环指蛋白60抗体

环指蛋白60抗体 

『别名』FXY2; MID2; Midin-2; Midline 2; Midline defect 2; Midline-2; Probable E3 ubiquitin-protein ligase MID2; RING finger protein 60; Ring finger protein protein 60; RNF60; TRIM1; TRIM1_HUMAN; Tripartite motif protein 1; Tripartite motif-containing protein 1; Tripartite motif-containing protein 1; Midline defect 2; FLJ37715; FLJ41813. 

『浓度』环指蛋白60抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

环指蛋白60抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

环指蛋白60抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAA389Ra01 补体成分4a(C4a)多克隆抗体 Polyclonal Antibody to Complement Component 4a (C4a)

PAA389Hu01 补体成分4a(C4a)多克隆抗体 Polyclonal Antibody to Complement Component 4a (C4a)

PAA389Mu02 补体成分4a(C4a)多克隆抗体 Polyclonal Antibody to Complement Component 4a (C4a)

PAA888Hu01 补体成分4(C4)多克隆抗体 Polyclonal Antibody to Complement Component 4 (C4)

PAA888Mu01 补体成分4(C4)多克隆抗体 Polyclonal Antibody to Complement Component 4 (C4)

PAA888Ra01 补体成分4(C4)多克隆抗体 Polyclonal Antibody to Complement Component 4 (C4)

PAA888Ga01 补体成分4(C4)多克隆抗体 Polyclonal Antibody to Complement Component 4 (C4)

PAA387Mu01 补体成分3a(C3a)多克隆抗体 Polyclonal Antibody to Complement Component 3a (C3a)

PAA387Ra01 补体成分3a(C3a)多克隆抗体 Polyclonal Antibody to Complement Component 3a (C3a)

PAA387Hu01 补体成分3a(C3a)多克隆抗体 Polyclonal Antibody to Complement Component 3a (C3a)

PAA387Po01 补体成分3a(C3a)多克隆抗体 Polyclonal Antibody to Complement Component 3a (C3a)

PAA861Hu01 补体成分3(C3)多克隆抗体 Polyclonal Antibody to Complement Component 3 (C3)

PAA861Mu01 补体成分3(C3)多克隆抗体 Polyclonal Antibody to Complement Component 3 (C3)

PAA861Po01 补体成分3(C3)多克隆抗体 Polyclonal Antibody to Complement Component 3 (C3)

PAA861Ra01 补体成分3(C3)多克隆抗体 Polyclonal Antibody to Complement Component 3 (C3)

PAA861Ga01 补体成分3(C3)多克隆抗体 Polyclonal Antibody to Complement Component 3 (C3)

PAA861Mu02 补体成分3(C3)多克隆抗体 Polyclonal Antibody to Complement Component 3 (C3)

PAA862Hu01 补体成分2(C2)多克隆抗体 Polyclonal Antibody to Complement Component 2 (C2)

PAA862Ra01 补体成分2(C2)多克隆抗体 Polyclonal Antibody to Complement Component 2 (C2)

PAB674Hu01 补体成分1s(C1s)多克隆抗体 Polyclonal Antibody to Complement Component 1, S Subcomponent (C1s)

PAB112Hu01 补体成分1r(C1r)多克隆抗体 Polyclonal Antibody to Complement Component 1, R Subcomponent (C1r)

PAE867Hu01 补体成分1q子成分样蛋白1(C1qL1)多克隆抗体 Polyclonal Antibody to Complement Component 1, Q Subcomponent Like Protein 1 (C1qL1)

PAE869Hu01 补体成分1q子成分C(C1qC)多克隆抗体 Polyclonal Antibody to Complement Component 1, Q Subcomponent C (C1qC)

PAD207Hu01 补体成分1q子成分A(C1qA)多克隆抗体 Polyclonal Antibody to Complement Component 1, Q Subcomponent A (C1qA)

PAD207Ra01 补体成分1q子成分A(C1qA)多克隆抗体 Polyclonal Antibody to Complement Component 1, Q Subcomponent A (C1qA)

PAD207Mu01 补体成分1q子成分A(C1qA)多克隆抗体 Polyclonal Antibody to Complement Component 1, Q Subcomponent A (C1qA)

PAB405Hu01 补体成分1q受体(C1qR1)多克隆抗体 Polyclonal Antibody to Complement Component 1, Q Receptor (C1qR1)

PAB405Ra01 补体成分1q受体(C1qR1)多克隆抗体 Polyclonal Antibody to Complement Component 1, Q Receptor (C1qR1)

PAB405Mu01 补体成分1q受体(C1qR1)多克隆抗体 Polyclonal Antibody to Complement Component 1, Q Receptor (C1qR1)

PAA758Hu01 补体C3转化酶(C3c)多克隆抗体 Polyclonal Antibody to Complement C3 Convertase (C3c)

PAA758Mu01 补体C3转化酶(C3c)多克隆抗体 Polyclonal Antibody to Complement C3 Convertase (C3c)

PAA747Hu01 补体1q(C1q)多克隆抗体 Polyclonal Antibody to Complement 1q (C1q)

PAA235Hu01 补体1抑制因子(C1INH)多克隆抗体 Polyclonal Antibody to Complement 1 Inhibitor (C1INH)

PAA235Mu01 补体1抑制因子(C1INH)多克隆抗体 Polyclonal Antibody to Complement 1 Inhibitor (C1INH)

PAA235Ra01 补体1抑制因子(C1INH)多克隆抗体 Polyclonal Antibody to Complement 1 Inhibitor (C1INH)

PAA235Rb51 补体1抑制因子(C1INH)多克隆抗体 Polyclonal Antibody to Complement 1 Inhibitor (C1INH)

PAA235Ra02 补体1抑制因子(C1INH)多克隆抗体 Polyclonal Antibody to Complement 1 Inhibitor (C1INH)

PAA235Mu03 补体1抑制因子(C1INH)多克隆抗体 Polyclonal Antibody to Complement 1 Inhibitor (C1INH)

PAA235Mu02 补体1抑制因子(C1INH)多克隆抗体 Polyclonal Antibody to Complement 1 Inhibitor (C1INH)

PAB484Hu01 巨噬细胞集落刺激因子受体(MCSFR)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor Receptor, Macrophage (MCSFR)

PAB484Mu01 巨噬细胞集落刺激因子受体(MCSFR)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor Receptor, Macrophage (MCSFR)

PAB484Ra01 巨噬细胞集落刺激因子受体(MCSFR)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor Receptor, Macrophage (MCSFR)

PAB476Hu01 粒细胞集落刺激因子受体(GCSFR)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor Receptor, Granulocyte (GCSFR)

PAB476Hu02 粒细胞集落刺激因子受体(GCSFR)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor Receptor, Granulocyte (GCSFR)

PAB476Mu01 粒细胞集落刺激因子受体(GCSFR)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor Receptor, Granulocyte (GCSFR)

PAB476Ra01 粒细胞集落刺激因子受体(GCSFR)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor Receptor, Granulocyte (GCSFR)

PAA042Hu01 粒细胞集落刺激因子(GCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

PAA042Mu01 粒细胞集落刺激因子(GCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

PAA042Ra01 粒细胞集落刺激因子(GCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

PAA042Po01 粒细胞集落刺激因子(GCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

PAA042Mu02 粒细胞集落刺激因子(GCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

PAA042Bo01 粒细胞集落刺激因子(GCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

PAA045Ca01 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAA045Hu01 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAA045Mu01 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAA045Ra01 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAA045Mu02 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAB535Hu01 集落刺激因子2受体β(CSF2Rβ)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb)

PAB487Mu01 集落刺激因子2受体α(CSF2Rα)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra)

PAB487Hu01 集落刺激因子2受体α环指蛋白60抗体(CSF2Rα)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra)

PAB487Hu02 集落刺激因子2受体α(CSF2Rα)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra)

PAA090Hu01 巨噬细胞集落刺激因子(MCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 1, Macrophage (MCSF)

PAA090Mu01 巨噬细胞集落刺激因子(MCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 1, Macrophage (MCSF)