膜相关环指蛋白1抗体

膜相关环指蛋白1抗体 

『别名』E3 ubiquitin protein ligase MARCH1; E3 ubiquitin-protein ligase MARCH1; MARCH 1; MARCH-I; March1; MARH1_HUMAN; Membrane associated RING CH protein I; Membrane associated ring finger (C3HC4) 1; Membrane associated RING finger protein 1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; RING finger protein 171; RNF171. 

『浓度』膜相关环指蛋白1抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

膜相关环指蛋白1抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

膜相关环指蛋白1抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

AB677Hu22黑素转铁蛋白(MFI2)单克隆抗体Monoclonal Antibody to Melanotransferrin (MFI2)

AH650Hu21黑素瘤抑制性活性蛋白1(MIA1)单克隆抗体Monoclonal Antibody to Melanoma Inhibitory Activity Protein 1 (MIA1)

AH650Ra21黑素瘤抑制性活性蛋白1(MIA1)单克隆抗体Monoclonal Antibody to Melanoma Inhibitory Activity Protein 1 (MIA1)

AB646Hu22黑素瘤衍生亮氨酸拉链额外核因子(MLZE)单克隆抗体Monoclonal Antibody to Melanoma Derived Leucine Zipper Extra Nuclear Factor (MLZE)

AB670Hu22黑素瘤细胞黏附分子(MCAM)单克隆抗体Monoclonal Antibody to Melanoma Cell Adhesion Molecule (MCAM)

AB330Hu22黑色素瘤关联ME20(ME20M)单克隆抗体Monoclonal Antibody to Melanoma Associated ME20 (ME20M)

AB134Hu22黑色素瘤关联硫酸软骨素蛋白聚糖(MCSP)单克隆抗体Monoclonal Antibody to Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP)

AF605Hu21黑素皮质激素5受体(MC5R)单克隆抗体Monoclonal Antibody to Melanocortin 5 Receptor (MC5R)

AC946Hu21黑色素聚集激素受体1(MCHR1)单克隆抗体Monoclonal Antibody to Melanin Concentrating Hormone Receptor 1 (MCHR1)

AG790Hu21Mdm2 p53结合蛋白同源物(MDM2)单克隆抗体Monoclonal Antibody to Mdm2 p53 Binding Protein Homolog (MDM2)

AP859Hu21基质重塑关联蛋白5(MXRA5)单克隆抗体Monoclonal Antibody to Matrix Remodelling Associated Protein 5 (MXRA5)

AA553Hu22基质金属蛋白酶9(MMP9)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

AA553Bo21基质金属蛋白酶9(MMP9)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

AA553Ca21基质金属蛋白酶9(MMP9)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

AA553Eq21基质金属蛋白酶9(MMP9)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

AA553Po21基质金属蛋白酶9(MMP9)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

AA553Ra21基质金属蛋白酶9(MMP9)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

AA553Rb21基质金属蛋白酶9(MMP9)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

AA103Hu22基质金属蛋白酶8(MMP8)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 8 (MMP8)

AA103Gu21基质金属蛋白酶8(MMP8)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 8 (MMP8)

AA103Ra21基质金属蛋白酶8(MMP8)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 8 (MMP8)

AA102Hu22基质金属蛋白酶7(MMP7)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 7 (MMP7)

AA102Bo21基质金属蛋白酶7(MMP7)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 7 (MMP7)

AA102Cp21基质金属蛋白酶7(MMP7)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 7 (MMP7)

AA102Eq21基质金属蛋白酶7(MMP7)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 7 (MMP7)

AA102Ra21基质金属蛋白酶7(MMP7)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 7 (MMP7)

AA101Hu22基质金属蛋白酶3(MMP3)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 3 (MMP3)

AA101Bo21基质金属蛋白酶3(MMP3)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 3 (MMP3)

AA101Ca21基质金属蛋白酶3(MMP3)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 3 (MMP3)

AA101Eq21基质金属蛋白酶3(MMP3)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 3 (MMP3)

AA101Po21基质金属蛋白酶3(MMP3)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 3 (MMP3)

AA101Ra21基质金属蛋白酶3(MMP3)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 3 (MMP3)

AA101Rb21基质金属蛋白酶3(MMP3)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 3 (MMP3)

AB999Hu22基质金属蛋白酶28(MMP28)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 28 (MMP28)

AB256Hu22基质金属蛋白酶26(MMP26)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 26 (MMP26)

AB927Hu22基质金属蛋白酶25(MMP25)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 25 (MMP25)

AA212Hu22基质金属蛋白酶23B(MMP23B)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 23B (MMP23B)

AA212Ra21基质金属蛋白酶23B(MMP23B)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 23B (MMP23B)

AA211Hu22基质金属蛋白酶23A(MMP23A)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 23A (MMP23A)

AA100Hu22基质金属蛋白酶2(MMP2)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2)

AA100Eq21基质金属蛋白酶2(MMP2)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2)

AA100Po21基质金属蛋白酶2(MMP2)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2)

AA100Ra21基质金属蛋白酶2(MMP2)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2)

AA100Rb21基质金属蛋白酶2(MMP2)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2)

AB853Hu22基质金属蛋白酶19(MMP19)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 19 (MMP19)

AB846Hu21基质金属蛋白酶17(MMP17)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 17 (MMP17)

AB533Hu22基质金属蛋白酶16(MMP16)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 16 (MMP16)

AA322Hu22基质金属蛋白酶15(MMP15)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 15 (MMP15)

AC056Hu22基质金属蛋白酶14(MMP14)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 14 (MMP14)

AA099Hu22基质金属蛋白酶13(MMP13)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 13 (MMP13)

AA099Bo21基质金属蛋白酶13(MMP13)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 13 (MMP13)

AA099Cp21膜相关环指蛋白1抗体基质金属蛋白酶13(MMP13)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 13 (MMP13)

AA099Eq21基质金属蛋白酶13(MMP13)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 13 (MMP13)

AA099Ga21基质金属蛋白酶13(MMP13)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 13 (MMP13)

AA099Po21基质金属蛋白酶13(MMP13)单克隆抗体Monoclonal Antibody to Matrix Metalloproteinase 13 (MMP13)