瘦素抗体
『别名』LEP; Leptin Murine Obesity Homolog; Leptin Precursor Obesity Factor; OB; Obese Protein; Obesity; Obesity factor; Obesity homolog mouse; Obesity Murine Homolog Leptin; OBS.
『浓度』瘦素抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
瘦素抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
瘦素抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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ELB1970c兔白细胞抗原A(HLA-A)酶联免疫吸附测定试剂盒 Rabbit HLA-A (Leukocyte Antigen A) ELISA Kit
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ELB1271c兔低氧诱导因子1α (HIF-1α)酶联免疫吸附测定试剂盒Rabbit HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit
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ELB1468c兔肝细胞生长因子(HGF)酶联免疫吸附测定试剂盒 Rabbit HGF (Hepatocyte Growth Factor) ELISA Kit
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ELB0936c兔颗粒酶K(GZMK)酶联免疫吸附测定试剂盒 Rabbit GZMK (Granzyme K) ELISA Kit
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ELB0291c兔鸟苷酸激酶1(GUK1)酶联免疫吸附测定试剂盒 Rabbit GUK1 (Guanylate Kinase 1) ELISA Kit
ELB0800c兔鸟苷酸环化酶激活因子2B(GUCA2B)酶联免疫吸附测定试剂盒 Rabbit GUCA2B (Guanylate Cyclase Activator 2B) ELISA Kit
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ELB0711c兔通用转录因子II B(GTF2B)酶联免疫吸附测定试剂盒Rabbit GTF2B (General Transcription Factor IIB) ELISA Kit
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ELB0075c兔凝溶胶蛋白(GS)酶联免疫吸附测定试剂盒 Rabbit GS (Gelsolin) ELISA Kit
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