LRRC2蛋白抗体
『别名』Leucine rich repeat containing 2; LRRC2_HUMAN.
『浓度』LRRC2蛋白抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
LRRC2蛋白抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
LRRC2蛋白抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAC605Mu02 X-框结合蛋白1(XBP1)多克隆抗体 Polyclonal Antibody to X-Box Binding Protein 1 (XBP1)
PAC605Ra01 X-框结合蛋白1(XBP1)多克隆抗体 Polyclonal Antibody to X-Box Binding Protein 1 (XBP1)
PAC608Bo01 黄嘌呤脱氢酶(XDH)多克隆抗体 Polyclonal Antibody to Xanthine Dehydrogenase (XDH)
PAC608Hu01 黄嘌呤脱氢酶(XDH)多克隆抗体 Polyclonal Antibody to Xanthine Dehydrogenase (XDH)
PAC608Mu01 黄嘌呤脱氢酶(XDH)多克隆抗体 Polyclonal Antibody to Xanthine Dehydrogenase (XDH)
PAC608Mu02 黄嘌呤脱氢酶(XDH)多克隆抗体 Polyclonal Antibody to Xanthine Dehydrogenase (XDH)
PAC608Ra01 黄嘌呤脱氢酶(XDH)多克隆抗体 Polyclonal Antibody to Xanthine Dehydrogenase (XDH)
PAL087Hu01 含WW域E3泛素蛋白连接酶2(WWP2)多克隆抗体 Polyclonal Antibody to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2)
PAL118Hu01 Wolfram综合征蛋白1(WFS1)多克隆抗体 Polyclonal Antibody to Wolfram Syndrome Protein 1 (WFS1)
PAL118Mu01 Wolfram综合征蛋白1(WFS1)多克隆抗体 Polyclonal Antibody to Wolfram Syndrome Protein 1 (WFS1)
PAL118Ra01 Wolfram综合征蛋白1(WFS1)多克隆抗体 Polyclonal Antibody to Wolfram Syndrome Protein 1 (WFS1)
PAL118Mu02 Wolfram综合征蛋白1(WFS1)多克隆抗体 Polyclonal Antibody to Wolfram Syndrome Protein 1 (WFS1)
PAG894Hu01 WNT1诱导信号通道蛋白2(WISP2)多克隆抗体 Polyclonal Antibody to WNT1 Inducible Signaling Pathway Protein 2 (WISP2)
PAG894Ra01 WNT1诱导信号通道蛋白2(WISP2)多克隆抗体 Polyclonal Antibody to WNT1 Inducible Signaling Pathway Protein 2 (WISP2)
PAG895Hu01 WNT1诱导信号通道蛋白1(WISP1)多克隆抗体 Polyclonal Antibody to WNT1 Inducible Signaling Pathway Protein 1 (WISP1)
PAG895Mu01 WNT1诱导信号通道蛋白1(WISP1)多克隆抗体 Polyclonal Antibody to WNT1 Inducible Signaling Pathway Protein 1 (WISP1)
PAG895Mu02 WNT1诱导信号通道蛋白1(WISP1)多克隆抗体 Polyclonal Antibody to WNT1 Inducible Signaling Pathway Protein 1 (WISP1)
PAL826Hu01 WNT抑制因子1(WIF1)多克隆抗体 Polyclonal Antibody to WNT Inhibitory Factor 1 (WIF1)
PAL826Ra01 WNT抑制因子1(WIF1)多克隆抗体 Polyclonal Antibody to WNT Inhibitory Factor 1 (WIF1)
PAG892Hu01 WNK赖氨酸缺乏蛋白激酶1(WNK1)多克隆抗体 Polyclonal Antibody to WNK Lysine Deficient Protein Kinase 1 (WNK1)
PAE773Hu01 免疫缺陷伴血小板减少综合征蛋白家族成员2(WASF2)多克隆抗体 Polyclonal Antibody to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2)
PAE773Mu01 免疫缺陷伴血小板减少综合征蛋白家族成员2(WASF2)多克隆抗体 Polyclonal Antibody to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2)
PAE773Mu02 免疫缺陷伴血小板减少综合征蛋白家族成员2(WASF2)多克隆抗体 Polyclonal Antibody to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2)
PAP550Hu01 无翅型MMTV整合位点家族成员9B(WNT9B)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 9B (WNT9B)
PAP546Hu01 无翅型MMTV整合位点家族成员7B(WNT7B)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 7B (WNT7B)
PAP547Hu01 无翅型MMTV整合位点家族成员7A(WNT7A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 7A (WNT7A)
PAP122Hu01 无翅型MMTV整合位点家族成员5B(WNT5B)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B)
PAP122Bo01 无翅型MMTV整合位点家族成员5B(WNT5B)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B)
PAP549Hu01 无翅型MMTV整合位点家族成员5A(WNT5A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
PAP549Mu01 无翅型MMTV整合位点家族成员5A(WNT5A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
PAP549Ra01 无翅型MMTV整合位点家族成员5A(WNT5A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
PAP549Mu02 无翅型MMTV整合位点家族成员5A(WNT5A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
PAL817Ra01 无翅型MMTV整合位点家族成员4(WNT4)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 4 (WNT4)
PAL817Hu01 无翅型MMTV整合位点家族成员4(WNT4)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 4 (WNT4)
PAP155Hu01 无翅型MMTV整合位点家族成员3A(WNT3A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
PAP155Ra01 无翅型MMTV整合位点家族成员3A(WNT3A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
PAP155Mu01 无翅型MMTV整合位点家族成员3A(WNT3A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
PAP155Mu02 无翅型MMTV整合位点家族成员3A(WNT3A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
PAP155Bo01 无翅型MMTV整合位点家族成员3A(WNT3A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
PAL818Hu01 无翅型MMTV整合位点家族成员3(WNT3)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3 (WNT3)
PAL818Ra01 无翅型MMTV整合位点家族成员3(WNT3)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3 (WNT3)
PAL820Hu01 无翅型MMTV整合位点家族成员2(WNT2)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 2 (WNT2)
PAP121Hu01 无翅型MMTV整合位点家族成员16(WNT16)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 16 (WNT16)
PAP552Hu01 无翅型MMTV整合位点家族成员11(WNT11)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 11 (WNT11)
PAP553Hu01 无翅型MMTV整合位点家族成员10B(WNT10B)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)
PAP553Mu01 无翅型MMTV整合位点家族成员10B(WNT10B)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)
PAP553Mu02 无翅型MMTV整合位点家族成员10B(WNT10B)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)
PAP358Hu01 无翅型MMTV整合位点家族成员10A(WNT10A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10A (WNT10A)
PAP358Mu01 无翅型MMTV整合位点家族成员10A(WNT10A)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10A (WNT10A)
PAL821Hu01 无翅型MMTV整合位点家族成员1(WNT1)多克隆抗体 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 1 (WNT1)
PAF116Hu01 LRRC2蛋白抗体肾母细胞瘤蛋白1(WT1)多克隆抗体 Polyclonal Antibody to Wilms Tumor Protein (WT1)
PAE552Mu01 WAP四二硫化物核心域蛋白5(WFDC5)多克隆抗体 Polyclonal Antibody to WAP Four Disulfide Core Domain Protein 5 (WFDC5)
PAE552Hu01 WAP四二硫化物核心域蛋白5(WFDC5)多克隆抗体 Polyclonal Antibody to WAP Four Disulfide Core Domain Protein 5 (WFDC5)
PAE552Ra01 WAP四二硫化物核心域蛋白5(WFDC5)多克隆抗体 Polyclonal Antibody to WAP Four Disulfide Core Domain Protein 5 (WFDC5)