丙酮酸激酶-M2

丙酮酸激酶-M2 

『别名』CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; OPA interacting protein 3; Opa interactivity protein; PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PKM2; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozyme R/L; Pyruvate kinase isozymes M1/M2; Pyruvate kinase liver and blood cell; Pyruvate kinase liver and RBC; Pyruvate kinase liver and RBC type; Pyruvate kinase M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase type L; R type/L type pyruvate kinase; Red cell/liver pyruvate kinase; RPK; TCB; THBP 1; THBP1; Thyroid hormone binding protein cytosolic; KPYM_HUMAN. 

『浓度』丙酮酸激酶-M21mg/1ml 

『规格』0.1ml/100μg  0.2ml/200μg     

『抗体来源』Mouse  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

丙酮酸激酶-M2洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

丙酮酸激酶-M2其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAK204Rb52 血小板反应蛋白解整合素金属肽酶4(ADAMTS4)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

PAK207Bo01 血小板反应蛋白解整合素金属肽酶2(ADAMTS2)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 2 (ADAMTS2)

PAK207Mu01 血小板反应蛋白解整合素金属肽酶2(ADAMTS2)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 2 (ADAMTS2)

PAN899Hu01 血小板反应蛋白解整合素金属肽酶19(ADAMTS19)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 19 (ADAMTS19)

PAP002Hu01 血小板反应蛋白解整合素金属肽酶16(ADAMTS16)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 16 (ADAMTS16)

PAP226Mu01 血小板反应蛋白解整合素金属肽酶12(ADAMTS12)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

PAP226Mu02 血小板反应蛋白解整合素金属肽酶12(ADAMTS12)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

PAP226Hu01 血小板反应蛋白解整合素金属肽酶12(ADAMTS12)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

PAK210Ra01 血小板反应蛋白解整合素金属肽酶10(ADAMTS10)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 10 (ADAMTS10)

PAK210Hu01 血小板反应蛋白解整合素金属肽酶10(ADAMTS10)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 10 (ADAMTS10)

PAB973Hu01 血小板反应蛋白解整合素金属肽酶1(ADAMTS1)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

PAB973Ra01 血小板反应蛋白解整合素金属肽酶1(ADAMTS1)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

PAB973Mu01 血小板反应蛋白解整合素金属肽酶1(ADAMTS1)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

PAB973Hu02 血小板反应蛋白解整合素金属肽酶1(ADAMTS1)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

PAB973Mu02 血小板反应蛋白解整合素金属肽酶1(ADAMTS1)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

PAB973Hu03 血小板反应蛋白解整合素金属肽酶1(ADAMTS1)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

PAB973Ra02 血小板反应蛋白解整合素金属肽酶1(ADAMTS1)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

PAB829Hu01 解整合素金属蛋白酶9(ADAM9)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 9 (ADAM9)

PAB829Ra01 解整合素金属蛋白酶9(ADAM9)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 9 (ADAM9)

PAA620Hu01 解整合素金属蛋白酶8(ADAM8)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 8 (ADAM8)

PAA620Mu01 解整合素金属蛋白酶8(ADAM8)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 8 (ADAM8)

PAA620Ra01 解整合素金属蛋白酶8(ADAM8)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 8 (ADAM8)

PAA620Mu02 解整合素金属蛋白酶8(ADAM8)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 8 (ADAM8)

PAS445Ra01 解整合素金属蛋白酶6(ADAM6)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 6 (ADAM6)

PAS446Ra01 解整合素金属蛋白酶5(ADAM5)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 5 (ADAM5)

PAP196Hu01 解整合素金属蛋白酶33(ADAM33)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 33 (ADAM33)

PAK202Hu01 解整合素金属蛋白酶28(ADAM28)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 28 (ADAM28)

PAK165Hu01 解整合素金属蛋白酶22(ADAM22)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 22 (ADAM22)

PAQ207Hu01 解整合素金属蛋白酶20(ADAM20)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 20 (ADAM20)

PAK240Hu01 解整合素金属蛋白酶19(ADAM19)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 19 (ADAM19)

PAB555Hu01 解整合素金属蛋白酶17(ADAM17)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)

PAB555Ra01 解整合素金属蛋白酶17(ADAM17)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)

PAJ800Hu01 解整合素金属蛋白酶15(ADAM15)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 15 (ADAM15)

PAK242Hu01 解整合素金属蛋白酶12(ADAM12)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 12 (ADAM12)

PAA766Hu01 解整合素金属蛋白酶10(ADAM10)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

PAA766Ra01 解整合素金属蛋白酶10(ADAM10)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

PAA766Mu01 解整合素金属蛋白酶10(ADAM10)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

PAA766Mu02 解整合素金属蛋白酶10(ADAM10)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

PAS444Ra01 解整合素金属蛋白酶1(ADAM1)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloprotease 1 (ADAM1)

PAC270Hu01 7-脱氢胆固醇还原酶(DHCR7)多克隆抗体 Polyclonal Antibody to 7-Dehydrocholesterol Reductase (DHCR7)

PAB250Hu01 胞外5'-核苷酸酶(NT5E)多克隆抗体 Polyclonal Antibody to 5'-Nucleotidase, Ecto (NT5E)

PAB250Mu01 胞外5'-核苷酸酶(NT5E)多克隆抗体 Polyclonal Antibody to 5'-Nucleotidase, Ecto (NT5E)

PAB250Ra01 胞外5'-核苷酸酶(NT5E)多克隆抗体 Polyclonal Antibody to 5'-Nucleotidase, Ecto (NT5E)

PAB250Bo01 胞外5'-核苷酸酶(NT5E)多克隆抗体 Polyclonal Antibody to 5'-Nucleotidase, Ecto (NT5E)

PAD942Mu01 5'-核苷酸酶Ⅲ(NT5C3)多克隆抗体 Polyclonal Antibody to 5'-Nucleotidase, Cytosolic III (NT5C3)

PAD942Ra01 5'-核苷酸酶Ⅲ(NT5C3)多克隆抗体 Polyclonal Antibody to 5'-Nucleotidase, Cytosolic III (NT5C3)

PAD942Hu01 5'-核苷酸酶Ⅲ(NT5C3)多克隆抗体 Polyclonal Antibody to 5'-Nucleotidase, Cytosolic III (NT5C3)

PAG462Hu01 5-甲基四氢叶酸高半胱氨酸甲基转移酶(MTR)多克隆抗体 Polyclonal Antibody to 5-Methyltetrahydrofolate Homocysteine Methyltransferase (MTR)

PAD493Hu01 5-羟色胺受体4(HTR4)多克隆抗体 Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 (HTR4)

PAD488Ra01 5-羟色胺受体2C(HTR2C)多克隆抗体 Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2C (HTR2C)

PAD486Hu01 5-羟色胺受体2A(HTR2A)多克隆抗体 Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2A (HTR2A)

PAC271Hu01 5-羟色胺受体1A丙酮酸激酶-M2(HTR1A)多克隆抗体 Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1A (HTR1A)

PAA808Ge01 5-羟色胺(5-HT)多克隆抗体 Polyclonal Antibody to 5-Hydroxytryptamine (5-HT)

PAB005Ge01 5-羟基吲哚乙酸(5-HIAA)多克隆抗体 Polyclonal Antibody to 5-Hydroxyindoleacetic Acid (5-HIAA)

PAE251Hu01 4-羟基苯丙酮酸双加氧酶(HPD)多克隆抗体 Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)