肌肉特定环指蛋白3抗体

?肌肉特定环指蛋白3抗体 

『别名』MURF 3; MURF; MURF-3; MuRF3; Muscle specific RING finger protein 3; Muscle specific RING finger protein; Muscle specific RING finger protein homolog; Muscle-specific RING finger protein 3; Muscle-specific RING finger protein; Ring finger protein 30; RNF30; TRI54_HUMAN; TRIM 54; TRIM54; RNF28; Tripartite motif containing 54; tibody Tripartite motif-containing protein 54. 

『浓度』肌肉特定环指蛋白3抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

肌肉特定环指蛋白3抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

肌肉特定环指蛋白3抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAA559Ra01 肠型脂肪酸结合蛋白(FABP2)多克隆抗体 Polyclonal Antibody to Fatty Acid Binding Protein 2, Intestinal (FABP2)

PAA559Mu02 肠型脂肪酸结合蛋白(FABP2)多克隆抗体 Polyclonal Antibody to Fatty Acid Binding Protein 2, Intestinal (FABP2)

PAB566Hu01 肝型脂肪酸结合蛋白(FABP1)多克隆抗体 Polyclonal Antibody to Fatty Acid Binding Protein 1, Liver (FABP1)

PAB566Po01 肝型脂肪酸结合蛋白(FABP1)多克隆抗体 Polyclonal Antibody to Fatty Acid Binding Protein 1, Liver (FABP1)

PAB566Ga01 肝型脂肪酸结合蛋白(FABP1)多克隆抗体 Polyclonal Antibody to Fatty Acid Binding Protein 1, Liver (FABP1)

PAB566Ra01 肝型脂肪酸结合蛋白(FABP1)多克隆抗体 Polyclonal Antibody to Fatty Acid Binding Protein 1, Liver (FABP1)

PAB566Mu01 肝型脂肪酸结合蛋白(FABP1)多克隆抗体 Polyclonal Antibody to Fatty Acid Binding Protein 1, Liver (FABP1)

PAB566Bo01 肝型脂肪酸结合蛋白(FABP1)多克隆抗体 Polyclonal Antibody to Fatty Acid Binding Protein 1, Liver (FABP1)

PAE672Mu01 肌动蛋白束蛋白2(FSCN2)多克隆抗体 Polyclonal Antibody to Fascin 2 (FSCN2)

PAE672Hu01 肌动蛋白束蛋白2(FSCN2)多克隆抗体 Polyclonal Antibody to Fascin 2 (FSCN2)

PAE672Mu02 肌动蛋白束蛋白2(FSCN2)多克隆抗体 Polyclonal Antibody to Fascin 2 (FSCN2)

PAB757Hu01 肌动蛋白束蛋白(FSCN)多克隆抗体 Polyclonal Antibody to Fascin (FSCN)

PAB757Mu01 肌动蛋白束蛋白(FSCN)多克隆抗体 Polyclonal Antibody to Fascin (FSCN)

PAB757Ra01 肌动蛋白束蛋白(FSCN)多克隆抗体 Polyclonal Antibody to Fascin (FSCN)

PAK078Mu01 含Fas关联死亡域蛋白(FADD)多克隆抗体 Polyclonal Antibody to Fas Associating Death Domain Containing Protein (FADD)

PAK078Hu01 含Fas关联死亡域蛋白(FADD)多克隆抗体 Polyclonal Antibody to Fas Associating Death Domain Containing Protein (FADD)

PAK403Hu01 Fas凋亡抑制分子3(FAIM3)多克隆抗体 Polyclonal Antibody to Fas Apoptotic Inhibitory Molecule 3 (FAIM3)

PAG487Hu01 法尼基转移酶α(FNTα)多克隆抗体 Polyclonal Antibody to Farnesyltransferase Alpha (FNTa)

PAE162Ra01 法尼基二磷酸合酶(FDPS)多克隆抗体 Polyclonal Antibody to Farnesyl Diphosphate Synthase (FDPS)

PAE162Hu01 法尼基二磷酸合酶(FDPS)多克隆抗体 Polyclonal Antibody to Farnesyl Diphosphate Synthase (FDPS)

PAB708Hu01 法尼基二磷酸法尼基转移酶1(FDFT1)多克隆抗体 Polyclonal Antibody to Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1)

PAB708Mu01 法尼基二磷酸法尼基转移酶1(FDFT1)多克隆抗体 Polyclonal Antibody to Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1)

PAB708Ra01 法尼基二磷酸法尼基转移酶1(FDFT1)多克隆抗体 Polyclonal Antibody to Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1)

PAC042Hu01 法尼酯X受体(FXR)多克隆抗体 Polyclonal Antibody to Farnesoid X Receptor (FXR)

PAC042Mu01 法尼酯X受体(FXR)多克隆抗体 Polyclonal Antibody to Farnesoid X Receptor (FXR)

PAC042Mu02 法尼酯X受体(FXR)多克隆抗体 Polyclonal Antibody to Farnesoid X Receptor (FXR)

PAC042Ra01 法尼酯X受体(FXR)多克隆抗体 Polyclonal Antibody to Farnesoid X Receptor (FXR)

PAR030Hu01 序列相似家族5成员C(FAM5C)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 5, Member C (FAM5C)

PAK368Hu01 序列相似家族3成员D(FAM3D)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member D (FAM3D)

PAL506Hu01 序列相似家族3成员C(FAM3C)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member C (FAM3C)

PAL507Hu01 序列相似家族3成员B(FAM3B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member B (FAM3B)

PAL507Mu01 序列相似家族3成员B(FAM3B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member B (FAM3B)

PAL507Mu02 序列相似家族3成员B(FAM3B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member B (FAM3B)

PAK371Hu01 序列相似家族20成员A(FAM20A)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 20, Member A (FAM20A)

PAQ976Hu01 序列相似家族19成员A4(FAM19A4)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 19, Member A4 (FAM19A4)

PAQ975Hu01 序列相似家族19成员A3(FAM19A3)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 19, Member A3 (FAM19A3)

PAQ975Mu01 序列相似家族19成员A3(FAM19A3)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 19, Member A3 (FAM19A3)

PAQ974Mu01 序列相似家族19成员A2(FAM19A2)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 19, Member A2 (FAM19A2)

PAR758Mu01 序列相似家族135成员B(FAM135B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 135, Member B (FAM135B)

PAR758Mu02 序列相似家族135成员B(FAM135B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 135, Member B (FAM135B)

PAV122Hu01 序列相似家族132成员A(FAM132A)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 132, Member A (FAM132A)

PAV122Hu02 序列相似家族132成员A(FAM132A)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 132, Member A (FAM132A)

PAA031Hu01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)

PAA031Mu01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)

PAA031Po01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)

PAA031Ra01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)

PAA031Si01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)

PAA031Hu02 凋亡相关因子配体肌肉特定环指蛋白3抗体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)

PAA031Mu02 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)

PAA030Bo01 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)

PAA030Hu01 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)

PAA030Mu01 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)