结核分枝杆菌抗体

结核分枝杆菌抗体 

『别名』Antigen 85 complex B; 30 kDa extracellular protein; Mycobacterium; Mycolyl transferase 85B; fbpB; Antigen 85-B; 85B; Ag85B; Extracellular alpha-antigen; Fibronectin-binding protein B; Rv1886c; MT1934; MTCY180.32. 

『浓度』结核分枝杆菌抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

结核分枝杆菌抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

结核分枝杆菌抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

AH676Hu21 MYC关联因子X(MAX)单克隆抗体 Monoclonal Antibody to MYC Associated Factor X (MAX)

AJ746Hu21 MutY同源物(MUTYH)单克隆抗体 Monoclonal Antibody to MutY Homolog (MUTYH)

AJ742Hu21 MutL同源物1(MLH1)单克隆抗体 Monoclonal Antibody to MutL Homolog 1 (MLH1)

AE270Hu21 骨骼肌特异性受体酪氨酸激酶(MUSK)单克隆抗体 Monoclonal Antibody to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)

AF593Hu21 多聚蛋白2(MMRN2)单克隆抗体 Monoclonal Antibody to Multimerin 2 (MMRN2)

AC622Hu22 多聚蛋白1(MMRN1)单克隆抗体 Monoclonal Antibody to Multimerin 1 (MMRN1)

AC622Ra21 多聚蛋白1(MMRN1)单克隆抗体 Monoclonal Antibody to Multimerin 1 (MMRN1)

AB068Hu22 多药耐药关联蛋白1(MRP1)单克隆抗体 Monoclonal Antibody to Multidrug Resistance Associated Protein 1 (MRP1)

AB068Ra21 多药耐药关联蛋白1(MRP1)单克隆抗体 Monoclonal Antibody to Multidrug Resistance Associated Protein 1 (MRP1)

AB521Hu22 黏膜地址素细胞黏附分子(MAdCAM1)单克隆抗体 Monoclonal Antibody to Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1)

AB573Hu21 粘膜关联上皮趋化因子(MEC)单克隆抗体 Monoclonal Antibody to Mucosae Associated Epithelia Chemokine (MEC)

AB808Hu22 粘蛋白7(MUC7)单克隆抗体 Monoclonal Antibody to Mucin 7, Secreted (MUC7)

AB489Hu22 粘蛋白6(MUC6)单克隆抗体 Monoclonal Antibody to Mucin 6 (MUC6)

AA684Hu22 粘蛋白5B(MUC5B)单克隆抗体 Monoclonal Antibody to Mucin 5 Subtype B (MUC5B)

AA756Hu22 粘蛋白5AC(MUC5AC)单克隆抗体 Monoclonal Antibody to Mucin 5 Subtype AC (MUC5AC)

AA756Hu21 粘蛋白5AC(MUC5AC)单克隆抗体 Monoclonal Antibody to Mucin 5 Subtype AC (MUC5AC)

AA756Ra21 粘蛋白5AC(MUC5AC)单克隆抗体 Monoclonal Antibody to Mucin 5 Subtype AC (MUC5AC)

AB034Hu22 粘蛋白4(MUC4)单克隆抗体 Monoclonal Antibody to Mucin 4 (MUC4)

AB031Hu22 粘蛋白3(MUC3)单克隆抗体 Monoclonal Antibody to Mucin 3 (MUC3)

AD184Hu21 粘蛋白21(MUC21)单克隆抗体 Monoclonal Antibody to Mucin 21 (MUC21)

AA995Hu22 粘蛋白20(MUC20)单克隆抗体 Monoclonal Antibody to Mucin 20 (MUC20)

AA705Hu22 粘蛋白2(MUC2)单克隆抗体 Monoclonal Antibody to Mucin 2 (MUC2)

AA705Ra21 粘蛋白2(MUC2)单克隆抗体 Monoclonal Antibody to Mucin 2 (MUC2)

AA403Hu22 粘蛋白17(MUC17)单克隆抗体 Monoclonal Antibody to Mucin 17 (MUC17)

AP861Hu21 粘蛋白13(MUC13)单克隆抗体 Monoclonal Antibody to Mucin 13, Cell Surface Associated (MUC13)

AP862Hu21 粘蛋白12(MUC12)单克隆抗体 Monoclonal Antibody to Mucin 12, Cell Surface Associated (MUC12)

AA413Hu22 粘蛋白1(MUC1)单克隆抗体 Monoclonal Antibody to Mucin 1 (MUC1)

AA413Bo22 粘蛋白1(MUC1)单克隆抗体 Monoclonal Antibody to Mucin 1 (MUC1)

AA413Ra21 粘蛋白1(MUC1)单克隆抗体 Monoclonal Antibody to Mucin 1 (MUC1)

AB097Hu22 运动因子相关蛋白1(MRP1)单克隆抗体 Monoclonal Antibody to Motility Related Protein (MRP1)

AA575Hu22 胃动素(MTL)单克隆抗体 Monoclonal Antibody to Motilin (MTL)

AA575Ra21 胃动素(MTL)单克隆抗体 Monoclonal Antibody to Motilin (MTL)

AA648Hu22 Smad同源物7(Smad7)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 7 (Smad7)

AA648Ra21 Smad同源物7(Smad7)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 7 (Smad7)

AB925Hu22 Smad同源物4(Smad4)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)

AC123Hu22 Smad同源物3(Smad3)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 3 (Smad3)

AC123Ra21 Smad同源物3(Smad3)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 3 (Smad3)

AC124Hu21 Smad同源物2(Smad2)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)

AA647Hu22 Smad同源物1(Smad1)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)

AA647Ra21 Smad同源物1(Smad1)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)

AB928Hu22 干扰素γ诱导单核因子(MIγ)单克隆抗体 Monoclonal Antibody to Monokine Induced By Interferon Gamma (MIg)

AB928Hu21 干扰素γ诱导单核因子(MIγ)单克隆抗体 Monoclonal Antibody to Monokine Induced By Interferon Gamma (MIg)

AB928Ra21 干扰素γ诱导单核因子(MIγ)单克隆抗体 Monoclonal Antibody to Monokine Induced By Interferon Gamma (MIg)

AB584Hu22 单核细胞巨噬细胞分化关联蛋白(MMA)单克隆抗体 Monoclonal Antibody to Monocyte To Macrophage Differentiation Associated Protein (MMA)

AA216Hu22 单核细胞趋化蛋白4(MCP4)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 4 (MCP4)

AA089Hu22 单核细胞趋化蛋白3(MCP3)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 3 (MCP3)

AA089Ra21 单核细胞趋化蛋白3(MCP3)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 3 (MCP3)

AA088Hu22 单核细胞趋化蛋白2(MCP2)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 2 (MCP2)

AA088Bo21 单核细胞趋化蛋白2(MCP2)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 2 (MCP2)

AA088Ca21 单核细胞趋化蛋白2(MCP2)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 2 (MCP2)

AA088Po21 单核细胞趋化蛋白2(MCP2)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 2 (MCP2)

AA087Hu22 单核细胞趋化蛋白1(MCP1)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 1 (MCP1)

AA087Rb21 单核细胞趋化蛋白1(MCP1)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 1 (MCP1)

AA087Ca21 单核细胞趋化蛋白1(MCP1)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 1 (MCP1)

AA087Po21 单核细胞趋化蛋白1(MCP1)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 1 (MCP1)

AA087Mi21 单核细胞趋化蛋白1(MCP1)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 1 (MCP1)

AA087Si21 单核细胞趋化蛋白1(MCP1)单克隆抗体 Monoclonal Antibody to Monocyte Chemotactic Protein 1 (MCP1)

AB954Ra21 单氨氧化酶A结核分枝杆菌抗体(MAOA)单克隆抗体 Monoclonal Antibody to Monoamine Oxidase A (MAOA)

AH629Hu21 单酰甘油-O-酰基转移酶2(MOGAT2)单克隆抗体 Monoclonal Antibody to Monoacylglycerol-O-acyltransferase 2 (MOGAT2)

AC642Hu22 膜突蛋白(MSN)单克隆抗体 Monoclonal Antibody to Moesin (MSN)

AR574Hu21 含MOCO硫化酶羧基端域蛋白1(MOSC1)单克隆抗体 Monoclonal Antibody to MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1)

AD558Ra21 丝裂原激活蛋白激酶激酶激酶6(MAP3K6)单克隆抗体 Monoclonal Antibody to Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6)