富含亮氨酸重复序列Nogo受体反应蛋白4抗体
『别名』Leucine rich repeat and Ig domain containing 4; Leucine rich repeat and immunoglobulin like domain containing nogo receptor interacting protein 4; Leucine rich repeat neuronal protein 6D; LRRN6D; LIGO4_HUMAN.
『浓度』富含亮氨酸重复序列Nogo受体反应蛋白4抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
富含亮氨酸重复序列Nogo受体反应蛋白4抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
富含亮氨酸重复序列Nogo受体反应蛋白4抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
EL2267c猪粒细胞巨噬细胞集落刺激因子(GM-CSF)酶联免疫吸附测定试剂盒 Porcine GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit
EL1209c猪胃粘液素(GM)酶联免疫吸附测定试剂盒 Porcine GM (Gastric Mucin) ELISA Kit
EL1515c猪半乳糖苷酶β(GLβ)酶联免疫吸附测定试剂盒Porcine GLβ (Galactosidase Beta) ELISA Kit
EL0801c猪半乳糖苷酶α(GLα)酶联免疫吸附测定试剂盒 Porcine GLα (Galactosidase Alpha) ELISA Kit
EL2086c猪糖基化依赖的细胞黏附分子(GlyCAM1)酶联免疫吸附测定试剂盒 Porcine GlyCAM1 (Glycosylation Dependent Cell Adhesion Molecule 1) ELISA Kit
EL1786c猪葡萄糖转运蛋白4(GLUT4)酶联免疫吸附测定试剂盒 Porcine GLUT4 (Glucose Transporter 4) ELISA Kit
EL0876c猪葡萄糖转运蛋白3(GLUT3)酶联免疫吸附测定试剂盒 Porcine GLUT3 (Glucose Transporter 3) ELISA Kit
EL0346c猪葡萄糖转运蛋白2(GLUT2)酶联免疫吸附测定试剂盒Porcine GLUT2 (Glucose Transporter 2) ELISA Kit
EL1922c猪葡萄糖转运蛋白1(GLUT1)酶联免疫吸附测定试剂盒 Porcine GLUT1 (Glucose Transporter 1) ELISA Kit
EL0043c猪谷氧还蛋白3(GLRX3)酶联免疫吸附测定试剂盒Porcine GLRX3 (Glutaredoxin 3) ELISA Kit
EL1790c猪胰高血糖素样肽2(GLP-2)酶联免疫吸附测定试剂盒 Porcine GLP-2 (Glucagon Like Peptide 2) ELISA Kit
EL0992c猪胰高血糖素样肽1(GLP-1)酶联免疫吸附测定试剂盒 Porcine GLP-1(Glucagon Like Peptide 1)ELISA Kit
EL0230c猪葡萄糖激酶调节蛋白(GKRP)酶联免疫吸附测定试剂盒 Porcine GKRP (Glucokinase Regulatory Protein) ELISA Kit
EL0515c猪胃动蛋白2(GKN2)酶联免疫吸附测定试剂盒Porcine GKN2 (Gastrokine 2) ELISA Kit
EL0634c猪胃动蛋白1(GKN1)酶联免疫吸附测定试剂盒Porcine GKN1 (Gastrokine 1) ELISA Kit
EL0263c猪间隙连接蛋白β1(GJβ1)酶联免疫吸附测定试剂盒Porcine GJβ1 (Gap Junction Protein Beta 1) ELISA Kit
EL0176c猪胃泌素抑制肽(GIP)酶联免疫吸附测定试剂盒 Porcine GIP(Gastric Inhibitory Polypeptide)ELISA Kit
EL0701c猪胃内因子(GIF)酶联免疫吸附测定试剂盒Porcine GIF (Gastric Intrinsic Factor) ELISA Kit
EL0642c猪促生长激素释放激素(GHRH)酶联免疫吸附测定试剂盒 Porcine GHRH (Growth Hormone Releasing Hormone) ELISA Kit
EL2048c猪生长激素受体(GHR)酶联免疫吸附测定试剂盒Porcine GHR (Growth Hormone Receptor) ELISA Kit
EL0719c猪生长激素诱导跨膜蛋白(GHITM)酶联免疫吸附测定试剂盒Porcine GHITM (Growth Hormone Inducible Transmembrane Protein) ELISA Kit
EL0577c猪抗生长激素抗体(GHAb)酶联免疫吸附测定试剂盒 Porcine GHAb (Anti-Growth Hormone Antibody) ELISA Kit
EL0527c猪生长激素2(GH2)酶联免疫吸附测定试剂盒Porcine GH2 (Growth Hormone 2) ELISA Kit
EL0075c猪促生长激素(GH)酶联免疫吸附测定试剂盒Porcine GH (Growth Hormone) ELISA Kit
EL0058c猪神经胶质纤维酸性蛋白(GFAP)酶联免疫吸附测定试剂盒Porcine GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
EL1167c猪鸟苷酸交换因子(GEF)酶联免疫吸附测定试剂盒 Porcine GEF (Guanylate Exchange Factor) ELISA Kit
EL1321c猪胶质细胞系来源的神经营养因子(GDNF)酶联免疫吸附测定试剂盒 Porcine GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
EL1869c猪GDP解离抑制因子1(GDI1)酶联免疫吸附测定试剂盒Porcine GDI1 (GDP Dissociation Inhibitor 1) ELISA Kit
EL1877c猪谷氨酸脱氢酶(GDH/GLDH)酶联免疫吸附测定试剂盒 Porcine GDH/GLDH (Glutamate dehydrogenase) ELISA Kit
EL0034c猪生长分化因子9(GDF9)酶联免疫吸附测定试剂盒Porcine GDF9 (Growth Differentiation Factor 9) ELISA Kit
EL2165c猪生长分化因子7(GDF7)酶联免疫吸附测定试剂盒Porcine GDF7 (Growth Differentiation Factor 7) ELISA Kit
EL0761c猪生长分化因子5(GDF5)酶联免疫吸附测定试剂盒Porcine GDF5 (Growth Differentiation Factor 5) ELISA Kit
EL0305c猪生长分化因子3(GDF3)酶联免疫吸附测定试剂盒Porcine GDF3 (Growth Differentiation Factor 3) ELISA Kit
EL2101c猪生长分化因子2(GDF2)酶联免疫吸附测定试剂盒Porcine GDF2 (Growth Differentiation Factor 2) ELISA Kit
EL0969c猪生长分化因子15(GDF15)酶联免疫吸附测定试剂盒Porcine GDF15 (Growth Differentiation Factor 15) ELISA Kit
EL0979c猪生长分化因子11(GDF11)酶联免疫吸附测定试剂盒Porcine GDF11 (Growth Differentiation Factor 11) ELISA Kit
EL1926c猪生长分化因子10(GDF10)酶联免疫吸附测定试剂盒Porcine GDF10 (Growth Differentiation Factor 10) ELISA Kit
EL1483c猪生长分化因子1(GDF1)酶联免疫吸附测定试剂盒Porcine GDF1 (Growth Differentiation Factor 1) ELISA Kit
EL1015c猪粒细胞集落刺激因子受体(GCSFR)酶联免疫吸附测定试剂盒Porcine GCSFR (Colony Stimulating Factor Receptor, Granulocyte) ELISA Kit
EL0837c猪粒细胞趋化蛋白2(GCP-2)酶联免疫吸附测定试剂盒Porcine GCP-2 (Granulocyte Chemotactic Protein 2) ELISA Kit
EL1867c猪谷氨酸半胱氨酸连接酶修饰亚基(GCLM)酶联免疫吸附测定试剂盒Porcine GCLM (Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit
EL0637c猪葡萄糖激酶(GCK)酶联免疫吸附测定试剂盒 Porcine GCK (Glucokinase) ELISA Kit
EL1533c猪糖盏蛋白(GC)酶联免疫吸附测定试剂盒Porcine GC (Glycocalicin) ELISA Kit
EL0535c猪胰高血糖素(GC)酶联免疫吸附测定试剂盒 Porcine GC (Glucagon) ELISA Kit
EL2080c猪鸟苷酸结合蛋白4(GBP4)酶联免疫吸附测定试剂盒 Porcine GBP4 (Guanylate Binding Protein 4) ELISA Kit
EL0145c猪GATA结合蛋白5(GATA5)酶联免疫吸附测定试剂盒Porcine GATA5 (GATA Binding Protein 5) ELISA Kit
EL0772c猪GATA结合蛋白4(GATA4)酶联免疫吸附测定试剂盒Porcine GATA4 (GATA Binding Protein 4) ELISA Kit
EL0575c猪GATA结合蛋白3(GATA3)酶联免疫吸附测定试剂盒Porcine GATA3 (GATA Binding Protein 3) ELISA Kit
EL1171c猪GATA结合蛋白2(GATA2)酶联免疫吸附测定试剂盒Porcine GATA2 (GATA Binding Protein 2) ELISA Kit
EL0578c猪GATA结合蛋白1(GATA1)酶联免疫吸附测定试剂盒Porcine GATA1 (GATA Binding Protein 1) ELISA Kit
EL1445c猪促胃液素受体(GasR)酶联免疫吸附测定试剂盒 Porcine GasR (Gastrin Receptor) ELISA Kit
EL0132c猪生长停滞特异性蛋白6(Gas6)酶联免疫吸附测定试剂盒Porcine Gas6 (Growth Arrest Specific Protein 6) ELISA Kit
EL0315c猪N-乙酰半乳糖胺-6-硫酸酯酶(GAS)酶联免疫吸附测定试剂盒Porcine GAS (N-Acetylgalactosamine 6-Sulfatase) ELISA Kit
EL1720c猪甘氨酰tRNA合成酶(GARS)酶联免疫吸附测定试剂盒 Porcine GARS (Glycyl tRNA Synthetase) ELISA Kit
EL2176c猪生长相关蛋白43(GAP43)酶联免疫吸附测定试剂盒 Porcine GAP43 (Growth Associated Protein 43) ELISA Kit
EL0507c富含亮氨酸重复序列Nogo受体反应蛋白4抗体猪GTP酶活化蛋白(GAP)酶联免疫吸附测定试剂盒 Porcine GAP (GTPase Activating Protein) ELISA Kit
EL2061c猪甘丙肽/甘丙素受体1(GALR1)酶联免疫吸附测定试剂盒 Porcine GALR1 (Galanin Receptor 1) ELISA Kit
EL0625c猪半乳凝集素9(GAL9)酶联免疫吸附测定试剂盒Porcine GAL9 (Galectin 9 ) ELISA Kit
EL0602c猪半乳凝集素8(GAL8)酶联免疫吸附测定试剂盒Porcine GAL8 (Galectin 8) ELISA Kit