膜相关环指蛋白2抗体

膜相关环指蛋白2抗体 

『别名』E3 ubiquitin protein ligase MARCH2; E3 ubiquitin-protein ligase MARCH2; EC 6.3.2.- antibody HSPC240; MARCH II; MARCH-II; march2; MARH2_HUMAN antibody Membrane associated RING CH protein II; Membrane associated ring finger (C3HC4) 2; Membrane associated RING finger protein 2; Membrane-associated RING finger protein 2; Membrane-associated RING-CH protein II; RING finger protein 172; RNF172. 

『浓度』膜相关环指蛋白2抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

膜相关环指蛋白2抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

膜相关环指蛋白2抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

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AA098Po21 基质金属蛋白酶10(MMP10)单克隆抗体 Monoclonal Antibody to Matrix Metalloproteinase 10 (MMP10)

AA098Ra21 基质金属蛋白酶10(MMP10)单克隆抗体 Monoclonal Antibody to Matrix Metalloproteinase 10 (MMP10)

AA097Hu22 基质金属蛋白酶1(MMP1)单克隆抗体 Monoclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

AA097Bo21 基质金属蛋白酶1(MMP1)单克隆抗体 Monoclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

AA097Eq21 基质金属蛋白酶1(MMP1)单克隆抗体 Monoclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

AA097Ov21 基质金属蛋白酶1(MMP1)单克隆抗体 Monoclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

AA097Po21 基质金属蛋白酶1(MMP1)单克隆抗体 Monoclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

AA097Ra21 基质金属蛋白酶1(MMP1)单克隆抗体 Monoclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

AA097Rb21 基质金属蛋白酶1(MMP1)单克隆抗体 Monoclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

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AB232Ra21 细胞外基质磷酸糖蛋白(MEPE)单克隆抗体 Monoclonal Antibody to Matrix Extracellular Phosphoglycoprotein (MEPE)

AH678Hu21 Matrin 3蛋白(MATR3)单克隆抗体 Monoclonal Antibody to Matrin 3 (MATR3)

AH665Hu21 母系胚胎亮氨酸拉链蛋白激酶(MELK)单克隆抗体 Monoclonal Antibody to Maternal Embryonic Leucine Zipper Kinase (MELK)

AH665Ra21 母系胚胎亮氨酸拉链蛋白激酶(MELK)单克隆抗体 Monoclonal Antibody to Maternal Embryonic Leucine Zipper Kinase (MELK)

AL709Hu21 MARCKS相关蛋白(MARCKSL1)单克隆抗体 Monoclonal Antibody to MARCKS Related Protein (MARCKSL1)

AS723Hu21 含MANSC域蛋白1(MANSC1)单克隆抗体 Monoclonal Antibody to MANSC Domain Containing Protein 1 (MANSC1)

AH694Hu21 甘露糖苷酶α2B类成员1(MAN2B1)单克隆抗体 Monoclonal Antibody to Mannosidase Alpha Class 2B Member 1 (MAN2B1)

AH689Hu21 甘露糖苷酶α1A类成员1(MAN1A1)单克隆抗体 Monoclonal Antibody to Mannosidase Alpha Class 1A Member 1 (MAN1A1)

AH700Hu21 甘露糖-6-磷酸受体(M6PR)单克隆抗体 Monoclonal Antibody to Mannose-6-Phosphate Receptor (M6PR)

AA733Hu22 甘露糖受体C2(MRC2)单克隆抗体 Monoclonal Antibody to Mannose Receptor C Type 2 (MRC2)

AT618Hu21 甘露糖受体C1样蛋白1(MRC1)单克隆抗体 Monoclonal Antibody to Mannose Receptor C Type 1 Like Protein 1(MRC1)

AB542Hu22 甘露糖受体C1(MRC1)单克隆抗体 Monoclonal Antibody to Mannose Receptor C Type 1 (MRC1)

AB542Ra21 甘露糖受体C1(MRC1)单克隆抗体 Monoclonal Antibody to Mannose Receptor C Type 1 (MRC1)

AH620Hu21 甘露糖磷酸异构酶(MPI)单克隆抗体 Monoclonal Antibody to Mannose Phosphate Isomerase (MPI)

AB480Hu22 甘露糖结合蛋白(MBL)单克隆抗体 Monoclonal Antibody to Mannose Binding Lectin (MBL)

AB480Ga21 甘露糖结合蛋白(MBL)单克隆抗体 Monoclonal Antibody to Mannose Binding Lectin (MBL)

AA859Hu22 甘露聚糖结合凝集素关联丝氨酸蛋白酶2(MASP2)单克隆抗体 Monoclonal Antibody to Mannose Associated Serine Protease 2 (MASP2)

AA859Hu21 甘露聚糖结合凝集素关联丝氨酸蛋白酶2(MASP2)单克隆抗体 Monoclonal Antibody to Mannose Associated Serine Protease 2 (MASP2)

AB895Hu22 甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)单克隆抗体 Monoclonal Antibody to Mannose Associated Serine Protease 1 (MASP1)

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AA253Hu22 麦芽糖酶糖化酶(MGA)单克隆抗体 Monoclonal Antibody to Maltase Glucoamylase, Intestinal (MGA)

AH669Hu21 NADP依赖性苹果酶2(ME2)单克隆抗体 Monoclonal Antibody to Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2)

AH672Hu21 苹果酸脱氢酶2(MDH2)单克隆抗体 Monoclonal Antibody to Malate Dehydrogenase 2 (MDH2)

AH673Hu21 苹果酸脱氢酶1(MDH1)单克隆抗体 Monoclonal Antibody to Malate Dehydrogenase 1 (MDH1)

AH673Ra21 苹果酸脱氢酶1(MDH1)单克隆抗体 Monoclonal Antibody to Malate Dehydrogenase 1 (MDH1)

AB764Hu21 主要穹窿蛋白(MVP)单克隆抗体 Monoclonal Antibody to Major Vault Protein (MVP)

AC167Hu21 Ⅱ类主要组织相容性复合体DRβ1(MHCDRβ1)单克隆抗体 Monoclonal Antibody to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1)

AC166Si21 Ⅱ类主要组织相容性复合体DRα(MHCDRα)单克隆抗体 Monoclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)

AC165Hu21 Ⅱ类主要组织相容性复合体DQβ1(MHCDQβ1)单克隆抗体 Monoclonal Antibody to Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1)

AB856Hu22 Ⅰ类主要组织相容性复合体G(MHCG)单克隆抗体 Monoclonal Antibody to Major Histocompatibility Complex Class I G (MHCG)

AB752Hu22 Ⅰ类主要组织相容性复合体E(MHCE)单克隆抗体 Monoclonal Antibody to Major Histocompatibility Complex Class I E (MHCE)

AC086Hu22 Ⅰ类主要组织相容性复合体C(MHCC)单克隆抗体 Monoclonal Antibody to Major Histocompatibility Complex Class I C (MHCC)

AC092Hu21 Ⅰ类主要组织相容性复合体B(MHCB)单克隆抗体 Monoclonal Antibody to Major Histocompatibility Complex Class I B (MHCB)

AC085Hu21 Ⅰ类主要组织相容性复合体A(MHCA)单克隆抗体 Monoclonal Antibody to Major Histocompatibility Complex Class I A (MHCA)

AB650Hu21 主要碱性蛋白(MBP)单克隆抗体 Monoclonal Antibody to Major Basic Protein (MBP)

AB121Hu21 巨噬细胞刺激蛋白(MSP)单克隆抗体 Monoclonal Antibody to Macrophage Stimulating Protein (MSP)

AB121Ra21 巨噬细胞刺激蛋白(MSP)单克隆抗体 Monoclonal Antibody to Macrophage Stimulating Protein (MSP)

AB591Hu22 巨噬细胞清道夫受体1(MSR1)单克隆抗体 Monoclonal Antibody to Macrophage Scavenger Receptor 1 (MSR1)

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AC091Hu21 巨噬细胞炎性蛋白4α(MIP4α)单克隆抗体 Monoclonal Antibody to Macrophage Inflammatory Protein 4 Alpha (MIP4a)

AA096Hu22 巨噬细胞炎性蛋白3β(MIP3β)单克隆抗体 Monoclonal Antibody to Macrophage Inflammatory Protein 3 Beta (MIP3b)

AA096Ca21 巨噬细胞炎性蛋白3β(MIP3β)单克隆抗体 Monoclonal Antibody to Macrophage Inflammatory Protein 3 Beta (MIP3b)

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AA095Hu22 膜相关环指蛋白2抗体巨噬细胞炎性蛋白3α(MIP3α)单克隆抗体 Monoclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)

AA095Ra21 巨噬细胞炎性蛋白3α(MIP3α)单克隆抗体 Monoclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)

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