肌球蛋白轻链9抗体

肌球蛋白轻链9抗体 

『别名』20 kDa myosin light chain; Human 20kDa myosin light chain (MLC2) mRNA complete cds; LC20; MGC3505; MLC 2; MLC-2C; MLC2; MLY 9; MRLC1; MYL9; MYL9_HUMAN; Myosin light chain 9 regulatory; Myosin light polypeptide 9 regulatory; Myosin regulatory light chain 2; Myosin regulatory light chain 2 smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light polypeptide 9; Myosin RLC; Myosin vascular smooth muscle light chain 2; MYRL2;肌球蛋白轻链9抗体 OTTHUMP00000030857; smooth muscle isoform. 

『浓度』1mg/1ml 

『规格』0.1ml/100μg  0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

肌球蛋白轻链9抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

肌球蛋白轻链9抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAA510Mu01 蛋白激酶D2(PKD2)多克隆抗体 Polyclonal Antibody to Protein Kinase D2 (PKD2)

PAA508Hu01 蛋白激酶D1(PRKD1)多克隆抗体 Polyclonal Antibody to Protein Kinase D1 (PKD1)

PAA508Mu01 蛋白激酶D1(PRKD1)多克隆抗体 Polyclonal Antibody to Protein Kinase D1 (PKD1)

PAB380Hu01 蛋白激酶Cζ(PKCζ)多克隆抗体 Polyclonal Antibody to Protein Kinase C Zeta (PKCz)

PAB380Ra01 蛋白激酶Cζ(PKCζ)多克隆抗体 Polyclonal Antibody to Protein Kinase C Zeta (PKCz)

PAB380Mu01 蛋白激酶Cζ(PKCζ)多克隆抗体 Polyclonal Antibody to Protein Kinase C Zeta (PKCz)

PAA494Hu01 蛋白激酶Cθ(PKCθ)多克隆抗体 Polyclonal Antibody to Protein Kinase C Theta (PKCq)

PAA419Ra01 蛋白激酶Cγ(PKCγ)多克隆抗体 Polyclonal Antibody to Protein Kinase C Gamma (PKCg)

PAA233Bo01 蛋白激酶Cη(PKCη)多克隆抗体 Polyclonal Antibody to Protein Kinase C Eta (PKCh)

PAA233Hu01 蛋白激酶Cη(PKCη)多克隆抗体 Polyclonal Antibody to Protein Kinase C Eta (PKCh)

PAA233Mu01 蛋白激酶Cη(PKCη)多克隆抗体 Polyclonal Antibody to Protein Kinase C Eta (PKCh)

PAA233Ra01 蛋白激酶Cη(PKCη)多克隆抗体 Polyclonal Antibody to Protein Kinase C Eta (PKCh)

PAA439Hu01 蛋白激酶Cε(PKCε)多克隆抗体 Polyclonal Antibody to Protein Kinase C Epsilon (PKCe)

PAA439Mu01 蛋白激酶Cε(PKCε)多克隆抗体 Polyclonal Antibody to Protein Kinase C Epsilon (PKCe)

PAA439Mu02 蛋白激酶Cε(PKCε)多克隆抗体 Polyclonal Antibody to Protein Kinase C Epsilon (PKCe)

PAA439Ra01 蛋白激酶Cε(PKCε)多克隆抗体 Polyclonal Antibody to Protein Kinase C Epsilon (PKCe)

PAA433Hu01 蛋白激酶Cδ(PKCδ)多克隆抗体 Polyclonal Antibody to Protein Kinase C Delta (PKCd)

PAA433Mu01 蛋白激酶Cδ(PKCδ)多克隆抗体 Polyclonal Antibody to Protein Kinase C Delta (PKCd)

PAA433Ra01 蛋白激酶Cδ(PKCδ)多克隆抗体 Polyclonal Antibody to Protein Kinase C Delta (PKCd)

PAA433Mu02 蛋白激酶Cδ(PKCδ)多克隆抗体 Polyclonal Antibody to Protein Kinase C Delta (PKCd)

PAB045Hu01 蛋白激酶Cβ1(PKCβ1)多克隆抗体 Polyclonal Antibody to Protein Kinase C Beta 1 (PKCb1)

PAB045Ra01 蛋白激酶Cβ1(PKCβ1)多克隆抗体 Polyclonal Antibody to Protein Kinase C Beta 1 (PKCb1)

PAB045Rb51 蛋白激酶Cβ1(PKCβ1)多克隆抗体 Polyclonal Antibody to Protein Kinase C Beta 1 (PKCb1)

PAB918Mu01 蛋白激酶Cα(PKCα)多克隆抗体 Polyclonal Antibody to Protein Kinase C Alpha (PKCa)

PAB918Ra01 蛋白激酶Cα(PKCα)多克隆抗体 Polyclonal Antibody to Protein Kinase C Alpha (PKCa)

PAB918Hu01 蛋白激酶Cα(PKCα)多克隆抗体 Polyclonal Antibody to Protein Kinase C Alpha (PKCa)

PAB918Mu02 蛋白激酶Cα(PKCα)多克隆抗体 Polyclonal Antibody to Protein Kinase C Alpha (PKCa)

PAA382Hu01 蛋白激酶Bγ(PKBγ)多克隆抗体 Polyclonal Antibody to Protein Kinase B Gamma (PKBg)

PAB719Hu01 蛋白激酶Bβ(PKBβ)多克隆抗体 Polyclonal Antibody to Protein Kinase B Beta (PKBb)

PAB719Ra01 蛋白激酶Bβ(PKBβ)多克隆抗体 Polyclonal Antibody to Protein Kinase B Beta (PKBb)

PAC231Hu01 蛋白激酶Bα(PKBα)多克隆抗体 Polyclonal Antibody to Protein Kinase B Alpha (PKBa)

PAC231Mu01 蛋白激酶Bα(PKBα)多克隆抗体 Polyclonal Antibody to Protein Kinase B Alpha (PKBa)

PAC231Mu02 蛋白激酶Bα(PKBα)多克隆抗体 Polyclonal Antibody to Protein Kinase B Alpha (PKBa)

PAA931Mu01 蛋白激酶Cα相互作用蛋白1(PICK1)多克隆抗体 Polyclonal Antibody to Protein Interacting With Protein Kinase C Alpha 1 (PICK1)

PAA931Mu02 蛋白激酶Cα相互作用蛋白1(PICK1)多克隆抗体 Polyclonal Antibody to Protein Interacting With Protein Kinase C Alpha 1 (PICK1)

PAE590Hu01 激活STAT蛋白抑制因子3(PIAS3)多克隆抗体 Polyclonal Antibody to Protein Inhibitor Of Activated STAT 3 (PIAS3)

PAB736Hu01 激活STAT蛋白抑制因子1(PIAS1)多克隆抗体 Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1)

PAD776Hu01 蛋白二硫化物异构酶A6(PDIA6)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase A6 (PDIA6)

PAD775Hu01 蛋白二硫化物异构酶A5(PDIA5)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase A5 (PDIA5)

PAD774Hu01 蛋白二硫化物异构酶A4(PDIA4)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase A4 (PDIA4)

PAD774Mu01 蛋白二硫化物异构酶A4(PDIA4)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase A4 (PDIA4)

PAD774Mu02 蛋白二硫化物异构酶A4(PDIA4)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase A4 (PDIA4)

PAB497Hu01 蛋白二硫化物异构酶A3(PDIA3)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase A3 (PDIA3)

PAB498Hu01 蛋白二硫化物异构酶A2(PDIA2)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase A2 (PDIA2)

PAB498Mu01 蛋白二硫化物异构酶A2(PDIA2)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase A2 (PDIA2)

PAB498Mu02 蛋白二硫化物异构酶A2(PDIA2)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase A2 (PDIA2)

PAB061Hu01 蛋白二硫键异构酶(PDI)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase (PDI)

PAB061Ra01 蛋白二硫键异构酶(PDI)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase (PDI)

PAB061Mu01 蛋白二硫键异构酶(PDI)多克隆抗体 Polyclonal Antibody to Protein Disulfide Isomerase (PDI)

PAA022Bo01 血管内皮细胞蛋白C受体(PROCR)多克隆抗体 Polyclonal Antibody to Protein C Receptor, Endothelial (PROCR)

PAA022Ga01 血管内皮细胞蛋白C受体(PROCR)多克隆抗体 Polyclonal Antibody to Protein C Receptor, Endothelial (PROCR)

PAA022Hu01 血管内皮细胞蛋白C受体(PROCR)多克隆抗体 Polyclonal Antibody to Protein C Receptor, Endothelial (PROCR)

PAA022Mu01 血管内皮细胞蛋白C受体(PROCR)多克隆抗体 Polyclonal Antibody to Protein C Receptor, Endothelial (PROCR)

PAA022Po01 血管内皮细胞蛋白C受体(PROCR)多克隆抗体 Polyclonal Antibody to Protein C Receptor, Endothelial 肌球蛋白轻链9抗体(PROCR)

PAA022Ra01 血管内皮细胞蛋白C受体(PROCR)多克隆抗体 Polyclonal Antibody to Protein C Receptor, Endothelial (PROCR)

PAA022Mu02 血管内皮细胞蛋白C受体(PROCR)多克隆抗体 Polyclonal Antibody to Protein C Receptor, Endothelial (PROCR)

PAD393Hu01 蛋白C抑制因子(PCI)多克隆抗体 Polyclonal Antibody to Protein C Inhibitor (PCI)

PAA734Hu01 蛋白C(PROC)多克隆抗体 Polyclonal Antibody to Protein C (PROC)