溴脱氧尿苷抗体elisa实验

溴脱氧尿苷抗体英文名称  Anti-BrdU 

中文名称  溴脱氧尿苷抗体 

别    名  Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg  1ml/1mg 

抗体来源  Rabbit  

溴脱氧尿苷抗体克隆类型  polyclonal 

交叉反应  BrdU  

产品类型  一抗  药物与化合物抗体   

研究领域  细胞生物 发育生物学 染色质和核信号 神经生物学 细胞凋亡 细胞周期蛋白 药物及化合物 细胞类型标志物  

蛋白分子量  predicted molecular weight: 0.3071kDa 

性    状  Lyophilized or Liquid 

免 疫 原  bromodeoxyuridine conjugated to KLH 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 0.01M PBS, pH 7.4 

产品应用   ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying 溴脱氧尿苷抗体proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985).

Subcellular Location : Nuclear.

BrdU（溴化脱氧尿嘧啶核苷）可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。此抗体可以与掺入到任何种属细胞中的BrdU反应，与碘脱氧尿嘧啶有交叉反应，标记掺有BrdU的S期细胞，主要用于研究各种不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。