神经元膜糖蛋白锚定蛋白2抗体

?神经元膜糖蛋白锚定蛋白2抗体 

『别名』MAM domain-containing glycosylphosphatidylinositol anchor protein 2; MAM domain-containing protein 1; MAMDC1; Mdga2; MDGA2_HUMAN; UNQ8188/PRO23197; c14_5286. 

『浓度』神经元膜糖蛋白锚定蛋白2抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

神经元膜糖蛋白锚定蛋白2抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

神经元膜糖蛋白锚定蛋白2抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAB261Mu01 CD72分子(CD72)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 72 (CD72)

PAA952Hu01 CD6分子(CD6)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 6 (CD6)

PAA952Mu01 CD6分子(CD6)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 6 (CD6)

PAA952Ra01 CD6分子(CD6)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 6 (CD6)

PAB279Hu01 CD52分子(CD52)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 52 (CD52)

PAA119Bo01 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Ca01 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Fi01 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Ga01 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Hu01 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Mu01 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Po01 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Ra01 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Si01 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Hu02 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Po02 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Po03 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Mu02 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAA119Hu03 CD40配体(CD40L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

PAB167Hu01 CD4分子(CD4)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 4 (CD4)

PAB167Mu01 CD4分子(CD4)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 4 (CD4)

PAB167Si01 CD4分子(CD4)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 4 (CD4)

PAB167Mu02 CD4分子(CD4)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 4 (CD4)

PAB872Hu01 CD3d分子(CD3d)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 3d (CD3d)

PAB872Ov01 CD3d分子(CD3d)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 3d (CD3d)

PAB872Po01 CD3d分子(CD3d)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 3d (CD3d)

PAB872Ra01 CD3d分子(CD3d)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 3d (CD3d)

PAB872Mu01 CD3d分子(CD3d)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 3d (CD3d)

PAB872Bo01 CD3d分子(CD3d)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 3d (CD3d)

PAB959Hu01 CD34分子(CD34)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 34 (CD34)

PAB959Mu01 CD34分子(CD34)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 34 (CD34)

PAB959Mu02 CD34分子(CD34)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 34 (CD34)

PAB007Ra01 CD320分子(CD320)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 320 (CD320)

PAA118Ga01 CD30配体(CD30L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 30 Ligand (CD30L)

PAA118Hu01 CD30配体(CD30L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 30 Ligand (CD30L)

PAA118Mu01 CD30配体(CD30L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 30 Ligand (CD30L)

PAA118Ra01 CD30配体(CD30L)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 30 Ligand (CD30L)

PAA652Mu01 CD28分子(CD28)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 28 (CD28)

PAA781Hu01 CD276分子(CD276)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)

PAA781Mu01 CD276分子(CD276)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)

PAB143Hu01 CD24分子(CD24)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 24 (CD24)

PAB669Hu01 CD226分子(CD226)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 226 (CD226)

PAA880Hu01 CD200分子(CD200)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 200 (CD200)

PAB170Hu01 CD1e分子(CD1e)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 1e (CD1e)

PAB172Hu01 CD1d分子(CD1d)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 1d (CD1d)

PAB172Mu01 CD1d分子(CD1d)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 1d (CD1d)

PAB172Ra01 CD1d分子(CD1d)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 1d (CD1d)

PAB873Hu01 CD19分子(CD19)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 19 (CD19)

PAB873Mu01 CD19分子(CD19)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 19 (CD19)

PAB873Ra01 CD19分子(CD19)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 19 (CD19)

PAB873Mu02 CD19分子(CD19)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 19 (CD19)

PAA989Hu01 CD160分子(CD160)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 160 (CD160)

PAA685Hu01 CD14分子(CD14)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 14 (CD14)

PAA685Ra01 CD14分子神经元膜糖蛋白锚定蛋白2抗体(CD14)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 14 (CD14)

PAA685Mu01 CD14分子(CD14)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 14 (CD14)

PAA685Mu02 CD14分子(CD14)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 14 (CD14)

PAA685Po01 CD14分子(CD14)多克隆抗体 Polyclonal Antibody to Cluster Of Differentiation 14 (CD14)