高度保守基因相关蛋白Membralin抗体

?高度保守基因相关蛋白Membralin抗体 

『别名』ASBABP1; C19orf6; MGC4022; R32184_3; aspecific BCL2 ARE-binding protein 1; C19orf6; chromosome 19 open reading frame 6; MBRL; MBRL_HUMAN. 

『浓度』高度保守基因相关蛋白Membralin抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

高度保守基因相关蛋白Membralin抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

高度保守基因相关蛋白Membralin抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAA821Hu01 C反应蛋白(CRP)多克隆抗体 Polyclonal Antibody to C Reactive Protein (CRP)

PAA821Hu02 C反应蛋白(CRP)多克隆抗体 Polyclonal Antibody to C Reactive Protein (CRP)

PAC348Hu01 丁酰胆碱酯酶(BCHE)多克隆抗体 Polyclonal Antibody to Butyrylcholinesterase (BCHE)

PAC348Hu02 丁酰胆碱酯酶(BCHE)多克隆抗体 Polyclonal Antibody to Butyrylcholinesterase (BCHE)

PAC348Ra01 丁酰胆碱酯酶(BCHE)多克隆抗体 Polyclonal Antibody to Butyrylcholinesterase (BCHE)

PAC347Hu01 嗜乳脂蛋白亚家族1成员A1(BTN1A1)多克隆抗体 Polyclonal Antibody to Butyrophilin Subfamily 1, Member A1 (BTN1A1)

PAJ564Hu01 BTG3关联核蛋白(BANP)多克隆抗体 Polyclonal Antibody to BTG3 Associated Nuclear Protein (BANP)

PAB915Hu01 布鲁东氏酪氨酸激酶(Btk)多克隆抗体 Polyclonal Antibody to Bruton'S Tyrosine Kinase (Btk)

PAB915Ra01 布鲁东氏酪氨酸激酶(Btk)多克隆抗体 Polyclonal Antibody to Bruton'S Tyrosine Kinase (Btk)

PAB915Mu01 布鲁东氏酪氨酸激酶(Btk)多克隆抗体 Polyclonal Antibody to Bruton'S Tyrosine Kinase (Btk)

PAL260Mu01 CDO兄弟同源物(BOC)多克隆抗体 Polyclonal Antibody to Brother Of CDO Homolog (BOC)

PAC253Mu01 含溴区蛋白8(BRD8)多克隆抗体 Polyclonal Antibody to Bromodomain Containing Protein 8 (BRD8)

PAJ554Hu01 桥连整合因子2(BIN2)多克隆抗体 Polyclonal Antibody to Bridging Integrator 2 (BIN2)

PAJ555Hu01 桥连整合因子1(BIN1)多克隆抗体 Polyclonal Antibody to Bridging Integrator 1 (BIN1)

PAC177Hu01 短蛋白聚糖(BCAN)多克隆抗体 Polyclonal Antibody to Brevican (BCAN)

PAC177Mu01 短蛋白聚糖(BCAN)多克隆抗体 Polyclonal Antibody to Brevican (BCAN)

PAF221Hu01 乳腺癌扩增序列3(BCAS3)多克隆抗体 Polyclonal Antibody to Breast Carcinoma Amplified Sequence 3 (BCAS3)

PAB364Hu01 乳腺癌易感蛋白2(BRCA2)多克隆抗体 Polyclonal Antibody to Breast Cancer Susceptibility Protein 2 (BRCA2)

PAB364Mu01 乳腺癌易感蛋白2(BRCA2)多克隆抗体 Polyclonal Antibody to Breast Cancer Susceptibility Protein 2 (BRCA2)

PAB364Mu02 乳腺癌易感蛋白2(BRCA2)多克隆抗体 Polyclonal Antibody to Breast Cancer Susceptibility Protein 2 (BRCA2)

PAB364Ra01 乳腺癌易感蛋白2(BRCA2)多克隆抗体 Polyclonal Antibody to Breast Cancer Susceptibility Protein 2 (BRCA2)

PAB376Hu01 乳腺癌易感蛋白1(BRCA1)多克隆抗体 Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1)

PAB376Ra01 乳腺癌易感蛋白1(BRCA1)多克隆抗体 Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1)

PAB376Mu01 乳腺癌易感蛋白1(BRCA1)多克隆抗体 Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1)

PAG768Hu01 乳腺癌抗雌激素药物耐药性基因3(BCAR3)多克隆抗体 Polyclonal Antibody to Breast Cancer Anti-Estrogen Resistance 3 (BCAR3)

PAG769Hu01 乳腺癌抗雌激素药物耐药性基因1(BCAR1)多克隆抗体 Polyclonal Antibody to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1)

PAB607Hu01 胸肾表达趋化因子(BRAK)多克隆抗体 Polyclonal Antibody to Breast And Kidney Expressed Chemokine (BRAK)

PAB607Mu01 胸肾表达趋化因子(BRAK)多克隆抗体 Polyclonal Antibody to Breast And Kidney Expressed Chemokine (BRAK)

PAB607Mu02 胸肾表达趋化因子(BRAK)多克隆抗体 Polyclonal Antibody to Breast And Kidney Expressed Chemokine (BRAK)

PAB607Ra01 胸肾表达趋化因子(BRAK)多克隆抗体 Polyclonal Antibody to Breast And Kidney Expressed Chemokine (BRAK)

PAN348Hu01 含BRCA1/BRCA2复合体3亚基(BRCC3)多克隆抗体 Polyclonal Antibody to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)

PAJ560Ra01 支链α-酮酸脱氢酶(BCKDK)多克隆抗体 Polyclonal Antibody to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK)

PAF233Hu01 脑特异性血管生长抑制因子3(BAI3)多克隆抗体 Polyclonal Antibody to Brain Specific Angiogenesis Inhibitor 3 (BAI3)

PAC346Hu01 脑特异性血管生长抑制因子1(BAI1)多克隆抗体 Polyclonal Antibody to Brain Specific Angiogenesis Inhibitor 1 (BAI1)

PAQ978Hu01 脑蛋白44样蛋白(BRP44L)多克隆抗体 Polyclonal Antibody to Brain Protein 44 Like Protein(BRP44L)

PAA541Hu01 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

PAA541Mu01 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

PAA541Ra01 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

PAA541Bo01 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

PAA541Po01 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

PAA541Ca01 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

PAA541Mu02 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

PAA541Hu02 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

PAA541Mu03 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

PAB093Mu01 脑指蛋白(BFP)多克隆抗体 Polyclonal Antibody to Brain Finger Protein (BFP)

PAB093Mu02 脑指蛋白(BFP)多克隆抗体 Polyclonal Antibody to Brain Finger Protein (BFP)

PAB093Ra01 脑指蛋白(BFP)多克隆抗体 Polyclonal Antibody to Brain Finger Protein (BFP)

PAB093Hu01 脑指蛋白(BFP)多克隆抗体 Polyclonal Antibody to Brain Finger Protein (BFP)

PAA011Bo01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

PAA011Ca01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

PAA011Eq01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

PAA011Ga01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

PAA011Gu01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

PAA011Hu01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

PAA011Mu01 脑源性神经营养因子高度保守基因相关蛋白Membralin抗体(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

PAA011Po01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

PAA011Ra01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

PAA011Rb52 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)