?单羧酸转运蛋白-1抗体
『别名』monocarboxylate transporter 1; Malignant T cell amplified sequence 1; MCT 1; MCT1; MCTS 1; MCTS1; Oncogene MCT 1; Oncogene MCT1; SLC16A1; FLJ36745; HHF7; MCT; MGC44475; monocarboxylate transporter; Monocarboxylate transporter isoform 1; Monocarboxylic acid transporter 1; SLC16A1; SLC16A1 protein; Solute carrier family 16 (monocarboxylic acid transporters) member 1; Solute carrier family 16 member 1 (monocarboxylic acid transporter 1); Solute carrier family 16 member 1; MOT1_HUMAN.
『浓度』单羧酸转运蛋白-1抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
单羧酸转运蛋白-1抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
单羧酸转运蛋白-1抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAG962Mu02 翻译调节肿瘤蛋白1(TPT1)多克隆抗体 Polyclonal Antibody to Tumor Protein, Translationally Controlled 1 (TPT1)
PAC805Hu01 肿廇蛋白P63(TP63)多克隆抗体 Polyclonal Antibody to Tumor Protein P63 (TP63)
PAC805Mu01 肿廇蛋白P63(TP63)多克隆抗体 Polyclonal Antibody to Tumor Protein P63 (TP63)
PAC805Mu02 肿廇蛋白P63(TP63)多克隆抗体 Polyclonal Antibody to Tumor Protein P63 (TP63)
PAH009Hu01 肿瘤蛋白p53结合蛋白1(TP53BP1)多克隆抗体 Polyclonal Antibody to Tumor Protein p53 Binding Protein 1 (TP53BP1)
PAA928Hu01 肿瘤蛋白p53(TP53)多克隆抗体 Polyclonal Antibody to Tumor Protein p53 (TP53)
PAA928Mu01 肿瘤蛋白p53(TP53)多克隆抗体 Polyclonal Antibody to Tumor Protein p53 (TP53)
PAA928Ra01 肿瘤蛋白p53(TP53)多克隆抗体 Polyclonal Antibody to Tumor Protein p53 (TP53)
PAA928Mu02 肿瘤蛋白p53(TP53)多克隆抗体 Polyclonal Antibody to Tumor Protein p53 (TP53)
PAA928Hu02 肿瘤蛋白p53(TP53)多克隆抗体 Polyclonal Antibody to Tumor Protein p53 (TP53)
PAA139Ca01 肿瘤坏死因子相关凋亡诱导配体(TRAIL)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
PAA139Hu01 肿瘤坏死因子相关凋亡诱导配体(TRAIL)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
PAA139Mu01 肿瘤坏死因子相关凋亡诱导配体(TRAIL)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
PAA139Ra01 肿瘤坏死因子相关凋亡诱导配体(TRAIL)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
PAA139Mu02 肿瘤坏死因子相关凋亡诱导配体(TRAIL)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
PAB527Hu01 肿瘤坏死因子受体超家族成员9(TNFRSF9)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9)
PAB043Hu01 肿瘤坏死因子受体超家族成员7(TNFRSF7)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)
PAB249Mu01 肿瘤坏死因子受体超家族成员5(TNFRSF5)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5)
PAB249Mu02 肿瘤坏死因子受体超家族成员5(TNFRSF5)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5)
PAB249Ra01 肿瘤坏死因子受体超家族成员5(TNFRSF5)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5)
PAB249Hu01 肿瘤坏死因子受体超家族成员5(TNFRSF5)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5)
PAB519Mu01 肿瘤坏死因子受体超家族成员4(TNFRSF4)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4)
PAB519Mu02 肿瘤坏死因子受体超家族成员4(TNFRSF4)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4)
PAD929Hu01 肿瘤坏死因子受体超家族成员21(TNFRSF21)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21)
PAD929Mu01 肿瘤坏死因子受体超家族成员21(TNFRSF21)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21)
PAB504Ra01 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)
PAB504Mu01 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)
PAB504Hu01 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)
PAB499Ra01 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)
PAB499Hu01 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)
PAB499Mu01 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)
PAB499Ra02 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)
PAB499Hu02 肿瘤坏死因子受体超家族成员1A(TNFRSF1A)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)
PAD925Mu01 肿瘤坏死因子受体超家族成员19样蛋白(TNFRSF19L)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 19 Like Protein (TNFRSF19L)
PAD925Mu02 肿瘤坏死因子受体超家族成员19样蛋白(TNFRSF19L)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 19 Like Protein (TNFRSF19L)
PAD928Mu01 肿瘤坏死因子受体超家族成员19(TNFRSF19)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19)
PAD928Hu01 肿瘤坏死因子受体超家族成员19(TNFRSF19)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19)
PAA790Mu01 肿瘤坏死因子受体超家族成员17(TNFRSF17)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17)
PAA790Mu02 肿瘤坏死因子受体超家族成员17(TNFRSF17)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17)
PAD926Mu01 肿瘤坏死因子受体超家族成员14(TNFRSF14)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14)
PAD926Hu01 肿瘤坏死因子受体超家族成员14(TNFRSF14)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14)
PAA813Hu01 肿瘤坏死因子受体超家族成员12A(TNFRSF12A)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)
PAA818Mu01 肿瘤坏死因子受体超家族成员10B(TNFRSF10B)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B)
PAA818Mu02 肿瘤坏死因子受体超家族成员10B(TNFRSF10B)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B)
PAA818Ra01 肿瘤坏死因子受体超家族成员10B(TNFRSF10B)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B)
PAA818Hu01 肿瘤坏死因子受体超家族成员10B(TNFRSF10B)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B)
PAA136Mu01 肿瘤坏死因子受体超家族成员10A(TNFRSF10A)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10A (TNFRSF10A)
PAA136Mu02 肿瘤坏死因子受体超家族成员10A(TNFRSF10A)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 10A (TNFRSF10A)
PAD934Hu01 肿瘤坏死因子配体超家族成员9(TNFSF9)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9)
PAD934Mu01 肿瘤坏死因子配体超家族成员9(TNFSF9)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9)
PAB251Hu01 肿瘤坏死因子配体超家族成员7(TNFSF7)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7)
PAB251Mu01 肿瘤坏死因子配体超家族成员7(TNFSF7)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7)
PAA850Hu01 肿瘤坏死因子配体超家族成员4(TNFSF4)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4)
PAA827Mu01 单羧酸转运蛋白-1抗体肿瘤坏死因子配体超家族成员14(TNFSF14)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14)
PAA827Hu01 肿瘤坏死因子配体超家族成员14(TNFSF14)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14)
PAA827Ra01 肿瘤坏死因子配体超家族成员14(TNFSF14)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14)