微原纤维胶原相关蛋白1抗体

微原纤维胶原相关蛋白1抗体 

『别名』MFAP1 is an extracellular protein associated with elastic fiber microfibrils. 

『浓度』微原纤维胶原相关蛋白1抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

微原纤维胶原相关蛋白1抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

微原纤维胶原相关蛋白1抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAA097Ra01 基质金属蛋白酶1(MMP1)多克隆抗体 Polyclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

PAA097Rb51 基质金属蛋白酶1(MMP1)多克隆抗体 Polyclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

PAA097Mu02 基质金属蛋白酶1(MMP1)多克隆抗体 Polyclonal Antibody to Matrix Metalloproteinase 1 (MMP1)

PAB477Hu01 基质Gla蛋白(MGP)多克隆抗体 Polyclonal Antibody to Matrix Gla Protein (MGP)

PAB477Ra01 基质Gla蛋白(MGP)多克隆抗体 Polyclonal Antibody to Matrix Gla Protein (MGP)

PAB477Mu01 基质Gla蛋白(MGP)多克隆抗体 Polyclonal Antibody to Matrix Gla Protein (MGP)

PAB477Ra02 基质Gla蛋白(MGP)多克隆抗体 Polyclonal Antibody to Matrix Gla Protein (MGP)

PAB477Mu02 基质Gla蛋白(MGP)多克隆抗体 Polyclonal Antibody to Matrix Gla Protein (MGP)

PAB477Rb51 基质Gla蛋白(MGP)多克隆抗体 Polyclonal Antibody to Matrix Gla Protein (MGP)

PAB477Bo01 基质Gla蛋白(MGP)多克隆抗体 Polyclonal Antibody to Matrix Gla Protein (MGP)

PAB477Po01 基质Gla蛋白(MGP)多克隆抗体 Polyclonal Antibody to Matrix Gla Protein (MGP)

PAB232Hu01 细胞外基质磷酸糖蛋白(MEPE)多克隆抗体 Polyclonal Antibody to Matrix Extracellular Phosphoglycoprotein (MEPE)

PAB232Mu01 细胞外基质磷酸糖蛋白(MEPE)多克隆抗体 Polyclonal Antibody to Matrix Extracellular Phosphoglycoprotein (MEPE)

PAB232Ra01 细胞外基质磷酸糖蛋白(MEPE)多克隆抗体 Polyclonal Antibody to Matrix Extracellular Phosphoglycoprotein (MEPE)

PAB232Mu02 细胞外基质磷酸糖蛋白(MEPE)多克隆抗体 Polyclonal Antibody to Matrix Extracellular Phosphoglycoprotein (MEPE)

PAH678Hu01 Matrin 3蛋白(MATR3)多克隆抗体 Polyclonal Antibody to Matrin 3 (MATR3)

PAE149Mu01 母系蛋白2(MATN2)多克隆抗体 Polyclonal Antibody to Matrilin 2 (MATN2)

PAE149Mu02 母系蛋白2(MATN2)多克隆抗体 Polyclonal Antibody to Matrilin 2 (MATN2)

PAH665Hu01 母系胚胎亮氨酸拉链蛋白激酶(MELK)多克隆抗体 Polyclonal Antibody to Maternal Embryonic Leucine Zipper Kinase (MELK)

PAH665Mu01 母系胚胎亮氨酸拉链蛋白激酶(MELK)多克隆抗体 Polyclonal Antibody to Maternal Embryonic Leucine Zipper Kinase (MELK)

PAH665Mu02 母系胚胎亮氨酸拉链蛋白激酶(MELK)多克隆抗体 Polyclonal Antibody to Maternal Embryonic Leucine Zipper Kinase (MELK)

PAH665Ra01 母系胚胎亮氨酸拉链蛋白激酶(MELK)多克隆抗体 Polyclonal Antibody to Maternal Embryonic Leucine Zipper Kinase (MELK)

PAL709Hu01 MARCKS相关蛋白(MARCKSL1)多克隆抗体 Polyclonal Antibody to MARCKS Related Protein (MARCKSL1)

PAS723Hu01 含MANSC域蛋白1(MANSC1)多克隆抗体 Polyclonal Antibody to MANSC Domain Containing Protein 1 (MANSC1)

PAH694Hu01 甘露糖苷酶α2B类成员1(MAN2B1)多克隆抗体 Polyclonal Antibody to Mannosidase Alpha Class 2B Member 1 (MAN2B1)

PAH689Hu01 甘露糖苷酶α1A类成员1(MAN1A1)多克隆抗体 Polyclonal Antibody to Mannosidase Alpha Class 1A Member 1 (MAN1A1)

PAH700Hu01 甘露糖-6-磷酸受体(M6PR)多克隆抗体 Polyclonal Antibody to Mannose-6-Phosphate Receptor (M6PR)

PAA733Hu01 甘露糖受体C2(MRC2)多克隆抗体 Polyclonal Antibody to Mannose Receptor C Type 2 (MRC2)

PAA733Mu01 甘露糖受体C2(MRC2)多克隆抗体 Polyclonal Antibody to Mannose Receptor C Type 2 (MRC2)

PAA733Mu02 甘露糖受体C2(MRC2)多克隆抗体 Polyclonal Antibody to Mannose Receptor C Type 2 (MRC2)

PAT618Hu01 甘露糖受体C1样蛋白1(MRC1)多克隆抗体 Polyclonal Antibody to Mannose Receptor C Type 1 Like Protein 1(MRC1)

PAB542Hu01 甘露糖受体C1(MRC1)多克隆抗体 Polyclonal Antibody to Mannose Receptor C Type 1 (MRC1)

PAB542Mu01 甘露糖受体C1(MRC1)多克隆抗体 Polyclonal Antibody to Mannose Receptor C Type 1 (MRC1)

PAB542Ra01 甘露糖受体C1(MRC1)多克隆抗体 Polyclonal Antibody to Mannose Receptor C Type 1 (MRC1)

PAB542Mu02 甘露糖受体C1(MRC1)多克隆抗体 Polyclonal Antibody to Mannose Receptor C Type 1 (MRC1)

PAH620Hu01 甘露糖磷酸异构酶(MPI)多克隆抗体 Polyclonal Antibody to Mannose Phosphate Isomerase (MPI)

PAB480Hu01 甘露糖结合蛋白(MBL)多克隆抗体 Polyclonal Antibody to Mannose Binding Lectin (MBL)

PAB480Mu01 甘露糖结合蛋白(MBL)多克隆抗体 Polyclonal Antibody to Mannose Binding Lectin (MBL)

PAB480Hu02 甘露糖结合蛋白(MBL)多克隆抗体 Polyclonal Antibody to Mannose Binding Lectin (MBL)

PAB480Ra01 甘露糖结合蛋白(MBL)多克隆抗体 Polyclonal Antibody to Mannose Binding Lectin (MBL)

PAB480Ra02 甘露糖结合蛋白(MBL)多克隆抗体 Polyclonal Antibody to Mannose Binding Lectin (MBL)

PAB480Ga01 甘露糖结合蛋白(MBL)多克隆抗体 Polyclonal Antibody to Mannose Binding Lectin (MBL)

PAB480Mu02 甘露糖结合蛋白(MBL)多克隆抗体 Polyclonal Antibody to Mannose Binding Lectin (MBL)

PAB480Hu03 甘露糖结合蛋白(MBL)多克隆抗体 Polyclonal Antibody to Mannose Binding Lectin (MBL)

PAA859Hu01 甘露聚糖结合凝集素关联丝氨酸蛋白酶2(MASP2)多克隆抗体 Polyclonal Antibody to Mannose Associated Serine Protease 2 (MASP2)

PAA859Hu04 甘露聚糖结合凝集素关联丝氨酸蛋白酶2(MASP2)多克隆抗体 Polyclonal Antibody to Mannose Associated Serine Protease 2 (MASP2)

PAA859Hu03 甘露聚糖结合凝集素关联丝氨酸蛋白酶2(MASP2)多克隆抗体 Polyclonal Antibody to Mannose Associated Serine Protease 2 (MASP2)

PAA859Hu02 甘露聚糖结合凝集素关联丝氨酸蛋白酶2(MASP2)多克隆抗体 Polyclonal Antibody to Mannose Associated Serine Protease 2 (MASP2)

PAB895Hu01 甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)多克隆抗体 Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1)

PAB895Mu01 甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)多克隆抗体 Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1)

PAB895Mu02 微原纤维胶原相关蛋白1抗体甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)多克隆抗体 Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1)

PAD386Hu01 乳腺丝抑蛋白(Maspin)多克隆抗体 Polyclonal Antibody to Mammary Serine Protease Inhibitor (Maspin)

PAA253Hu01 麦芽糖酶糖化酶(MGA)多克隆抗体 Polyclonal Antibody to Maltase Glucoamylase, Intestinal (MGA)

PAA597Ge01 丙二醛(MDA)多克隆抗体 Polyclonal Antibody to Malondialdehyde (MDA)