多药耐药相关蛋白3抗体
『别名』ABC 31; ABC31; ABCC 3; ABCC3; ATP binding cassette sub family C (CFTR/MRP) member 3; ATP binding cassette sub family C; ATP binding cassette sub family C member 3; Canalicular multispecific organic anion transporter 2; Canicular multispecific organic anion transporter; CMOAT 2; CMOAT2; EST90757; MLP 2; MLP2; MOAT D; MOATD; MRP 3; Multi specific organic anion transporter D; Multidrug resistance associated protein 3; Multidrug resistance associated protein; Multispecific organic anion tranporter D.
『浓度』多药耐药相关蛋白3抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
多药耐药相关蛋白3抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
多药耐药相关蛋白3抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA146Mu01 血管内皮生长因子D(VEGFD)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD)
PAA146Ra01 血管内皮生长因子D(VEGFD)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD)
PAA146Bo01 血管内皮生长因子D(VEGFD)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD)
PAA146Mu02 血管内皮生长因子D(VEGFD)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD)
PAA145Hu01 血管内皮生长因子C(VEGFC)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC)
PAA145Mu01 血管内皮生长因子C(VEGFC)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC)
PAA145Ra01 血管内皮生长因子C(VEGFC)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC)
PAA145Bo01 血管内皮生长因子C(VEGFC)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC)
PAA145Mu02 血管内皮生长因子C(VEGFC)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC)
PAA144Hu01 血管内皮生长因子B(VEGFB)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
PAA144Mu01 血管内皮生长因子B(VEGFB)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
PAA144Ra01 血管内皮生长因子B(VEGFB)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
PAA144Bo01 血管内皮生长因子B(VEGFB)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
PAA144Mu02 血管内皮生长因子B(VEGFB)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
PAA143Hu01 血管内皮生长因子A(VEGFA)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)
PAA143Mu01 血管内皮生长因子A(VEGFA)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)
PAA143Ra01 血管内皮生长因子A(VEGFA)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)
PAA143Rb51 血管内皮生长因子A(VEGFA)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)
PAA143Ca01 血管内皮生长因子A(VEGFA)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)
PAA143Eq01 血管内皮生长因子A(VEGFA)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)
PAA143Ga01 血管内皮生长因子A(VEGFA)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)
PAA143Po01 血管内皮生长因子A(VEGFA)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)
PAA143Bo01 血管内皮生长因子A(VEGFA)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)
PAS415Hu01 血管内皮生长因子145(VEGF145)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor 145 (VEGF145)
PAS415Mu01 血管内皮生长因子145(VEGF145)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor 145 (VEGF145)
PAB851Hu01 血管内皮生长因子121(VEGF121)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor 121 (VEGF121)
PAB851Mu01 血管内皮生长因子121(VEGF121)多克隆抗体 Polyclonal Antibody to Vascular Endothelial Growth Factor 121 (VEGF121)
PAA547Hu01 血管细胞粘附分子1(VCAM1)多克隆抗体 Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
PAA547Mu01 血管细胞粘附分子1(VCAM1)多克隆抗体 Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
PAA547Ra01 血管细胞粘附分子1(VCAM1)多克隆抗体 Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
PAA547Rb52 血管细胞粘附分子1(VCAM1)多克隆抗体 Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
PAA547Si01 血管细胞粘附分子1(VCAM1)多克隆抗体 Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
PAA547Rb51 血管细胞粘附分子1(VCAM1)多克隆抗体 Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
PAA547Mu02 血管细胞粘附分子1(VCAM1)多克隆抗体 Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
PAA547Ca01 血管细胞粘附分子1(VCAM1)多克隆抗体 Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
PAA547Po01 血管细胞粘附分子1(VCAM1)多克隆抗体 Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
PAD870Hu01 血管非炎性蛋白3(VNN3)多克隆抗体 Polyclonal Antibody to Vanin 3 (VNN3)
PAD870Mu01 血管非炎性蛋白3(VNN3)多克隆抗体 Polyclonal Antibody to Vanin 3 (VNN3)
PAD870Mu02 血管非炎性蛋白3(VNN3)多克隆抗体 Polyclonal Antibody to Vanin 3 (VNN3)
PAC598Hu01 血管非炎性蛋白1(VNN1)多克隆抗体 Polyclonal Antibody to Vanin 1 (VNN1)
PAC598Ra01 血管非炎性蛋白1(VNN1)多克隆抗体 Polyclonal Antibody to Vanin 1 (VNN1)
PAC598Mu01 血管非炎性蛋白1(VNN1)多克隆抗体 Polyclonal Antibody to Vanin 1 (VNN1)
PAC598Hu02 血管非炎性蛋白1(VNN1)多克隆抗体 Polyclonal Antibody to Vanin 1 (VNN1)
PAC598Hu03 血管非炎性蛋白1(VNN1)多克隆抗体 Polyclonal Antibody to Vanin 1 (VNN1)
PAC598Bo01 血管非炎性蛋白1(VNN1)多克隆抗体 Polyclonal Antibody to Vanin 1 (VNN1)
PAP190Mu01 Vang样蛋白1(VANGL1)多克隆抗体 Polyclonal Antibody to Vang Like Protein 1 (VANGL1)
PAC601Hu01 含缬酪肽蛋白(VCP)多克隆抗体 Polyclonal Antibody to Valosin Containing Protein (VCP)
PAC601Ra01 含缬酪肽蛋白(VCP)多克隆抗体 Polyclonal Antibody to Valosin Containing Protein (VCP)
PAN411Mu01 液泡蛋白分拣蛋白13D(VPS13D)多克隆抗体 Polyclonal Antibody to Vacuolar Protein Sorting 13D (VPS13D)
PAN411Mu02 液泡蛋白分拣蛋白13D(VPS13D)多克隆抗体 Polyclonal Antibody to Vacuolar Protein Sorting 13D (VPS13D)
PAC600Hu01 牛痘相关激酶1(VRK1)多克隆抗体 Polyclonal Antibody to Vaccinia Related Kinase 1 (VRK1)
PAL897Hu01 卷曲域锚蛋白重复序列葡萄膜自身抗原(UACA)多克隆抗体 Polyclonal Antibody to Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA)
PAF959Mu01 抗肌萎缩蛋白多药耐药相关蛋白3抗体(UTRN)多克隆抗体 Polyclonal Antibody to Utrophin (UTRN)
PAF959Mu02 抗肌萎缩蛋白(UTRN)多克隆抗体 Polyclonal Antibody to Utrophin (UTRN)
PAL872Hu01 USP6氨基端样蛋白(USP6NL)多克隆抗体 Polyclonal Antibody to USP6 N-Terminal Like Protein (USP6NL)
PAA335Ra01 尾加压素2受体(UTS2R)多克隆抗体 Polyclonal Antibody to Urotensin 2 Receptor (UTS2R)
PAA362Hu01 尾加压素2(UST2)多克隆抗体 Polyclonal Antibody to Urotensin 2 (UST2)