神经细胞钾氯离子转运蛋白抗体
『别名』Electroneutral potassium chloride cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Erythroid K Cl cotransporter 2; Erythroid K-Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2; KIAA1176; Neuronal K Cl cotransporter; Neuronal K-Cl cotransporter; S12A5; S12A5_HUMAN; SLC12A5; Solute carrier family 12 (potassium chloride transporter) member 5; Solute carrier family 12 member 5.
『浓度』神经细胞钾氯离子转运蛋白抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
神经细胞钾氯离子转运蛋白抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
神经细胞钾氯离子转运蛋白抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
ELK1795c 猴通用转录因子II B(GTF2B)酶联免疫吸附测定试剂盒 Monkey GTF2B (General Transcription Factor IIB) ELISA Kit
ELK1014c 猴通用转录因子II A肽1(GTF2A1)酶联免疫吸附测定试剂盒 Monkey GTF2A1 (General Transcription Factor IIA, Polypeptide 1) ELISA Kit
ELK1305c 猴肾小球组织糖基化终末产物(GTEAGE)酶联免疫吸附测定试剂盒 Monkey GTEAGE (Glomerular Tissue Extract Advanced Glycosylation End Products) ELISA Kit
ELK2124c 猴通用转录复合体(GTC)酶联免疫吸附测定试剂盒 Monkey GTC (General Transcription Complex) ELISA Kit
ELK1346c 猴胃泌素34(GT-34)酶联免疫吸附测定试剂盒 Monkey GT-34 (Gastrin 34) ELISA Kit
ELK1417c 猴谷胱甘肽S转移酶ω1(GSTω1)酶联免疫吸附测定试剂盒 Monkey GSTω1 (Glutathione S Transferase Omega 1) ELISA Kit
ELK0743c 猴谷胱甘肽S转移酶μ2(GSTμ2/GSTm2)酶联免疫吸附测定试剂盒 Monkey GSTμ2/GSTm2 (Glutathione S Transferase Mu 2, Muscle) ELISA Kit
ELK0109c 猴谷胱甘肽S转移酶κ1(GSTκ1)酶联免疫吸附测定试剂盒 Monkey GSTκ1 (Glutathione S Transferase Kappa 1) ELISA Kit
ELK2110c 猴谷胱甘肽S转移酶θ2(GSTθ2/GSTt2)酶联免疫吸附测定试剂盒 Monkey GSTθ2/GSTt2 (Glutathione S Transferase Theta 2) ELISA Kit
ELK0770c 猴谷胱甘肽S-转移酶θ1(GSTθ1/GSTt1)酶联免疫吸附测定试剂盒 Monkey GSTθ1/GSTt1 (Glutathione S Transferase Theta 1) ELISA Kit
ELK0589c 猴谷胱甘肽S转移酶α5(GSTα5)酶联免疫吸附测定试剂盒 Monkey GSTα5 (Glutathione S Transferase Alpha 5) ELISA Kit
ELK0706c 猴谷胱甘肽S转移酶α4(GSTα4)酶联免疫吸附测定试剂盒 Monkey GSTα4 (Glutathione S Transferase Alpha 4) ELISA Kit
ELK1727c 猴谷胱甘肽S-转移酶α1(GSTα1)酶联免疫吸附测定试剂盒 Monkey GSTα1 (Glutathione S Transferase Alpha 1) ELISA Kit
ELK1113c 猴谷胱甘肽S转移酶(GSTs)酶联免疫吸附测定试剂盒 Monkey GSTs (Glutathione S-Transferases) ELISA Kit
ELK1453c 猴谷胱甘肽S转移酶pi基因(GSTpi)酶联免疫吸附测定试剂盒 Monkey GSTpi (Glutathione S Transferases Pi) ELISA Kit
ELK0474c 猴糖化蛋白(GSP)酶联免疫吸附测定试剂盒 Monkey GSP (Glycosylated Serum Protein) ELISA Kit
ELK0211c 猴糖原合成酶激酶3β(GSK3β)酶联免疫吸附测定试剂盒 Monkey GSK3β (Glycogen Synthase Kinase 3 Beta) ELISA Kit
ELK0771c 猴糖原合成酶激酶3α(GSK3α)酶联免疫吸附测定试剂盒 Monkey GSK3α (Glycogen Synthase Kinase 3 Alpha) ELISA Kit
ELK0340c 猴粒细胞特异性抗核抗体(GSANA)酶联免疫吸附测定试剂盒 Monkey GSANA (Granulocyte Specific Anti-Nuclear Antibody) ELISA Kit
ELK0918c 猴凝溶胶蛋白(GS)酶联免疫吸附测定试剂盒 Monkey GS (Gelsolin) ELISA Kit
ELK0787c 猴糖皮质激素受体β(GRβ)酶联免疫吸附测定试剂盒 Monkey GRβ (Glucocorticoid Receptor Beta) ELISA Kit
ELK1097c 猴糖皮质激素受体α(GRα)酶联免疫吸附测定试剂盒 Monkey GRα (Glucocorticoid Receptor Alpha) ELISA Kit
ELK2113c 猴生长调节致癌基因γ(GROγ)酶联免疫吸附测定试剂盒 Monkey GROγ (Growth Regulated Oncogene Gamma) ELISA Kit
ELK2068c 猴生长调节致癌基因β(GROβ)酶联免疫吸附测定试剂盒 Monkey GROβ (Growth Regulated Oncogene Beta) ELISA Kit
ELK2105c 猴生长调节致癌基因α(GROα/CXCL1/NAP3)酶联免疫吸附测定试剂盒 Monkey GROα/CXCL1/NAP3 (Growth Regulated Oncogene Alpha) ELISA Kit
ELK1461c 猴颗粒体蛋白(GRN)酶联免疫吸附测定试剂盒 Monkey GRN (Granulin) ELISA Kit
ELK0057c 猴红藻氨酸离子型谷氨酸受体2(GRIK2)酶联免疫吸附测定试剂盒 Monkey GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2) ELISA Kit
ELK1682c 猴骨形态形成蛋白拮抗蛋白1(GREM1)酶联免疫吸附测定试剂盒 Monkey GREM1 (Gremlin 1) ELISA Kit
ELK1833c 猴生长因子受体结合蛋白7(Grb7)酶联免疫吸附测定试剂盒 Monkey Grb7 (Growth Factor Receptor Bound Protein 7) ELISA Kit
ELK0491c 猴生长因子受体结合蛋白2(Grb2)酶联免疫吸附测定试剂盒 Monkey Grb2 (Growth Factor Receptor Bound Protein 2) ELISA Kit
ELK1870c 猴生长因子受体结合蛋白14(Grb14)酶联免疫吸附测定试剂盒 Monkey Grb14 (Growth Factor Receptor Bound Protein 14) ELISA Kit
ELK1558c 猴生长因子受体结合蛋白10(Grb10)酶联免疫吸附测定试剂盒 Monkey Grb10 (Growth Factor Receptor Bound Protein 10) ELISA Kit
ELK0831c 猴谷胱甘肽还原酶(GR)酶联免疫吸附测定试剂盒 Monkey GR (Glutathione Reductase) ELISA Kit
ELK2236c 猴血浆谷胱甘肽过氧化物酶(GPX3)酶联免疫吸附测定试剂盒 Monkey GPX3 (Glutathione Peroxidase 3, Plasma) ELISA Kit
ELK0232c 猴谷胱甘肽过氧化物酶 1(GPX1)酶联免疫吸附测定试剂盒 Monkey GPX1 (Glutathione Peroxidase 1) ELISA Kit
ELK2135c 猴G蛋白偶联受体家族C5组成员(GPRC5A)酶联免疫吸附测定试剂盒 Monkey GPRC5A (G Protein Coupled Receptor, Family C, Group 5, Member A) ELISA Kit
ELK0912c 猴G蛋白偶联受体37(GPR37)酶联免疫吸附测定试剂盒 Monkey GPR37 (G Protein Coupled Receptor 37) ELISA Kit
ELK0630c 猴G蛋白偶联受体120(GPR120)酶联免疫吸附测定试剂盒 Monkey GPR120 (G Protein Coupled Receptor 120) ELISA Kit
ELK1749c 猴糖基化磷脂酰肌醇特异性磷脂酶D1(GPLD1)酶联免疫吸附测定试剂盒 Monkey GPLD1 (Glycosylphosphatidylinositol Specific Phospholipase D1) ELISA Kit
ELK0801c 猴血小板膜糖蛋白II b/III a(GPIIb/IIIa)酶联免疫吸附测定试剂盒 Monkey GPIIb/IIIa (Platelet Membrane Glycoprotein IIb/IIIa) ELISA Kit
ELK1024c 猴糖磷脂酰肌醇(GPI)酶联免疫吸附测定试剂盒 Monkey GPI (Glycophosphatidylinositol) ELISA Kit
ELK0971c 猴葡萄糖6磷酸异构酶(GPI)酶联免疫吸附测定试剂盒 Monkey GPI (Glucose 6 Phosphate Isomerase) ELISA Kit
ELK0862c 猴G蛋白偶联雌激素受体1(GPER1)酶联免疫吸附测定试剂盒 Monkey GPER1 (G Protein Coupled Estrogen Receptor 1) ELISA Kit
ELK2096c 猴磷脂酰肌醇蛋白聚糖4(GPC4)酶联免疫吸附测定试剂盒 Monkey GPC4 (Glypican 4) ELISA Kit
ELK2063c 猴磷脂酰肌醇蛋白聚糖3(GPC3)酶联免疫吸附测定试剂盒 Monkey GPC3 (Glypican 3) ELISA Kit
ELK0592c 猴磷脂酰肌醇蛋白聚糖2(GPC2)酶联免疫吸附测定试剂盒 Monkey GPC2 (Glypican 2) ELISA Kit
ELK2075c 猴磷脂酰肌醇蛋白聚糖1(GPC1)酶联免疫吸附测定试剂盒 Monkey GPC1 (Glypican 1) ELISA Kit
ELK1286c 猴高尔基蛋白73(GP-73)酶联免疫吸附测定试剂盒 Monkey GP-73 (Golgi Protein 73) ELISA Kit
ELK1640c 猴血小板膜糖蛋白VI(GP6)酶联免疫吸附测定试剂盒 Monkey GP6 (Glycoprotein VI, Platelet) ELISA Kit
ELK1135c 猴糖蛋白V(GP5)酶联免疫吸附测定试剂盒 Monkey GP5 (Glycoprotein V, Platelet) ELISA Kit
ELK0811c 猴糖蛋白49A(Gp49a)酶联免疫吸附测定试剂盒 Monkey Gp49a (Glycoprotein 49A) ELISA Kit
ELK1292c 猴血小板膜糖蛋白IV 神经细胞钾氯离子转运蛋白抗体(GP4/CD36)酶联免疫吸附测定试剂盒 Monkey GP4 (Platelet Membrane Glycoprotein IV) ELISA Kit
ELK1438c 猴软骨糖蛋白39(GP39)酶联免疫吸附测定试剂盒 Monkey GP39 (Glycoprotein 39, Cartilage) ELISA Kit
ELK1187c 猴抗糖蛋白抗体(GP)酶联免疫吸附测定试剂盒 Monkey GP (Anti-Glycoprotein Antibody) ELISA Kit
ELK1922c 猴葡萄糖氧化酶(GOD)酶联免疫吸附测定试剂盒 Monkey GOD (Glucose Oxidase) ELISA Kit
ELK1280c 猴G蛋白β1(GNβ1)酶联免疫吸附测定试剂盒 Monkey GNβ1 (G Protein Beta 1) ELISA Kit