes.

DISEASE : Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]. BChE deficiency is a metabolic disorder 丁酰胆碱酯酶抗体(N端)characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait.

Similarity : Belongs to the type-B carboxylesterase/lipase family.

Database links : UniProtKB/Swiss-Prot: P06276.1