乳铁蛋白抗体
『别名』CKRX; GIG12; Growth inhibiting protein 12; HLF2; Homo sapiens chemokine receptor X; Kaliocin 1; Lactoferrin; Lactoferroxin A; Lactoferroxin B; Lactoferroxin C; Lactoferroxin-C; Lactotransferrin; LF; LTF; Neutrophil lactoferrin; Talalactoferrin alfa; Talalactoferrin; TRFL_HUMAN.
『浓度』乳铁蛋白抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
乳铁蛋白抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
乳铁蛋白抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
ELB0443c 兔淋巴细胞功能相关抗原1(LFA-1)酶联免疫吸附测定试剂盒 Rabbit LFA-1 (Lymphocyte Function Associated Antigen 1) ELISA Kit
ELB2257c 兔瘦素受体(LEPR)酶联免疫吸附测定试剂盒 Rabbit LEPR (Leptin Receptor) ELISA Kit
ELB1002c 兔瘦素(LEP)酶联免疫吸附测定试剂盒 Rabbit LEP (Leptin) ELISA Kit
ELB2226c 兔低密度脂蛋白受体(LDLR)酶联免疫吸附测定试剂盒 Rabbit LDLR (Low Density Lipoprotein Receptor) ELISA Kit
ELB0556c 兔低密度脂蛋白(LDL)酶联免疫吸附测定试剂盒 Rabbit LDL (Low Density Lipoprotein) ELISA Kit
ELB1053c 兔乳酸脱氢酶C(LDHC)酶联免疫吸附测定试剂盒 Rabbit LDHC (Lactate Dehydrogenase C) ELISA Kit
ELB1514c 兔乳酸脱氢酶B(LDHB)酶联免疫吸附测定试剂盒 Rabbit LDHB (Lactate Dehydrogenase B) ELISA Kit
ELB1500c 兔乳酸脱氢酶A(LDHA)酶联免疫吸附测定试剂盒 Rabbit LDHA (Lactate Dehydrogenase A) ELISA Kit
ELB2043c 兔脂质运载蛋白1(LCN1)酶联免疫吸附测定试剂盒 Rabbit LCN1 (Lipocalin 1) ELISA Kit
ELB1418c 兔白细胞共同抗原(LCA/CD45)酶联免疫吸附测定试剂盒 Rabbit LCA/CD45( Leukocyte Common Antigen) ELISA Kit
ELB1211c 兔脂多糖结合蛋白(LBP)酶联免疫吸附测定试剂盒 Rabbit LBP (Lipopolysaccharide Binding Protein) ELISA Kit
ELB0327c 兔长效甲状腺刺激素(LATS)酶联免疫吸附测定试剂盒 Rabbit LATS (Long Acting Thyroid Stimulator) ELISA Kit
ELB1884c 兔T细胞活化连接蛋白(LAT)酶联免疫吸附测定试剂盒 Rabbit LAT (Linker For Activation of T-cell) ELISA Kit
ELB1845c 兔亮氨酰氨基肽酶(LAP)酶联免疫吸附测定试剂盒 Rabbit LAP (Leucine Aminopeptidase) ELISA Kit
ELB2058c 兔B淋巴细胞激活抗原B7-2(LAB7-2/CD86)酶联免疫吸附测定试剂盒 Rabbit LAB7-2/CD86 (B-Lymphocyte Activation Antigen B7-2) ELISA Kit
ELB0828c 兔L1细胞粘附分子(L1CAM/CD171)酶联免疫吸附测定试剂盒 Rabbit L1CAM/CD171 (L1-Cell Adhesion Molecule) ELISA Kit
ELB1818c 兔激肽原1(KNG1)酶联免疫吸附测定试剂盒 Rabbit KNG1 (Kininogen 1) ELISA Kit
ELB1977c 兔激肽释放酶8(KLK8)酶联免疫吸附测定试剂盒 Rabbit KLK8 (Kallikrein 8) ELISA Kit
ELB0661c 兔激肽释放酶7(KLK7)酶联免疫吸附测定试剂盒 Rabbit KLK7 (Kallikrein 7) ELISA Kit
ELB1632c 兔激肽释放酶6(KLK6)酶联免疫吸附测定试剂盒 Rabbit KLK6 (Kallikrein 6) ELISA Kit
ELB1569c 兔肾损伤分子1(KIM-1)酶联免疫吸附测定试剂盒 Rabbit KIM-1 (Kidney Injury Molecule 1) ELISA Kit
ELB1516c 兔角膜蛋白(KERA)酶联免疫吸附测定试剂盒 Rabbit KERA (Keratocan) ELISA Kit
ELB0207c 兔角化细胞内分泌因子(KAF)酶联免疫吸附测定试剂盒 Rabbit KAF (Keratinocyte Autocrine Factor) ELISA Kit
ELB0510c 兔核因子κB抑制蛋白β(IκBβ)酶联免疫吸附测定试剂盒 Rabbit IκBβ (Inhibitory Subunit of NF Kappa B Beta) ELISA Kit
ELB0777c 兔核因子κB抑制蛋白α(IκBα)酶联免疫吸附测定试剂盒 Rabbit IκBα (Inhibitory Subunit of NF Kappa B Alpha) ELISA Kit
ELB1554c 兔内凝集蛋白1(ITLN1)酶联免疫吸附测定试剂盒 Rabbit ITLN1 (Intelectin 1/Omentin) ELISA Kit
ELB0274c 兔中间α球蛋白抑制因子H4(ITIH4)酶联免疫吸附测定试剂盒 Rabbit ITIH4 (Inter Alpha-Globulin Inhibitor H4) ELISA Kit
ELB1674c 兔干扰素诱导T细胞α亚族趋化因子 (I-TAC)酶联免疫吸附测定试剂盒 Rabbit I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit
ELB1477c 兔白介素1受体关联激酶3 (IRAK3)酶联免疫吸附测定试剂盒 Rabbit IRAK3 (Interleukin 1 Receptor Associated Kinase 3) ELISA Kit
ELB2187c 兔钙非依赖性磷脂酶A2(iPLA2)酶联免疫吸附测定试剂盒 Rabbit iPLA2 (Phospholipidase A2, Calcium Independent) ELISA Kit
ELB0578c 兔10kDa干扰素γ诱导蛋白(IP-10/CXCL10)酶联免疫吸附测定试剂盒 Rabbit IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
ELB1746c 兔胰岛素受体(INSR)酶联免疫吸附测定试剂盒 Rabbit INSR (Insulin Receptor) ELISA Kit
ELB0842c 兔胰岛素样蛋白5(INSL5)酶联免疫吸附测定试剂盒 Rabbit INSL5 (Insulin Like Protein 5) ELISA Kit
ELB2184c 兔胰岛素样蛋白3(INSL3)酶联免疫吸附测定试剂盒 Rabbit INSL3 (Insulin Like Protein 3) ELISA Kit
ELB0193c 兔白介素9(IL-9)酶联免疫吸附测定试剂盒 Rabbit IL-9 (Interleukin 9) ELISA Kit
ELB1142c 兔白介素8(IL-8)酶联免疫吸附测定试剂盒 Rabbit IL-8 (Interleukin 8) ELISA Kit
ELB2064c 兔白介素6受体(IL-6R)酶联免疫吸附测定试剂盒 Rabbit IL-6R (Interleukin 6 Receptor) ELISA Kit
ELB0014c 兔白介素6(IL-6)酶联免疫吸附测定试剂盒 Rabbit IL-6 (Interleukin 6) ELISA Kit
ELB1411c 兔白介素5(IL-5)酶联免疫吸附测定试剂盒 Rabbit IL-5 (Interleukin 5) ELISA Kit
ELB1486c 兔白介素4(IL-4)酶联免疫吸附测定试剂盒 Rabbit IL-4 (Interleukin 4) ELISA Kit
ELB1700c 兔白介素3(IL-3)酶联免疫吸附测定试剂盒 Rabbit IL-3 (Interleukin 3) ELISA Kit
ELB1161c 兔白介素2受体β(IL-2Rβ)酶联免疫吸附测定试剂盒 Rabbit IL-2Rβ (Interleukin 2 Receptor Beta) ELISA Kit
ELB0687c 兔白介素2受体α(IL-2Rα/CD25)酶联免疫吸附测定试剂盒 Rabbit IL-2Rα/CD25 (Interleukin 2 Receptor Alpha) ELISA Kit
ELB1958c 兔白介素24 (IL-24)酶联免疫吸附测定试剂盒 Rabbit IL-24 (Interleukin 24) ELISA Kit
ELB1174c 兔白介素23 p40 (IL-23 p40)酶联免疫吸附测定试剂盒 Rabbit IL-23 p40(Interleukin 23) ELISA Kit
ELB0722c 兔白介素23(IL-23)酶联免疫吸附测定试剂盒 Rabbit IL-23 (Interleukin 23) ELISA Kit
ELB1687c 兔白介素22(IL-22)酶联免疫吸附测定试剂盒 Rabbit IL-22 (Interleukin 22) ELISA Kit
ELB2097c 兔白介素20(IL-20)酶联免疫吸附测定试剂盒 Rabbit IL-20 (Interleukin 20) ELISA Kit
ELB1217c 兔白介素2(IL-2)酶联免疫吸附测定试剂盒 Rabbit IL-2 (Interleukin 2) ELISA Kit
ELB0013c 兔白介素1β(IL-1β)酶联免疫吸附测定试剂盒 Rabbit IL-1β (Interleukin 1 Beta) ELISA Kit
ELB1496c 兔白介素1α(IL-1α)酶联免疫吸附测定试剂盒 Rabbit IL-1α (Interleukin 1 Alpha) ELISA Kit
ELB0815c 兔白介素1受体拮抗剂(IL-1ra)酶联免疫吸附测定试剂盒 Rabbit IL-1ra (Interleukin 1 Receptor Antagonist) ELISA Kit
ELB2061c 兔白介素1受体II(IL-1R2)酶联免疫吸附测定试剂盒 Rabbit IL-1R2 (Interleukin 1 Receptor Type II) ELISA Kit
ELB0059c 兔白介素1受体Ⅰ(IL1R1)酶联免疫吸附测定试剂盒 Rabbit IL1R1 (Interleukin 1 Receptor Type I) ELISA Kit
ELB0337c 兔白介素19(IL-19)酶联免疫吸附测定试剂盒 Rabbit IL-19 (Interleukin 19) ELISA Kit
ELB1974c 兔白介素18乳铁蛋白抗体(IL-18)酶联免疫吸附测定试剂盒 Rabbit IL-18 (Interleukin 18) ELISA Kit
ELB1795c 兔白介素17(IL-17)酶联免疫吸附测定试剂盒 Rabbit IL-17 (Interleukin 17) ELISA Kit
ELB1539c 兔白介素16(IL-16)酶联免疫吸附测定试剂盒 Rabbit IL-16 (Interleukin 16) ELISA Kit
ELB1173c 兔白介素15(IL-15)酶联免疫吸附测定试剂盒 Rabbit IL-15 (Interleukin 15) ELISA Kit