磷酸化丝裂原活化蛋白激酶MAP4K4抗体

磷酸化丝裂原活化蛋白激酶MAP4K4抗体 

『别名』MAP4K4(phospho S629); Hepatocyte progenitor kinase like/germinal center kinase like kinase; HGK; HPK/GCK like kinase; HPK/GCK like kinase HGK; KIAA0687; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK. 

『浓度』磷酸化丝裂原活化蛋白激酶MAP4K4抗体1mg/1ml 

『规格』0.1ml/100μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

磷酸化丝裂原活化蛋白激酶MAP4K4抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

磷酸化丝裂原活化蛋白激酶MAP4K4抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAG850Hu01 促泌素(SCGN)多克隆抗体 Polyclonal Antibody to Secretagogin (SCGN)

PAG850Mu01 促泌素(SCGN)多克隆抗体 Polyclonal Antibody to Secretagogin (SCGN)

PAG850Ra01 促泌素(SCGN)多克隆抗体 Polyclonal Antibody to Secretagogin (SCGN)

PAG850Mu02 促泌素(SCGN)多克隆抗体 Polyclonal Antibody to Secretagogin (SCGN)

PAB575Hu01 二级淋巴组织趋化因子(SLC)多克隆抗体 Polyclonal Antibody to Secondary Lymphoid Tissue Chemokine (SLC)

PAB575Mu01 二级淋巴组织趋化因子(SLC)多克隆抗体 Polyclonal Antibody to Secondary Lymphoid Tissue Chemokine (SLC)

PAB575Ra01 二级淋巴组织趋化因子(SLC)多克隆抗体 Polyclonal Antibody to Secondary Lymphoid Tissue Chemokine (SLC)

PAB575Mu02 二级淋巴组织趋化因子(SLC)多克隆抗体 Polyclonal Antibody to Secondary Lymphoid Tissue Chemokine (SLC)

PAM192Hu01 SCO细胞色素氧化酶缺陷同源物2(SCO2)多克隆抗体 Polyclonal Antibody to SCO Cytochrome Oxidase Deficient Homolog 2 (SCO2)

PAF900Hu01 含硬化蛋白域蛋白1(SOSTDC1)多克隆抗体 Polyclonal Antibody to Sclerostin Domain Containing Protein 1 (SOSTDC1)

PAF900Mu01 含硬化蛋白域蛋白1(SOSTDC1)多克隆抗体 Polyclonal Antibody to Sclerostin Domain Containing Protein 1 (SOSTDC1)

PAF900Mu02 含硬化蛋白域蛋白1(SOSTDC1)多克隆抗体 Polyclonal Antibody to Sclerostin Domain Containing Protein 1 (SOSTDC1)

PAC864Hu01 硬骨素(SOST)多克隆抗体 Polyclonal Antibody to Sclerostin (SOST)

PAC864Mu01 硬骨素(SOST)多克隆抗体 Polyclonal Antibody to Sclerostin (SOST)

PAC864Ra01 硬骨素(SOST)多克隆抗体 Polyclonal Antibody to Sclerostin (SOST)

PAC864Mu02 硬骨素(SOST)多克隆抗体 Polyclonal Antibody to Sclerostin (SOST)

PAT801Hu01 Scleraxis同源物B(SCXB)多克隆抗体 Polyclonal Antibody to Scleraxis Homolog B (SCXB)

PAN330Hu01 Scleraxis同源物A(SCXA)多克隆抗体 Polyclonal Antibody to Scleraxis Homolog A (SCXA)

PAR803Hu01 Schlafen家族成员5(SLFN5)多克隆抗体 Polyclonal Antibody to Schlafen Family Member 5 (SLFN5)

PAB257Hu01 D类清道夫受体1(SCARD1)多克隆抗体 Polyclonal Antibody to Scavenger Receptor Class D Member 1 (SCARD1)

PAB257Mu01 D类清道夫受体1(SCARD1)多克隆抗体 Polyclonal Antibody to Scavenger Receptor Class D Member 1 (SCARD1)

PAH194Hu01 B类清道夫受体1(SCARB1)多克隆抗体 Polyclonal Antibody to Scavenger Receptor Class B Member 1 (SCARB1)

PAH194Ra01 B类清道夫受体1(SCARB1)多克隆抗体 Polyclonal Antibody to Scavenger Receptor Class B Member 1 (SCARB1)

PAH195Hu01 A类清道夫受体5(SCARA5)多克隆抗体 Polyclonal Antibody to Scavenger Receptor Class A Member 5 (SCARA5)

PAH193Mu01 A类清道夫受体3(SCARA3)多克隆抗体 Polyclonal Antibody to Scavenger Receptor Class A Member 3 (SCARA3)

PAH193Mu02 A类清道夫受体3(SCARA3)多克隆抗体 Polyclonal Antibody to Scavenger Receptor Class A Member 3 (SCARA3)

PAT081Hu01 含SCAN域蛋白3(SCAND3)多克隆抗体 Polyclonal Antibody to SCAN Domain Containing Protein 3 (SCAND3)

PAK006Hu01 SATB同源框蛋白1(SATB1)多克隆抗体 Polyclonal Antibody to SATB Homeobox Protein 1 (SATB1)

PAC848Hu01 肌脂蛋白(SLN)多克隆抗体 Polyclonal Antibody to Sarcolipin (SLN)

PAC848Ra01 肌脂蛋白(SLN)多克隆抗体 Polyclonal Antibody to Sarcolipin (SLN)

PAE226Hu01 肌糖δ(SGCδ)多克隆抗体 Polyclonal Antibody to Sarcoglycan Delta (SGCd)

PAC026Hu01 Salusin肽β(SALβ)多克隆抗体 Polyclonal Antibody to Salusin Beta (SALb)

PAB892Hu01 Salusin肽α(SALα)多克隆抗体 Polyclonal Antibody to Salusin Alpha (SALa)

PAM257Hu01 盐诱导激酶2(SIK2)多克隆抗体 Polyclonal Antibody to Salt Inducible Kinase 2 (SIK2)

PAM257Mu01 盐诱导激酶2(SIK2)多克隆抗体 Polyclonal Antibody to Salt Inducible Kinase 2 (SIK2)

PAE158Hu01 Saitohin蛋白(STH)多克隆抗体 Polyclonal Antibody to Saitohin (STH)

PAG414Ge01 S-腺苷蛋氨酸(SAM)多克隆抗体 Polyclonal Antibody to S-Adenosyl Methionine (SAM)

PAC054Hu01 S100钙结合蛋白P(S100P)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein P (S100P)

PAA567Hu01 S100钙结合蛋白B(S100B)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein B (S100B)

PAA567Mu01 S100钙结合蛋白B(S100B)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein B (S100B)

PAA567Ra01 S100钙结合蛋白B(S100B)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein B (S100B)

PAA567Rb52 S100钙结合蛋白B(S100B)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein B (S100B)

PAA567Rb51 S100钙结合蛋白B(S100B)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein B (S100B)

PAA567Mu02 S100钙结合蛋白B(S100B)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein B (S100B)

PAA567Bo01 S100钙结合蛋白B(S100B)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein B (S100B)

PAB793Bo01 S100钙结合蛋白A9(S100A9)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9)

PAB793Hu01 S100钙结合蛋白A9(S100A9)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9)

PAB793Mu01 S100钙结合蛋白A9(S100A9)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9)

PAB793Ra01 S100钙结合蛋白A9(S100A9)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9)

PAB793Mu02 S100钙结合蛋白A9(S100A9)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9)

PAB792Bo01 S100钙结合蛋白A8(S100A8)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)

PAB792Hu01 S100钙结合蛋白A8(S100A8)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)

PAB792Mu01 S100钙结合蛋白A8(S100A8)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)

PAB792Ra01 S100钙结合蛋白A8(S100A8)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)

PAB792Mu02 S100钙结合蛋白A8(S100A8)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)

PAC035Hu01 S100钙结合蛋白A7(S100A7)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A7 磷酸化丝裂原活化蛋白激酶MAP4K4抗体(S100A7)

PAC035Mu01 S100钙结合蛋白A7(S100A7)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A7 (S100A7)

PAC035Mu02 S100钙结合蛋白A7(S100A7)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A7 (S100A7)

PAC035Bo01 S100钙结合蛋白A7(S100A7)多克隆抗体 Polyclonal Antibody to S100 Calcium Binding Protein A7 (S100A7)