驱动蛋白KNS1抗体
『别名』Conventional kinesin heavy chain; KIF5B; Kinesin 1 (110-120kD); Kinesin family member 5B; Kinesin heavy chain; Kinesin heavy chain ubiquitous; Kinesin-1 heavy chain; KINH; KINH_HUMAN; KNS 1; KNS; KNS1; Ubiquitous kinesin heavy chain; UKHC.
『浓度』驱动蛋白KNS1抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
驱动蛋白KNS1抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
驱动蛋白KNS1抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
ELK0119c 猴血浆α颗粒膜蛋白(GMP-140)酶联免疫吸附测定试剂盒 Monkey GMP-140 (Alpha-granular Membrane Protein) ELISA Kit
ELK1440c 猴神经胶质成熟因子β(GMFβ)酶联免疫吸附测定试剂盒 Monkey GMFβ (Glia Maturation Factor, Beta) ELISA Kit
ELK1313c 猴粒细胞巨噬细胞集落刺激因子(GMCSF/CSF2)酶联免疫吸附测定试剂盒 Monkey GMCSF/CSF2(Granulocyte Macrophage Colony Stimulating Factor 2) ELISA Kit
ELK1734c 猴粒细胞巨噬细胞集落刺激因子抗体(GM-CSF Ab)酶联免疫吸附测定试剂盒 Monkey GM-CSF Ab (Anti-Granulocyte Macrophage Colony Stimulating Factor Antibody) ELISA Kit
ELK1894c 猴胃粘液素(GM)酶联免疫吸附测定试剂盒 Monkey GM (Gastric Mucin) ELISA Kit
ELK0840c 猴半乳糖苷酶β(GLβ)酶联免疫吸附测定试剂盒 Monkey GLβ (Galactosidase Beta) ELISA Kit
ELK0545c 猴半乳糖苷酶α(GLα)酶联免疫吸附测定试剂盒 Monkey GLα (Galactosidase Alpha) ELISA Kit
ELK0848c 猴糖基化依赖的细胞黏附分子(GlyCAM1)酶联免疫吸附测定试剂盒 Monkey GlyCAM1 (Glycosylation Dependent Cell Adhesion Molecule 1) ELISA Kit
ELK0993c 猴葡萄糖转运蛋白4(GLUT4)酶联免疫吸附测定试剂盒 Monkey GLUT4 (Glucose Transporter 4) ELISA Kit
ELK1073c 猴葡萄糖转运蛋白3(GLUT3)酶联免疫吸附测定试剂盒 Monkey GLUT3 (Glucose Transporter 3) ELISA Kit
ELK0386c 猴葡萄糖转运蛋白2(GLUT2)酶联免疫吸附测定试剂盒 Monkey GLUT2 (Glucose Transporter 2) ELISA Kit
ELK0529c 猴葡萄糖转运蛋白1(GLUT1)酶联免疫吸附测定试剂盒 Monkey GLUT1 (Glucose Transporter 1) ELISA Kit
ELK0100c 猴谷氧还蛋白3(GLRX3)酶联免疫吸附测定试剂盒 Monkey GLRX3 (Glutaredoxin 3) ELISA Kit
ELK2034c 猴胰高血糖素样肽2(GLP-2)酶联免疫吸附测定试剂盒 Monkey GLP-2 (Glucagon Like Peptide 2) ELISA Kit
ELK2265c 猴胰高血糖素样肽1活性形式(GLP-1,active form)酶联免疫吸附测定试剂盒 Monkey GLP-1,active form (Glucagon Like Peptide 1,active form) ELISA Kit
ELK0905c 猴胰高血糖素样肽1(GLP-1)酶联免疫吸附测定试剂盒 Monkey GLP-1 (Glucagon Like Peptide 1) ELISA Kit
ELK1499c 猴葡萄糖激酶调节蛋白(GKRP)酶联免疫吸附测定试剂盒 Monkey GKRP (Glucokinase Regulatory Protein) ELISA Kit
ELK2244c 猴胃动蛋白2(GKN2)酶联免疫吸附测定试剂盒 Monkey GKN2 (Gastrokine 2) ELISA Kit
ELK2140c 猴胃动蛋白1(GKN1)酶联免疫吸附测定试剂盒 Monkey GKN1 (Gastrokine 1) ELISA Kit
ELK2069c 猴间隙连接蛋白β1(GJβ1)酶联免疫吸附测定试剂盒 Monkey GJβ1 (Gap Junction Protein Beta 1) ELISA Kit
ELK0774c 猴胃泌素抑制肽(GIP)酶联免疫吸附测定试剂盒 Monkey GIP (Gastric Inhibitory Polypeptide) ELISA Kit
ELK1802c 猴胃内因子(GIF)酶联免疫吸附测定试剂盒 Monkey GIF (Gastric Intrinsic Factor) ELISA Kit
ELK1556c 猴促生长激素释放激素(GHRH)酶联免疫吸附测定试剂盒 Monkey GHRH (Growth Hormone Releasing Hormone) ELISA Kit
ELK1288c 猴生长激素受体(GHR)酶联免疫吸附测定试剂盒 Monkey GHR (Growth Hormone Receptor) ELISA Kit
ELK1208c 猴生长激素诱导跨膜蛋白(GHITM)酶联免疫吸附测定试剂盒 Monkey GHITM (Growth Hormone Inducible Transmembrane Protein) ELISA Kit
ELK0081c 猴抗生长激素抗体(GHAb)酶联免疫吸附测定试剂盒 Monkey GHAb (Anti-Growth Hormone Antibody) ELISA Kit
ELK1630c 猴生长激素2(GH2)酶联免疫吸附测定试剂盒 Monkey GH2 (Growth Hormone 2) ELISA Kit
ELK0056c 猴促生长激素(GH)酶联免疫吸附测定试剂盒 Monkey GH (Growth Hormone) ELISA Kit
ELK1769c 猴神经胶质纤维酸性蛋白(GFAP)酶联免疫吸附测定试剂盒 Monkey GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
ELK1520c 猴鸟苷酸交换因子(GEF)酶联免疫吸附测定试剂盒 Monkey GEF (Guanylate Exchange Factor) ELISA Kit
ELK0227c 猴胶质细胞系来源的神经营养因子(GDNF)酶联免疫吸附测定试剂盒 Monkey GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
ELK0465c 猴GDP解离抑制因子1(GDI1)酶联免疫吸附测定试剂盒 Monkey GDI1 (GDP Dissociation Inhibitor 1) ELISA Kit
ELK0791c 猴谷氨酸脱氢酶(GDH/GLDH)酶联免疫吸附测定试剂盒 Monkey GDH/GLDH (Glutamate dehydrogenase) ELISA Kit
ELK1972c 猴生长分化因子9(GDF9)酶联免疫吸附测定试剂盒 Monkey GDF9 (Growth Differentiation Factor 9) ELISA Kit
ELK2141c 猴生长分化因子7(GDF7)酶联免疫吸附测定试剂盒 Monkey GDF7 (Growth Differentiation Factor 7) ELISA Kit
ELK1882c 猴生长分化因子5(GDF5)酶联免疫吸附测定试剂盒 Monkey GDF5 (Growth Differentiation Factor 5) ELISA Kit
ELK1048c 猴生长分化因子3(GDF3)酶联免疫吸附测定试剂盒 Monkey GDF3 (Growth Differentiation Factor 3) ELISA Kit
ELK1378c 猴生长分化因子2(GDF2)酶联免疫吸附测定试剂盒 Monkey GDF2 (Growth Differentiation Factor 2) ELISA Kit
ELK0374c 猴生长分化因子15(GDF15)酶联免疫吸附测定试剂盒 Monkey GDF15 (Growth Differentiation Factor 15) ELISA Kit
ELK2111c 猴生长分化因子11(GDF11)酶联免疫吸附测定试剂盒 Monkey GDF11 (Growth Differentiation Factor 11) ELISA Kit
ELK0708c 猴生长分化因子10(GDF10)酶联免疫吸附测定试剂盒 Monkey GDF10 (Growth Differentiation Factor 10) ELISA Kit
ELK1429c 猴生长分化因子1(GDF1)酶联免疫吸附测定试剂盒 Monkey GDF1 (Growth Differentiation Factor 1) ELISA Kit
ELK1798c 猴粒细胞集落刺激因子受体(GCSFR)酶联免疫吸附测定试剂盒 Monkey GCSFR (Colony Stimulating Factor Receptor, Granulocyte) ELISA Kit
ELK0728c 猴粒细胞趋化蛋白2(GCP-2)酶联免疫吸附测定试剂盒 Monkey GCP-2 (Granulocyte Chemotactic Protein 2) ELISA Kit
ELK1041c 猴谷氨酸半胱氨酸连接酶修饰亚基(GCLM)酶联免疫吸附测定试剂盒 Monkey GCLM (Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit
ELK1886c 猴葡萄糖激酶(GCK)酶联免疫吸附测定试剂盒 Monkey GCK (Glucokinase) ELISA Kit
ELK0926c 猴糖盏蛋白(GC)酶联免疫吸附测定试剂盒 Monkey GC (Glycocalicin) ELISA Kit
ELK1192c 猴胰高血糖素(GC)酶联免疫吸附测定试剂盒 Monkey GC (Glucagon) ELISA Kit
ELK1548c 猴鸟苷酸结合蛋白4(GBP4)酶联免疫吸附测定试剂盒 Monkey GBP4 (Guanylate Binding Protein 4) ELISA Kit
ELK0945c 猴GATA结合蛋白5(GATA5)酶联免疫吸附测定试剂盒 Monkey GATA5 (GATA Binding Protein 5) ELISA Kit
ELK1498c 猴GATA结合蛋白4(GATA4)酶联免疫吸附测定试剂盒 Monkey GATA4 (GATA Binding Protein 4) ELISA Kit
ELK0647c 猴GATA结合蛋白3(GATA3)酶联免疫吸附测定试剂盒 Monkey GATA3 (GATA Binding Protein 3) ELISA Kit
ELK1335c 猴GATA结合蛋白2(GATA2)酶联免疫吸附测定试剂盒 Monkey GATA2 (GATA Binding Protein 2) ELISA Kit
ELK0437c 猴GATA结合蛋白1(GATA1)酶联免疫吸附测定试剂盒 Monkey GATA1 (GATA Binding Protein 1) ELISA Kit
ELK1639c 猴促胃液素受体驱动蛋白KNS1抗体(GasR)酶联免疫吸附测定试剂盒 Monkey GasR (Gastrin Receptor) ELISA Kit
ELK1647c 猴生长停滞特异性蛋白6(Gas6)酶联免疫吸附测定试剂盒 Monkey Gas6 (Growth Arrest Specific Protein 6) ELISA Kit
ELK1351c 猴N-乙酰半乳糖胺-6-硫酸酯酶(GAS)酶联免疫吸附测定试剂盒 Monkey GAS (N-Acetylgalactosamine 6-Sulfatase) ELISA Kit
ELK0373c 猴甘氨酰tRNA合成酶(GARS)酶联免疫吸附测定试剂盒 Monkey GARS (Glycyl tRNA Synthetase) ELISA Kit
ELK1501c 猴生长相关蛋白43(GAP43)酶联免疫吸附测定试剂盒 Monkey GAP43 (Growth Associated Protein 43) ELISA Kit