磷酸化丝裂原活化蛋白激酶MAP4K4抗体

磷酸化丝裂原活化蛋白激酶MAP4K4抗体 

『别名』MAP4K4(phospho S631); Hepatocyte progenitor kinase like/germinal center kinase like kinase; HGK; HPK/GCK like kinase; HPK/GCK like kinase HGK; KIAA0687; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK. 

『浓度』磷酸化丝裂原活化蛋白激酶MAP4K4抗体1mg/1ml 

『规格』0.1ml/100μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

磷酸化丝裂原活化蛋白激酶MAP4K4抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

磷酸化丝裂原活化蛋白激酶MAP4K4抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAB769Ra01 S100钙结合蛋白A6(S100A6)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A6 (S100A6)

PAB769Mu02 S100钙结合蛋白A6(S100A6)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A6 (S100A6)

PAC021Hu01 S100钙结合蛋白A5(S100A5)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A5 (S100A5)

PAC021Ra01 S100钙结合蛋白A5(S100A5)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A5 (S100A5)

PAC020Ra01 S100钙结合蛋白A4(S100A4)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A4 (S100A4)

PAC020Hu01 S100钙结合蛋白A4(S100A4)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A4 (S100A4)

PAC020Mu01 S100钙结合蛋白A4(S100A4)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A4 (S100A4)

PAC020Bo01 S100钙结合蛋白A4(S100A4)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A4 (S100A4)

PAC020Mu02 S100钙结合蛋白A4(S100A4)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A4 (S100A4)

PAC015Hu01 S100钙结合蛋白A3(S100A3)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A3 (S100A3)

PAC015Ra01 S100钙结合蛋白A3(S100A3)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A3 (S100A3)

PAC009Hu01 S100钙结合蛋白A2(S100A2)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A2 (S100A2)

PAC009Hu02 S100钙结合蛋白A2(S100A2)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A2 (S100A2)

PAC009Bo01 S100钙结合蛋白A2(S100A2)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A2 (S100A2)

PAD157Hu01 S100钙结合蛋白A16(S100A16)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A16 (S100A16)

PAD157Ra01 S100钙结合蛋白A16(S100A16)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A16 (S100A16)

PAQ981Hu01 S100钙结合蛋白A15(S100A15)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A15 (S100A15)

PAD158Hu01 S100钙结合蛋白A14(S100A14)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A14 (S100A14)

PAB078Hu01 S100钙结合蛋白A13(S100A13)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A13 (S100A13)

PAB080Hu01 S100钙结合蛋白A12(S100A12)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A12 (S100A12)

PAA568Hu01 S100钙结合蛋白A11(S100A11)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11)

PAA568Mu01 S100钙结合蛋白A11(S100A11)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11)

PAA568Ra01 S100钙结合蛋白A11(S100A11)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11)

PAA568Rb52 S100钙结合蛋白A11(S100A11)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11)

PAA568Rb51 S100钙结合蛋白A11(S100A11)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11)

PAA568Po01 S100钙结合蛋白A11(S100A11)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11)

PAA568Mu02 S100钙结合蛋白A11(S100A11)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11)

PAA568Ga01 S100钙结合蛋白A11(S100A11)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11)

PAC046Bo01 S100钙结合蛋白A10(S100A10)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A10 (S100A10)

PAC046Hu01 S100钙结合蛋白A10(S100A10)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A10 (S100A10)

PAC046Ra01 S100钙结合蛋白A10(S100A10)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A10 (S100A10)

PAC046Mu01 S100钙结合蛋白A10(S100A10)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A10 (S100A10)

PAC046Mu02 S100钙结合蛋白A10(S100A10)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A10 (S100A10)

PAA012Bo01 S100钙结合蛋白(S100)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein (S100)

PAA012Hu01 S100钙结合蛋白(S100)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein (S100)

PAA012Mu01 S100钙结合蛋白(S100)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein (S100)

PAA012Ra01 S100钙结合蛋白(S100)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein (S100)

PAA012Mu02 S100钙结合蛋白(S100)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein (S100)

PAB911Hu01 S抗原(SAG)多克隆抗体 Polyclonal Antibody to S Antigen (SAG)

PAB911Mu01 S抗原(SAG)多克隆抗体 Polyclonal Antibody to S Antigen (SAG)

PAD024Hu01 心肌型兰尼定受体2(RYR2)多克隆抗体 Polyclonal Antibody to Ryanodine Receptor 2, Cardiac (RYR2)

PAC773Mu01 兰尼定受体1(RYR1)多克隆抗体 Polyclonal Antibody to Ryanodine Receptor 1, Skeletal (RYR1)

PAC773Ra01 兰尼定受体1(RYR1)多克隆抗体 Polyclonal Antibody to Ryanodine Receptor 1, Skeletal (RYR1)

PAC773Hu01 兰尼定受体1(RYR1)多克隆抗体 Polyclonal Antibody to Ryanodine Receptor 1, Skeletal (RYR1)

PAC773Mu02 兰尼定受体1(RYR1)多克隆抗体 Polyclonal Antibody to Ryanodine Receptor 1, Skeletal (RYR1)

PAB011Hu01 Runt相关转录因子2(RUNX2)多克隆抗体 Polyclonal Antibody to Runt Related Transcription Factor 2 (RUNX2)

PAB011Mu01 Runt相关转录因子2(RUNX2)多克隆抗体 Polyclonal Antibody to Runt Related Transcription Factor 2 (RUNX2)

PAB011Mu02 Runt相关转录因子2(RUNX2)多克隆抗体 Polyclonal Antibody to Runt Related Transcription Factor 2 (RUNX2)

PAM173Hu01 R-脊椎蛋白3(RSPO3)多克隆抗体 Polyclonal Antibody to R-Spondin 3 (RSPO3)

PAM171Hu01 R-脊椎蛋白1(RSPO1)多克隆抗体 Polyclonal Antibody to R-Spondin 1 (RSPO1)

PAM171Mu01 R-脊椎蛋白1(RSPO1)多克隆抗体 Polyclonal Antibody to R-Spondin 1 (RSPO1)

PAM171Ra01 R-脊椎蛋白1(RSPO1)多克隆抗体 Polyclonal Antibody to R-Spondin 1 (RSPO1)

PAM171Ra02 R-脊椎蛋白1(RSPO1)多克隆抗体 Polyclonal Antibody to R-Spondin 1 (RSPO1)

PAM171Mu02 R-脊椎蛋白1(RSPO1)多克隆抗体 Polyclonal Antibody to R-Spondin 1 (RSPO1)

PAM134Hu01 RNA核酸外切酶2同源物(REXO2)多克隆抗体 Polyclonal Antibody to RNA Exonuclease 2 Homolog (REXO2)

PAG647Hu01 RNA结合基元蛋白38磷酸化丝裂原活化蛋白激酶MAP4K4抗体(RBM38)多克隆抗体 Polyclonal Antibody to RNA Binding Motif Protein 38 (RBM38)

PAG653Hu01 RNA结合基元蛋白3(RBM3)多克隆抗体 Polyclonal Antibody to RNA Binding Motif Protein 3 (RBM3)

PAQ971Hu01 RNA结合基元蛋白24(RBM24)多克隆抗体 Polyclonal Antibody to RNA Binding Motif Protein 24 (RBM24)

PAN021Hu01 RNA结合基元蛋白20(RBM20)多克隆抗体 Polyclonal Antibody to RNA Binding Motif Protein 20 (RBM20)