程序性死亡配体2抗体elisa实验

程序性死亡配体2抗体英文名称  Anti-B7-DC/CD273/PDL2 

中文名称  程序性死亡配体2抗体 

别    名  B7 dendritic cell molecule; B7-DC; B7DC; bA574F11.2; Btdc; Butyrophilin B7 DC; Butyrophilin B7-DC; Butyrophilin B7DC; CD 273; CD273; CD273 antigen; MGC142238; MGC142240; PD 1 ligand 2; PD L2; PD-1 ligand 2; PD-L2; PD1 ligand 2; PD1L2_HUMAN; PDCD 1 ligand 2; PDCD1 ligand 2; PDCD1L2; Pdcd1lg2; PDL 2; PDL2; Programmed cell death 1 ligand 2; Programmed death ligand 2. 

浓    度  1mg/1ml 

程序性死亡配体2抗体规 格  0.1ml/100μg  0.2ml/200μg    

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Cow  

产品类型  一抗    

研究领域  细胞生物 免疫学  

蛋白分子量  predicted molecular weight: 29kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human B7-DC (41-85aa) 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 B7DC is a 33kDa member of the immunoglobulin receptor superfamily expressed on DC, liver and a small subset of macrophages as well as a few transformed cell lines. B7DC has been reported to be stimulatory on dendritic cells when cross linked and to inhibit T cell activation upon engaging the PD1 receptor. B7DC has also been reported to bind to an alternative receptor and to mediate T cell activation through non PD1 mediated interactions.

Function : Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).

Subunit : Interacts with PDCD1 (By similarity). 

Subcellular Location : Isoform 3: Secreted (Probable).

Isoform 2: Endomembrane system; Single-pass type I membrane protein.

Isoform 1: Cell membrane; Single-pass type I membrane protein.

程序性死亡配体2抗体Tissue Specificity : Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. 

Similarity : Belongs to the immunoglobulin superfamily. BTN/MOG family. 

Contains 1 Ig-like C2-type (immunoglobulin-like) domain. 

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Database links : UniProtKB/Swiss-Prot: Q9BQ51.2

结合受体PD-1（CD279），抑制T细胞增殖和细胞因子产生