神经上皮酪氨酸激酶/乳腺癌激酶10抗体

神经上皮酪氨酸激酶/乳腺癌激酶10抗体 

『别名』MCK10; CAK; CD 167; CD167; Cell adhesion kinase; DDR 1; DDR; DDR1; Discoidin domain receptor; Discoidin receptor tyrosine kinase isoform a; EDDR 1; Epithelial discoidin domain receptor 1; Epithelial specific receptor kinase; Mammarian carcinoma kinase 10; Neuroepithelial tyrosine kinase; Neurotrophic tyrosine kinase receptor type 4; NTRK 4; PTK 3; PTK 3A protein tyrosine kinase 3A; Receptor tyrosine kinase NEP; RTK 6; TRK E; Tyrosine kinase receptor E 

『浓度』神经上皮酪氨酸激酶/乳腺癌激酶10抗体1mg/1ml 

『规格』0.1ml/100μg  0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

神经上皮酪氨酸激酶/乳腺癌激酶10抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

神经上皮酪氨酸激酶/乳腺癌激酶10抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAB963Ge01 别孕烯醇酮(AP)多克隆抗体 Polyclonal Antibody to Allopregnanolone (AP)

PAC288Hu01 同种异体移植炎症因子1(AIF1)多克隆抗体 Polyclonal Antibody to Allograft Inflammatory Factor 1 (AIF1)

PAC288Mu01 同种异体移植炎症因子1(AIF1)多克隆抗体 Polyclonal Antibody to Allograft Inflammatory Factor 1 (AIF1)

PAC288Ra01 同种异体移植炎症因子1(AIF1)多克隆抗体 Polyclonal Antibody to Allograft Inflammatory Factor 1 (AIF1)

PAC288Mu02 同种异体移植炎症因子1(AIF1)多克隆抗体 Polyclonal Antibody to Allograft Inflammatory Factor 1 (AIF1)

PAC288Hu02 同种异体移植炎症因子1(AIF1)多克隆抗体 Polyclonal Antibody to Allograft Inflammatory Factor 1 (AIF1)

PAC288Gu01 同种异体移植炎症因子1(AIF1)多克隆抗体 Polyclonal Antibody to Allograft Inflammatory Factor 1 (AIF1)

PAA801Hu01 碱性鞘磷脂磷酸二酯酶(Alk-Smase)多克隆抗体 Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase)

PAA801Mu01 碱性鞘磷脂磷酸二酯酶(Alk-Smase)多克隆抗体 Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase)

PAA801Ra01 碱性鞘磷脂磷酸二酯酶(Alk-Smase)多克隆抗体 Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase)

PAA801Mu02 碱性鞘磷脂磷酸二酯酶(Alk-Smase)多克隆抗体 Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase)

PAJ649Hu01 胎盘碱性磷酸酶样蛋白2(ALPPL2)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2)

PAB219Hu01 胎盘碱性磷酸酶(ALPP)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase, Placental (ALPP)

PAB219Ra01 胎盘碱性磷酸酶(ALPP)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase, Placental (ALPP)

PAB091Hu01 肝肾骨碱性磷酸酶(ALPL)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)

PAB091Mu01 肝肾骨碱性磷酸酶(ALPL)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)

PAB091Ra01 肝肾骨碱性磷酸酶(ALPL)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)

PAB091Mu02 肝肾骨碱性磷酸酶(ALPL)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)

PAB085Hu01 肠碱性磷酸酶(ALPI)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase, Intestinal (ALPI)

PAB085Ra01 肠碱性磷酸酶(ALPI)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase, Intestinal (ALPI)

PAB472Hu01 碱性磷酸酶(ALP)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase (ALP)

PAB472Mu01 碱性磷酸酶(ALP)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase (ALP)

PAB472Ra01 碱性磷酸酶(ALP)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase (ALP)

PAB472Bo01 碱性磷酸酶(ALP)多克隆抗体 Polyclonal Antibody to Alkaline Phosphatase (ALP)

PAA987Ra01 醛固酮合酶(ALDOS)多克隆抗体 Polyclonal Antibody to Aldosterone Synthase (ALDOS)

PAQ201Hu01 醛糖还原酶相似蛋白1(ARL1)多克隆抗体 Polyclonal Antibody to Aldose Reductase Like Protein 1 (ARL1)

PAD320Hu01 果糖二磷酸醛缩酶C(ALDOC)多克隆抗体 Polyclonal Antibody to Aldolase C, Fructose Bisphosphate (ALDOC)

PAD320Ra01 果糖二磷酸醛缩酶C(ALDOC)多克隆抗体 Polyclonal Antibody to Aldolase C, Fructose Bisphosphate (ALDOC)

PAD319Ra01 果糖二磷酸醛缩酶B(ALDOB)多克隆抗体 Polyclonal Antibody to Aldolase B, Fructose Bisphosphate (ALDOB)

PAD319Hu01 果糖二磷酸醛缩酶B(ALDOB)多克隆抗体 Polyclonal Antibody to Aldolase B, Fructose Bisphosphate (ALDOB)

PAA642Ra01 果糖二磷酸醛缩酶A(ALDOA)多克隆抗体 Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA)

PAA642Hu01 果糖二磷酸醛缩酶A(ALDOA)多克隆抗体 Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA)

PAQ195Bo01 乙醛氧化酶1(AOX1)多克隆抗体 Polyclonal Antibody to Aldehyde Oxidase 1 (AOX1)

PAC024Hu01 线粒体乙醛脱氢酶(ALDM)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase, Mitochondrial (ALDM)

PAC024Mu01 线粒体乙醛脱氢酶(ALDM)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase, Mitochondrial (ALDM)

PAC024Ra01 线粒体乙醛脱氢酶(ALDM)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase, Mitochondrial (ALDM)

PAE839Mu01 醛脱氢酶9家族成员A1(ALDH9A1)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1)

PAE839Mu02 醛脱氢酶9家族成员A1(ALDH9A1)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1)

PAE837Hu01 醛脱氢酶7家族成员A1(ALDH7A1)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)

PAE837Mu01 醛脱氢酶7家族成员A1(ALDH7A1)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)

PAE837Ra01 醛脱氢酶7家族成员A1(ALDH7A1)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)

PAE830Hu01 醛脱氢酶3家族成员A1(ALDH3A1)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1)

PAE826Hu01 醛脱氢酶1家族成员A3(ALDH1A3)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3)

PAE825Hu01 醛脱氢酶1家族成员A2(ALDH1A2)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2)

PAE824Hu01 醛脱氢酶1家族成员A1(ALDH1A1)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)

PAE824Mu01 醛脱氢酶1家族成员A1(ALDH1A1)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)

PAE824Mu02 醛脱氢酶1家族成员A1(ALDH1A1)多克隆抗体 Polyclonal Antibody to Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)

PAE866Mu01 乙醇脱氢酶7(ADH7)多克隆抗体 Polyclonal Antibody to Alcohol Dehydrogenase 7 (ADH7)

PAE861Hu01 乙醇脱氢酶2神经上皮酪氨酸激酶/乳腺癌激酶10抗体(ADH2)多克隆抗体 Polyclonal Antibody to Alcohol Dehydrogenase 2 (ADH2)

PAA681Hu01 乙醇脱氢酶1(ADH1)多克隆抗体 Polyclonal Antibody to Alcohol Dehydrogenase 1 (ADH1)

PAA681Mu01 乙醇脱氢酶1(ADH1)多克隆抗体 Polyclonal Antibody to Alcohol Dehydrogenase 1 (ADH1)

PAA681Ra01 乙醇脱氢酶1(ADH1)多克隆抗体 Polyclonal Antibody to Alcohol Dehydrogenase 1 (ADH1)