瘦素抗体
『别名』LEP; Leptin Murine Obesity Homolog; Leptin Precursor Obesity Factor; OB; Obese Protein; Obesity; Obesity factor; Obesity homolog mouse; Obesity Murine Homolog Leptin; OBS.
『浓度』瘦素抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
瘦素抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
瘦素抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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ELB1432c 兔信号传导转录激活因子3(STAT3)酶联免疫吸附测定试剂盒 Rabbit STAT3 (Signal Transducer and Activator of Transcription 3) ELISA Kit
ELB0913c 兔信号传导转录激活因子2(STAT2)酶联免疫吸附测定试剂盒 Rabbit STAT2 (Signal Transducer and Activator of Transcription 2) ELISA Kit
ELB0580c 兔信号传导转录激活因子1(STAT1)酶联免疫吸附测定试剂盒 Rabbit STAT1 (Signal Transducer and Activator of Transcription 1) ELISA Kit
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ELB0714c 兔α-胞衬蛋白(SPTAN1)酶联免疫吸附测定试剂盒 Rabbit SPTAN1 (Alpha-Fodrin) ELISA Kit
ELB0187c 兔斑型POZ蛋白(SPOP)酶联免疫吸附测定试剂盒 Rabbit SPOP (Speckle Type POZ Protein) ELISA Kit
ELB1032c 兔表面活性物质关联蛋白J(SPJ)酶联免疫吸附测定试剂盒 Rabbit SPJ (Surfactant Associated Protein J) ELISA Kit
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ELB1985c 兔肺表面活性物质相关蛋白C(SPC)酶联免疫吸附测定试剂盒 Rabbit SPC (Pulmonary Surfactant Associated Protein C) ELISA Kit
ELB0857c 兔肺表面活性物质相关蛋白B(SPB)酶联免疫吸附测定试剂盒 Rabbit SPB (Pulmonary Surfactant Associated Protein B) ELISA Kit
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ELB0507c 兔肺表面活性物质相关蛋白A(SPA)酶联免疫吸附测定试剂盒 Rabbit SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit
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ELB0445c 兔性别决定区Y框蛋白9(SOX9)酶联免疫吸附测定试剂盒 Rabbit SOX9 (Sex Determining Region Y Box Protein 9) ELISA Kit
ELB1469c 兔性别决定区Y框蛋白3(SOX3)酶联免疫吸附测定试剂盒 Rabbit SOX3 (Sex Determining Region Y Box Protein 3) ELISA Kit
ELB0289c 兔性别决定区Y框蛋白2(SOX2)酶联免疫吸附测定试剂盒 Rabbit SOX2 (Sex Determining Region Y Box Protein 2) ELISA Kit
ELB1417c 兔性别决定区Y框蛋白18(SOX18)酶联免疫吸附测定试剂盒 Rabbit SOX18 (Sex Determining Region Y Box Protein 18) ELISA Kit
ELB0351c 兔性别决定区Y框蛋白1(SOX1)酶联免疫吸附测定试剂盒 Rabbit SOX1 (Sex Determining Region Y Box Protein 1) ELISA Kit
ELB2140c 兔胞外超氧化物歧化酶(SOD3)酶联免疫吸附测定试剂盒 Rabbit SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit
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ELB2113c 兔小核糖核蛋白多肽C(SNRPC)酶联免疫吸附测定试剂盒 Rabbit SNRPC (Small Nuclear Ribonucleoprotein Polypeptide C) ELISA Kit
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