20号染色体开放阅读框19抗体
『别名』DKFZP586H021; HT013; KIZUNA; MGC102941; MGC141930; Uncharacterized protein C20orf19; KIZ_HUMAN.
『浓度』20号染色体开放阅读框19抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
20号染色体开放阅读框19抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
20号染色体开放阅读框19抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
ELK0638c 猴Bcl2关联永生基因3(BAG3)酶联免疫吸附测定试剂盒 Monkey BAG3 (Bcl2 Associated Athanogene 3) ELISA Kit
ELK1904c 猴B细胞活化因子受体(BAFFR)酶联免疫吸附测定试剂盒 Monkey BAFFR (B Cell Activating Factor Receptor) ELISA Kit
ELK1489c 猴B细胞活化因子 (BAFF/CD257/TNFSF13B)酶联免疫吸附测定试剂盒 Monkey BAFF/CD257/TNFSF13B (B-Cell Activating Factor) ELISA Kit
ELK0053c 猴BCL2相关死亡促进因子因子(BAD)酶联免疫吸附测定试剂盒 Monkey BAD (Bcl2 Associated Death Promoter) ELISA Kit
ELK1563c 猴β位淀粉样前体蛋白裂解酶2(BACE2)酶联免疫吸附测定试剂盒 Monkey BACE2 (β-site APP Cleaving Enzyme 2) ELISA Kit
ELK0890c 猴β位淀粉样前体蛋白裂解酶1(BACE1)酶联免疫吸附测定试剂盒 Monkey BACE1 (Beta-site APP Cleaving Enzyme 1) ELISA Kit
ELK1824c 猴β淀粉样蛋白1-42(Aβ1-42)酶联免疫吸附测定试剂盒 Monkey Aβ1-42 (Amyloid Beta Peptide 1-42) ELISA Kit
ELK0477c 猴β淀粉样蛋白1-40(Aβ1-40)酶联免疫吸附测定试剂盒 Monkey Aβ1-40 (Amyloid Beta Peptide 1-40) ELISA Kit
ELK1130c 猴天青杀素1(AZU1)酶联免疫吸附测定试剂盒 Monkey AZU1 (Azurocidin 1) ELISA Kit
ELK0274c 猴轴蛋白抑制蛋白2(AXIN2)酶联免疫吸附测定试剂盒 Monkey AXIN2 (Axis Inhibition Protein 2) ELISA Kit
ELK1383c 猴精氨酸加压素受体1A(AVPR1A)酶联免疫吸附测定试剂盒 Monkey AVPR1A (Arginine Vasopressin Receptor 1A) ELISA Kit
ELK0493c 猴失调症蛋白质2(ATXN2)酶联免疫吸附测定试剂盒 Monkey ATXN2 (Ataxin 2) ELISA Kit
ELK0914c 猴失调症蛋白质1(ATXN1)酶联免疫吸附测定试剂盒 Monkey ATXN1 (Ataxin 1) ELISA Kit
ELK2237c 猴毛细血管扩张性共济失调突变因子(ATM)酶联免疫吸附测定试剂盒 Monkey ATM (Ataxia Telangiectasia Mutated) ELISA Kit
ELK1234c 猴抗甲状腺球蛋白抗体(ATGA/TGAB)酶联免疫吸附测定试剂盒 Monkey ATGA/TGAB (Anti-Thyroid-Globulin Antibody) ELISA Kit
ELK0860c 猴转录激活因子7(ATF7)酶联免疫吸附测定试剂盒 Monkey ATF7 (Activating Transcription Factor 7) ELISA Kit
ELK0133c 猴转录激活因子6(ATF6)酶联免疫吸附测定试剂盒 Monkey ATF6 (Activating Transcription Factor 6) ELISA Kit
ELK0616c 猴转录激活因子-5(ATF-5)酶联免疫吸附测定试剂盒 Monkey ATF-5 (Activating Transcription Factor 5) ELISA Kit
ELK0453c 猴转录激活因子-4(ATF-4)酶联免疫吸附测定试剂盒 Monkey ATF-4 (Activating Transcription Factor 4) ELISA Kit
ELK0209c 猴转录激活因子-3(ATF-3)酶联免疫吸附测定试剂盒 Monkey ATF-3 (Activating Transcription Factor 3) ELISA Kit
ELK0411c 猴转录激活因子-2(ATF-2)酶联免疫吸附测定试剂盒 Monkey ATF-2 (Activating Transcription Factor 2) ELISA Kit
ELK0712c 猴转录激活因子-1(ATF-1)酶联免疫吸附测定试剂盒 Monkey ATF-1 (Activating Transcription Factor 1) ELISA Kit
ELK1131c 猴血管紧张肽酶A(ATA)酶联免疫吸附测定试剂盒 Monkey ATA (Angiotensinase A) ELISA Kit
ELK0090c 猴抗凝血酶(AT)酶联免疫吸附测定试剂盒 Monkey AT (AntiThrombin) ELISA Kit
ELK2205c 猴天门冬氨酸氨基转移酶(AST)酶联免疫吸附测定试剂盒 Monkey AST (Aspartate Aminotransferase) ELISA Kit
ELK0326c 猴无孢蛋白(ASPN)酶联免疫吸附测定试剂盒 Monkey ASPN (Asporin) ELISA Kit
ELK1849c 猴酸性神经鞘磷脂酶(ASM)酶联免疫吸附测定试剂盒 Monkey ASM (Acid Sphingomyelinase) ELISA Kit
ELK1598c 猴凋亡信号调节激酶I(ASK-1)酶联免疫吸附测定试剂盒 Monkey ASK-1 (Apoptosis Signal Regulating Kinase 1) ELISA Kit
ELK0419c 猴去唾液酸糖蛋白受体2(ASGR2)酶联免疫吸附测定试剂盒 Monkey ASGR2 (Asialoglycoprotein Receptor 2) ELISA Kit
ELK1983c 猴去唾液酸糖蛋白受体1(ASGR1)酶联免疫吸附测定试剂盒 Monkey ASGR1 (Asialoglycoprotein Receptor 1) ELISA Kit
ELK0893c 猴芳基硫酸酯酶B(ASB)酶联免疫吸附测定试剂盒 Monkey ASB (Arylsulfatase B) ELISA Kit
ELK1320c 猴芳基硫酸酯酶A(ARSA)酶联免疫吸附测定试剂盒 Monkey ARSA (Arylsulfatase A) ELISA Kit
ELK1968c 猴抑制蛋白β2(ARRβ2)酶联免疫吸附测定试剂盒 Monkey ARRβ2 (Arrestin Beta 2) ELISA Kit
ELK0420c 猴抑制蛋白β1(ARRβ1)酶联免疫吸附测定试剂盒 Monkey ARRβ1 (Arrestin Beta 1) ELISA Kit
ELK0027c 猴芳香酶(ARO)酶联免疫吸附测定试剂盒 Monkey ARO (Aromatase) ELISA Kit
ELK0826c 猴Rho GDP解离抑制因子α(ARHGDIα)酶联免疫吸附测定试剂盒 Monkey ARHGDIα (Rho GDP Dissociation Inhibitor Alpha ) ELISA Kit
ELK1969c 猴精氨酸酶(Arg)酶联免疫吸附测定试剂盒 Monkey Arg (Arginase) ELISA Kit
ELK1821c 猴雄激素受体(AR)酶联免疫吸附测定试剂盒 Monkey AR (Androgen Receptor) ELISA Kit
ELK1132c 猴双调蛋白(AR)酶联免疫吸附测定试剂盒 Monkey AR (Amphiregulin) ELISA Kit
ELK0361c 猴水通道蛋白4(AQP-4)酶联免疫吸附测定试剂盒 Monkey AQP-4 (Aquaporin 4) ELISA Kit
ELK0429c 猴水通道蛋白3(AQP-3)酶联免疫吸附测定试剂盒 Monkey AQP-3 (Aquaporin 3) ELISA Kit
ELK1899c 猴水通道蛋白2(AQP-2)酶联免疫吸附测定试剂盒 Monkey AQP-2 (Aquaporin 2) ELISA Kit
ELK1599c 猴水通道蛋白1(AQP-1)酶联免疫吸附测定试剂盒 Monkey AQP-1 (Aquaporin 1) ELISA Kit
ELK1034c 猴异常凝血酶原(APT)酶联免疫吸附测定试剂盒 Monkey APT (Abnormal Prothrombin) ELISA Kit
ELK0701c 猴抗磷脂酰丝氨酸抗体(APSA)酶联免疫吸附测定试剂盒 Monkey APSA (Anti-Phosphatidyl Serine Antibody) ELISA Kit
ELK2149c 猴淀粉样前体蛋白(APP)酶联免疫吸附测定试剂盒 Monkey APP (Amyloid Precursor Protein) ELISA Kit
ELK0983c 猴载脂蛋白M(ApoM)酶联免疫吸附测定试剂盒 Monkey ApoM (Apolipoprotein M) ELISA Kit
ELK1070c 猴载脂蛋白H(ApoH)酶联免疫吸附测定试剂盒 Monkey ApoH (Apolipoprotein H) ELISA Kit
ELK0037c 猴载脂蛋白F(ApoF)酶联免疫吸附测定试剂盒 Monkey ApoF (Apolipoprotein F) ELISA Kit
ELK1941c 猴载脂蛋白E(ApoE)酶联免疫吸附测定试剂盒 Monkey ApoE (Apolipoprotein E) ELISA Kit
ELK2217c 猴载脂蛋白D(ApoD)酶联免疫吸附测定试剂盒 Monkey ApoD (Apolipoprotein D) ELISA Kit
ELK1447c 猴载脂蛋白C4(ApoC4)酶联免疫吸附测定试剂盒 Monkey ApoC4 (Apolipoprotein C4) ELISA Kit
ELK0001c 猴载脂蛋白C3(ApoC3)酶联免疫吸附测定试剂盒 Monkey ApoC3(Apolipoprotein C3)ELISA Kit
ELK1665c 猴载脂蛋白C2(ApoC2)酶联免疫吸附测定试剂盒 Monkey ApoC2 (Apolipoprotein C2) ELISA Kit
ELK1767c 猴载脂蛋白C1(ApoC1)酶联免疫吸附测定试剂盒 Monkey ApoC1 (Apolipoprotein C1) ELISA Kit
ELK0044c 猴载脂蛋白B100(ApoB100)酶联免疫吸附测定试剂盒 Monkey ApoB100 (Apolipoprotein B100) ELISA Kit
ELK1742c 猴载脂蛋白B(ApoB)酶联免疫吸附测定试剂盒 Monkey ApoB (Apolipoprotein B) ELISA Kit
ELK0563c 猴载脂蛋白A5(ApoA5)酶联免疫吸附测定试剂盒 Monkey ApoA5 (Apolipoprotein A5) ELISA Kit
ELK1995c 猴载脂蛋白A420号染色体开放阅读框19抗体(ApoA4)酶联免疫吸附测定试剂盒 Monkey ApoA4 (Apolipoprotein A4) ELISA Kit
ELK1578c 猴载脂蛋白A2(ApoA2)酶联免疫吸附测定试剂盒 Monkey ApoA2 (Apolipoprotein A2) ELISA Kit
ELK1491c 猴载脂蛋白A1(ApoA1)酶联免疫吸附测定试剂盒 Monkey ApoA1 (Apolipoprotein A1) ELISA Kit
ELK1452c 猴抗载脂蛋白A1抗体(Apo A1-Ab)酶联免疫吸附测定试剂盒 Monkey Apo A1-Ab (Anti-Apolipoprotein A1 antibody) ELISA Kit