?磷酸化白细胞F肌动蛋白结合蛋白抗体
『别名』47 kDa actin binding protein; 47 kDa actin-binding protein; 52 kDa phosphoprotein; F actin binding and cytoskeleton associated protein; Leufactin (leukocyte F-actin binding protein); Leufactin; Leukocyte F actin binding protein; Leukocyte specific protein 1; LSP 1; Lsp1; LSP1_HUMAN; Lymphocyte specific antigen WP34; Lymphocyte specific protein 1; Lymphocyte specific protein pp52; Lymphocyte-specific antigen WP34; Lymphocyte-specific protein 1; OTTHUMP00000014138; OTTHUMP00000014139; OTTHUMP00000014140; OTTHUMP00000069612; OTTHUMP00000069614; OTTHUMP00000069615; OTTHUMP00000069616; pp52; Protein pp52; S37 protein; WP 34; WP34. 『浓度』磷酸化白细胞F肌动蛋白结合蛋白抗体1mg/1ml
『规格』0.1ml/100μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
?磷酸化白细胞F肌动蛋白结合蛋白抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
磷酸化白细胞F肌动蛋白结合蛋白抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
CL0008c 大鼠粒细胞巨噬细胞集落刺激因子(GM-CSF)化学发光免疫分析试剂盒 Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit
EL0394c 大鼠β-半乳糖苷酶(GLβ)酶联免疫吸附测定试剂盒 Rat GLβ (Galactosidase, Beta) ELISA Kit
CL0258c 大鼠β-半乳糖苷酶(GLβ)化学发光免疫分析试剂盒 Rat GLβ (Galactosidase, Beta) CLIA Kit
EL0430c 大鼠葡萄糖转运蛋白4(Glut4)酶联免疫吸附测定试剂盒 Rat GLUT4 (Glucose Transporter 4)ELISA Kit
CL0287c 大鼠葡萄糖转运蛋白4(Glut4)化学发光免疫分析试剂盒 Rat GLUT4 (Glucose Transporter 4)CLIA Kit
EL0429c 大鼠葡萄糖转运蛋白3(Glut3)酶联免疫吸附测定试剂盒 Rat GLUT3 (Glucose Transporter 3) ELISA Kit
CL0286c 大鼠葡萄糖转运蛋白3(Glut3)化学发光免疫分析试剂盒 Rat GLUT3 (Glucose Transporter 3) CLIA Kit
EL0354c 大鼠葡萄糖转运蛋白2(Glut2)酶联免疫吸附测定试剂盒 Rat GLUT2 (Glucose Transporter 2)ELISA Kit
EL0353c 大鼠葡萄糖转运蛋白1(Glut1)酶联免疫吸附测定试剂盒 Rat GLUT1 (Glucose Transporter 1) ELISA Kit
EL0059c 大鼠胰高血糖素样肽1(GLP-1) 酶联免疫吸附测定试剂盒 Rat GLP-1 (Glucagon Like Peptide 1) ELISA Kit
CL0048c 大鼠胰高血糖素样肽1(GLP-1) 化学发光免疫分析试剂盒 Rat GLP-1 (Glucagon Like Peptide 1) CLIA Kit
EL0410c 大鼠胃动蛋白2(GKN2)酶联免疫吸附测定试剂盒 Rat GKN2 (Gastrokine 2) ELISA Kit
CL0272c 大鼠胃动蛋白2(GKN2)化学发光免疫分析试剂盒 Rat GKN2 (Gastrokine 2) CLIA Kit
EL0406c 大鼠缝隙连接蛋白β1(GJb1)酶联免疫吸附测定试剂盒 Rat GJb1(Gap Junction Protein Beta 1) ELISA Kit
EL0421c 大鼠糖皮质激素诱导的肿瘤坏死因子受体(GITR)酶联免疫吸附测定试剂盒 Rat GITR (Glucocorticoid Induced Tumor Necrosis Factor Receptor) ELISA Kit
CL0288c 大鼠葡萄糖依赖性胰岛素释放多肽(GIP)化学发光免疫分析试剂盒 Rat GIP (Glucose-Dependent Insulin-Releasing Polypeptide) CLIA Kit
EL0407c 大鼠胃泌素抑制肽(GIP)酶联免疫吸附测定试剂盒 Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit
CL0269c 大鼠胃泌素抑制肽(GIP)化学发光免疫分析试剂盒 Rat GIP (Gastric Inhibitory Polypeptide) CLIA Kit
EL0842c 大鼠饥饿素(GHRL)酶联免疫吸附测定试剂盒 Rat GHRL (Ghrelin) ELISA Kit
EL0469c 大鼠促生长激素释放激素(GHRH)酶联免疫吸附测定试剂盒 Rat GHRH (Growth Hormone Releasing Hormone) ELISA Kit
CL0318c 大鼠促生长激素释放激素(GHRH)化学发光免疫分析试剂盒 Rat GHRH (Growth Hormone Releasing Hormone) CLIA Kit
EL2553c 大鼠抗生长激素抗体(GHAb)酶联免疫吸附测定试剂盒 Rat GHAb (Anti-Growth Hormone Antibody) ELISA Kit
EL0468c 大鼠生长激素2(GH 2)酶联免疫吸附测定试剂盒 Rat GH2 (Growth Hormone 2) ELISA Kit
EL0029c 大鼠生长激素(GH)酶联免疫吸附测定试剂盒 Rat GH (Growth Hormone) ELISA Kit
CL0025c 大鼠生长激素(GH)化学发光免疫分析试剂盒 Rat GH (Growth Hormone) CLIA Kit
EL1428c 大鼠神经胶质纤维酸性蛋白(GFAP)酶联免疫吸附测定试剂盒 Rat GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
CL0740c 大鼠神经胶质纤维酸性蛋白(GFAP)化学发光免疫分析试剂盒 Rat GFAP (Glial Fibrillary Acidic Protein) CLIA Kit
EL0420c 大鼠胶质细胞系来源的神经营养因子(GDNF)酶联免疫吸附测定试剂盒 Rat GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
CL0278c 大鼠胶质细胞系来源的神经营养因子(GDNF)化学发光免疫分析试剂盒 Rat GDNF (Glial Cell Line Derived Neurotrophic Factor) CLIA Kit
EL2534c 大鼠胶质细胞源性连接蛋白(GDN)酶联免疫吸附测定试剂盒 Rat GDN(Glia Derived Nexin)ELISA Kit
EL0435c 大鼠谷氨酸脱氢酶(GDH)酶联免疫吸附测定试剂盒 Rat GDH (Glutamate Dehydrogenase) ELISA Kit
CL0292c 大鼠谷氨酸脱氢酶(GDH)化学发光免疫分析试剂盒 Rat GDH (Glutamate Dehydrogenase) CLIA Kit
EL0462c 大鼠生长分化因子9(GDF9)酶联免疫吸附测定试剂盒 Rat GDF9 (Growth Differentiation Factor 9) ELISA Kit
EL0461c 大鼠生长分化因子5(GDF5)酶联免疫吸附测定试剂盒 Rat GDF5 (Growth Differentiation Factor 5) ELISA Kit
CL0313c 大鼠生长分化因子5(GDF5)化学发光免疫分析试剂盒 Rat GDF5 (Growth Differentiation Factor 5) CLIA Kit
EL0460c 大鼠生长分化因子3(GDF3)酶联免疫吸附测定试剂盒 Rat GDF3 (Growth Differentiation Factor 3) ELISA Kit
CL0312c 大鼠生长分化因子3(GDF3)化学发光免疫分析试剂盒 Rat GDF3 (Growth Differentiation Factor 3) CLIA Kit
EL0459c 大鼠生长分化因子2(GDF2)酶联免疫吸附测定试剂盒 Rat GDF2 (Growth Differentiation Factor 2) ELISA Kit
CL0311c 大鼠生长分化因子2(GDF2)化学发光免疫分析试剂盒 Rat GDF2 (Growth Differentiation Factor 2) CLIA Kit
EL0464c 大鼠生长分化因子15(GDF15)酶联免疫吸附测定试剂盒 Rat GDF15 (Growth Differentiation Factor 15) ELISA Kit
CL0315c 大鼠生长分化因子15(GDF15)化学发光免疫分析试剂盒 Rat GDF15 (Growth Differentiation Factor 15) CLIA Kit
EL0463c 大鼠生长分化因子11(GDF11)酶联免疫吸附测定试剂盒 Rat GDF11(Growth Differentiation Factor 11)ELISA Kit
CL0314c 大鼠生长分化因子11(GDF11)化学发光免疫分析试剂盒 Rat GDF11 (Growth Differentiation Factor 11) CLIA Kit
EL0458c 大鼠生长分化因子1(GDF1)酶联免疫吸附测定试剂盒 Rat GDF1 (Growth Differentiation Factor 1) ELISA Kit
CL0310c 大鼠生长分化因子1(GDF1)化学发光免疫分析试剂盒 Rat GDF1 (Growth Differentiation Factor 1) CLIA Kit
EL0245c 大鼠粒细胞集落刺激因子受体(GCSFR)酶联免疫吸附测定试剂盒 Rat GCSFR (Colony Stimulating Factor Receptor, Granulocyte) ELISA Kit
CL0171c 大鼠粒细胞集落刺激因子受体(GCSFR)化学发光免疫分析试剂盒 Rat GCSFR (Colony Stimulating Factor Receptor, Granulocyte) CLIA Kit
EL0454c 大鼠粒细胞集落刺激因子(G-CSF)酶联免疫吸附测定试剂盒 Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit
CL0307c 大鼠粒细胞集落刺激因子(G-CSF)化学发光免疫分析试剂盒 Rat G-CSF (Granulocyte Colony Stimulating Factor) CLIA Kit
EL0453c 大鼠粒细胞趋化蛋白2(GCP-2)酶联免疫吸附测定试剂盒 Rat GCP-2 (Granulocyte Chemotactic Protein 2) ELISA Kit
CL0306c 大鼠粒细胞趋化蛋白2(GCP-2)化学发光免疫分析试剂盒 Rat GCP-2 (Granulocyte Chemotactic磷酸化白细胞F肌动蛋白结合蛋白抗体 Protein 2) CLIA Kit EL0433c 大鼠谷氨酸半胱氨酸连接酶修饰亚基(GCLM)酶联免疫吸附测定试剂盒 Rat GCLM (Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit
CL0290c 大鼠谷氨酸半胱氨酸连接酶修饰亚基(GCLM)化学发光免疫分析试剂盒 Rat GCLM (Glutamate Cysteine Ligase, Modifier Subunit) CLIA Kit
EL0426c 大鼠葡萄糖激酶(GCK)酶联免疫吸附测定试剂盒 Rat GCK (Glucokinase) ELISA Kit
CL0283c 大鼠葡萄糖激酶(GCK)化学发光免疫分析试剂盒 Rat GCK (Glucokinase) CLIA Kit