马免疫球蛋白E检测试剂盒代测
我们免费代测,品牌供应,我们拥有三千多种酶联免疫吸附测定试剂盒,包含多种种属ELISA试剂盒,待测标本形式多样:有液体及半固体形式。
样本保存:
2-8℃冰箱最多2周
-20到-50℃的冰箱可以放3-6个月
马免疫球蛋白E检测试剂盒代测-50℃一下的冰箱,可以放一年
样本收集:
血清样本用干燥管(红色)或者促凝管(黄色)收集
血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
血浆样本最好是不要用肝素(绿色)抗凝的试管收集
样本处理:
1)血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2)血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3)尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4)细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。马免疫球蛋白E检测试剂盒代测通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5)组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6)标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7)不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
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EIA(B004)-019461 人白介素18(IL-18)ELISA Kit ,Products name: Human IL-18 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-019467 人白介素18受体辅助蛋白(IL18RAP)ELISA Kit ,Products name: Human IL18RAP ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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EIA(B004)-019469 人白介素19(IL-19)ELISA Kit ,Products name: Human IL-19 ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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EIA(B004)-019471 人白介素1B(IL1B)ELISA Kit ,Products name: IL1B ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-019472 人白介素1B(IL-1B)ELISA Kit ,Products name: IL-1B ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-019473 人白介素-1b(IL-1b)ELISA Kit ,Products name: IL-1b ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-019474 人白介素1Rα(IL-1Rα)ELISA Kit ,Products name: Human IL-1Rα ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-019475 人白介素1α(IL1α)ELISA Kit ,Products name: Human IL1α ELISA Kit ,灵敏度: 37.5pg/mL ,检测范围: 62.5-4000pg/mL
EIA(B004)-019476 人白介素1α(IL-1α)ELISA Kit ,Products name: Human Interleukin 1α,IL-1α ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-019477 人白介素1α(IL-1α)ELISA Kit ,Products name: Human Interleukin 1α,IL-1α ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-019478 人白介素1α(IL-1α)ELISA Kit ,Products name: Human IL-1α ELISA Kit ,灵敏度: 11.25pg/mL ,检测范围: 18.75-1200pg/mL
EIA(B004)-019479 人白介素1β (IL-1β)ELISA Kit ,Products name: IL-1β ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B004)-019480 人白介素1β(IL-1B)ELISA Kit ,Products name: Human IL-1B ELISA Kit ,灵敏度: 0.46899999999999997ng/mL ,检测范围: 0.78100000000000003-50ng/mL
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EIA(B004)-019482 人白介素1β(IL1β)ELISA Kit ,Products name: Human IL1β ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-019483 人白介素1β(IL-1β)ELISA Kit ,Products name: IL-1β ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-019484 人白介素1β(IL-1β)ELISA Kit ,Products name: Human Interleukin 1β,IL-1β ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-019486 人白介素1β(IL-1β)ELISA Kit ,Products name: Human IL-1β ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-019489 人白介素1β前体(Pro-IL-1β)ELISA Kit ,Products name: Human Pro-IL-1β ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-019505 人白介素1可溶性受体Ⅰ(IL-1sRⅠ)ELISA Kit ,Products name: Human IL-1sRⅠ ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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