蛋白激酶MST抗体

蛋白激酶MST抗体 

『别名』 STK4; Krs2; Mammalian STE20 like protein kinase 1; Mammalian sterile 20 like 1; Serine/threonine protein kinase Krs 2; serine/threonine kinase 4; STE20-like kinase MST1; Stk4; MST-1; STE20-like kinase MST1; Serine/threonine-protein kinase Krs-2; serine/threonine kinase 4 variant. 

『浓度』蛋白激酶MST抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

蛋白激酶MST抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

蛋白激酶MST抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

AC838Hu21 Twist转录因子(TWIST)单克隆抗体 Monoclonal Antibody to Twist Transcription Factor (TWIST)

AM410Hu21 双解丝蛋白1(TWF1)单克隆抗体 Monoclonal Antibody to Twinfilin 1 (TWF1)

AC814Hu21 肿瘤易感基因101(TSG101)单克隆抗体 Monoclonal Antibody to Tumor Susceptibility Gene 101 (TSG101)

AG962Hu21 翻译调节肿瘤蛋白1(TPT1)单克隆抗体 Monoclonal Antibody to Tumor Protein, Translationally Controlled 1 (TPT1)

AC805Hu22 肿廇蛋白P63(TP63)单克隆抗体 Monoclonal Antibody to Tumor Protein P63 (TP63)

AH009Hu21 肿瘤蛋白p53结合蛋白1(TP53BP1)单克隆抗体 Monoclonal Antibody to Tumor Protein p53 Binding Protein 1 (TP53BP1)

AA928Hu22 肿瘤蛋白p53(TP53)单克隆抗体 Monoclonal Antibody to Tumor Protein p53 (TP53)

AA928Ra21 肿瘤蛋白p53(TP53)单克隆抗体 Monoclonal Antibody to Tumor Protein p53 (TP53)

AA139Hu22 肿瘤坏死因子相关凋亡诱导配体(TRAIL)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)

AA139Ca21 肿瘤坏死因子相关凋亡诱导配体(TRAIL)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)

AA139Ra21 肿瘤坏死因子相关凋亡诱导配体(TRAIL)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)

AB043Hu21 肿瘤坏死因子受体超家族成员7(TNFRSF7)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)

AB249Ra21 肿瘤坏死因子受体超家族成员5(TNFRSF5)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5)

AB504Hu21 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

AB504Ra21 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

AB499Hu21 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)

AA813Hu21 肿瘤坏死因子受体超家族成员12A(TNFRSF12A)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)

AA818Ra21 肿瘤坏死因子受体超家族成员10B(TNFRSF10B)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B)

AD934Hu21 肿瘤坏死因子配体超家族成员9(TNFSF9)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9)

AB251Hu22 肿瘤坏死因子配体超家族成员7(TNFSF7)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7)

AA850Hu22 肿瘤坏死因子配体超家族成员4(TNFSF4)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4)

AA827Hu21 肿瘤坏死因子配体超家族成员14(TNFSF14)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14)

AB750Hu22 肿瘤坏死因子配体超家族成员13(TNFSF13)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)

AB750Ca21 肿瘤坏死因子配体超家族成员13(TNFSF13)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)

AB750Ra21 肿瘤坏死因子配体超家族成员13(TNFSF13)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)

AD933Ra21 肿瘤坏死因子配体超家族成员12(TNFSF12)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12)

AA134Hu22 肿瘤坏死因子β(TNFβ)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Beta (TNFb)

AA134Bo21 肿瘤坏死因子β(TNFβ)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Beta (TNFb)

AA134Po21 肿瘤坏死因子β(TNFβ)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Beta (TNFb)

AA134Ra21 肿瘤坏死因子β(TNFβ)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Beta (TNFb)

AA134Rb21 肿瘤坏死因子β(TNFβ)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Beta (TNFb)

AC796Hu21 肿瘤坏死因子α诱导蛋白3(TNFαIP3)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3)

AF802Hu21 肿瘤坏死因子α诱导蛋白2(TNFαIP2)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 2 (TNFaIP2)

AA133Hu22 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Si21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Ca21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Po21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Rb21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Bo21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Cp21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Eq21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Gu21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Ov21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Ra21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Mu21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA993Hu21 促微管蛋白聚合蛋白(TPPP)单克隆抗体 Monoclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP)

AE711Hu21 微管蛋白β3(TUBβ3)单克隆抗体 Monoclonal Antibody to Tubulin Beta 3 (TUBb3)

AE260Hu21 色氨酸-2,3-双加氧酶(TDO)单克隆抗体 Monoclonal Antibody to Tryptophan-2,3-dioxygenase (TDO)

AG343Hu21 色氨酸羟化酶2(TPH2)单克隆抗体 Monoclonal Antibody to Tryptophan Hydroxylase 2 (TPH2)

AG343Ra21 色氨酸羟化酶2(TPH2)单克隆抗体 Monoclonal Antibody to Tryptophan Hydroxylase 2 (TPH2)

AG342Hu21 色氨酸羟化酶1(TPH1)单克隆抗体 Monoclonal Antibody to Tryptophan Hydroxylase 1 (TPH1)

AG342Ra21 色氨酸羟化酶1蛋白激酶MST抗体(TPH1)单克隆抗体 Monoclonal Antibody to Tryptophan Hydroxylase 1 (TPH1)

AD781Hu21 类胰蛋白酶β2(TPSβ2)单克隆抗体 Monoclonal Antibody to Tryptase Beta 2 (TPSb2)

AB070Hu22 类胰蛋白酶(TPS)单克隆抗体 Monoclonal Antibody to Tryptase (TPS)

AB070Ca21 类胰蛋白酶(TPS)单克隆抗体 Monoclonal Antibody to Tryptase (TPS)

AB070Eq21 类胰蛋白酶(TPS)单克隆抗体 Monoclonal Antibody to Tryptase (TPS)

AB070Po21 类胰蛋白酶(TPS)单克隆抗体 Monoclonal Antibody to Tryptase (TPS)