组蛋白H3K9甲基转移酶4抗体
『别名』ERG-associated protein with SET domain; ESET; H3 K9 HMTase 4; H3 lysine 9 specific 4; H3-K9-HMTase 4; H3-K9-HMTase4; Histone H3 K9 methyltransferase 4; Histone H3-K9 methyltransferase 4; Histone lysine N methyltransferase; Histone-lysine N-methyltransferase SETDB1; KG1T; KIAA00067; KIAA0067; KMT1E; Lysine N-methyltransferase 1E; MGC90670; mKIAA0067; SET domain bifurcated 1; Set domain protein, bifurcated, 1; SETB; SETB1_HUMAN; SETDB1; Similar to DNA topoisomerase II.
『浓度』组蛋白H3K9甲基转移酶4抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
组蛋白H3K9甲基转移酶4抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
组蛋白H3K9甲基转移酶4抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
AE213Hu21 糜蛋白酶原B1(CTRB1)单克隆抗体 Monoclonal Antibody to Chymotrypsinogen B1 (CTRB1)
AG515Hu21 肥大细胞类糜蛋白酶1(CMA1)单克隆抗体 Monoclonal Antibody to Chymase 1, Mast Cell (CMA1)
AG515Ra21 肥大细胞类糜蛋白酶1(CMA1)单克隆抗体 Monoclonal Antibody to Chymase 1, Mast Cell (CMA1)
AC888Hu22 嗜铬蛋白B(CHGB)单克隆抗体 Monoclonal Antibody to Chromogranin B (CHGB)
AC888Ra21 嗜铬蛋白B(CHGB)单克隆抗体 Monoclonal Antibody to Chromogranin B (CHGB)
AB212Hu22 嗜铬蛋白A(CHGA)单克隆抗体 Monoclonal Antibody to Chromogranin A (CHGA)
AB212Ra21 嗜铬蛋白A(CHGA)单克隆抗体 Monoclonal Antibody to Chromogranin A (CHGA)
AC363Hu22 染色框同源物3(CBX3)单克隆抗体 Monoclonal Antibody to Chromobox Homolog 3 (CBX3)
AD215Hu21 绒毛膜促性腺激素β肽(CGβ)单克隆抗体 Monoclonal Antibody to Chorionic Gonadotropin Beta Polypeptide (CGb)
AB612Hu22 绒毛膜促性腺激素α肽(CGα)单克隆抗体 Monoclonal Antibody to Chorionic Gonadotropin Alpha Polypeptide (CGa)
AF625Hu21 腱蛋白样蛋白1(CHRDL1)单克隆抗体 Monoclonal Antibody to Chordin Like Protein 1 (CHRDL1)
AC126Ra21 腱蛋白(CHRD)单克隆抗体 Monoclonal Antibody to Chordin (CHRD)
AB141Ra21 硫酸软骨素蛋白多糖5(CSPG5)单克隆抗体 Monoclonal Antibody to Chondroitin Sulfate Proteoglycan 5 (CSPG5)
AD144Hu21 烟碱型胆碱受体β3(CHRNβ3)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3)
AB513Hu22 烟碱型胆碱受体β2(CHRNβ2)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2)
AB648Ra21 烟碱型胆碱受体β1(CHRNβ1)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1)
AC305Hu21 烟碱型胆碱受体α7(CHRNα7)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7)
AD142Hu21 烟碱型胆碱受体α5(CHRNα5)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5)
AD143Hu21 烟碱型胆碱受体α3(CHRNα3)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3)
AA981Hu21 烟碱型胆碱受体α1(CHRNα1)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1)
AA981Ra21 烟碱型胆碱受体α1(CHRNα1)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1)
AB838Hu21 毒蕈碱型胆碱受体M3(CHRM3)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Muscarinic 3 (CHRM3)
AB213Hu22 毒蕈碱型胆碱受体M2(CHRM2)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Muscarinic 2 (CHRM2)
AB213Ra21 毒蕈碱型胆碱受体M2(CHRM2)单克隆抗体 Monoclonal Antibody to Cholinergic Receptor, Muscarinic 2 (CHRM2)
AB929Hu21 胆碱乙酰转移酶(ChAT)单克隆抗体 Monoclonal Antibody to Choline Acetyltransferase (ChAT)
AB929Ra21 胆碱乙酰转移酶(ChAT)单克隆抗体 Monoclonal Antibody to Choline Acetyltransferase (ChAT)
AA814Hu22 脂质转运蛋白(CETP)单克隆抗体 Monoclonal Antibody to Cholesteryl Ester Transfer Protein (CETP)
AG357Hu21 胆固醇-25-羟化酶(CH25H)单克隆抗体 Monoclonal Antibody to Cholesterol-25-Hydroxylase (CH25H)
AB051Hu22 胆囊收缩素B受体(CCKBR)单克隆抗体 Monoclonal Antibody to Cholecystokinin B Receptor (CCKBR)
AA104Hu22 胆囊收缩素A受体(CCKAR)单克隆抗体 Monoclonal Antibody to Cholecystokinin A Receptor (CCKAR)
AA104Bo21 胆囊收缩素A受体(CCKAR)单克隆抗体 Monoclonal Antibody to Cholecystokinin A Receptor (CCKAR)
AA104Ca21 胆囊收缩素A受体(CCKAR)单克隆抗体 Monoclonal Antibody to Cholecystokinin A Receptor (CCKAR)
AA104Ga21 胆囊收缩素A受体(CCKAR)单克隆抗体 Monoclonal Antibody to Cholecystokinin A Receptor (CCKAR)
AA104Gu21 胆囊收缩素A受体(CCKAR)单克隆抗体 Monoclonal Antibody to Cholecystokinin A Receptor (CCKAR)
AA104Ra21 胆囊收缩素A受体(CCKAR)单克隆抗体 Monoclonal Antibody to Cholecystokinin A Receptor (CCKAR)
AA104Rb21 胆囊收缩素A受体(CCKAR)单克隆抗体 Monoclonal Antibody to Cholecystokinin A Receptor (CCKAR)
AA802Hu22 胆囊收缩素(CCK)单克隆抗体 Monoclonal Antibody to Cholecystokinin (CCK)
AA802Ca21 胆囊收缩素(CCK)单克隆抗体 Monoclonal Antibody to Cholecystokinin (CCK)
AA802Po21 胆囊收缩素(CCK)单克隆抗体 Monoclonal Antibody to Cholecystokinin (CCK)
AA802Ra21 胆囊收缩素(CCK)单克隆抗体 Monoclonal Antibody to Cholecystokinin (CCK)
AF304Hu21 氯化物细胞内通道蛋白4(CLIC4)单克隆抗体 Monoclonal Antibody to Chloride Intracellular Channel Protein 4 (CLIC4)
AC390Hu22 氯化物细胞内通道蛋白1(CLIC1)单克隆抗体 Monoclonal Antibody to Chloride Intracellular Channel Protein 1 (CLIC1)
AG669Hu21 氯离子通道辅助蛋白1(CLCA1)单克隆抗体 Monoclonal Antibody to Chloride Channel Accessory 1 (CLCA1)
AJ376Hu21 几丁质酶3样蛋白2(CHI3L2)单克隆抗体 Monoclonal Antibody to Chitinase 3 Like Protein 2 (CHI3L2)
AJ374Hu21 几丁质酶1(CHIT1)单克隆抗体 Monoclonal Antibody to Chitinase 1 (CHIT1)
AN656Hu21 趋化素样因子超家族成员6(CKLFSF6)单克隆抗体 Monoclonal Antibody to Chemokine Like Factor Superfamily 6 (CKLFSF6)
AB488Hu22 趋化因子C-X-C-基元受体7(CXCR7)单克隆抗体 Monoclonal Antibody to Chemokine C-X-C-Motif Receptor 7 (CXCR7)
AA918Hu22 趋化因子C-X-C-基元受体6(CXCR6)单克隆抗体 Monoclonal Antibody to Chemokine C-X-C-Motif Receptor 6 (CXCR6)
AA923Hu22 趋化因子C-X-C-基元受体5(CXCR5)单克隆抗体 Monoclonal Antibody to Chemokine C-X-C-Motif Receptor 5 (CXCR5)
AA940Hu22 趋化因子C-X-C-基元受体4(CXCR4)单克隆抗体 Monoclonal Antibody to Chemokine C-X-C-Motif Receptor 4 (CXCR4)
AA940Po21 趋化因子C-X-C-基元受体4(CXCR4)单克隆抗体 Monoclonal Antibody to Chemokine C-X-C-Motif Receptor 4 (CXCR4)
AA625Hu22 趋化因子C-X-C-基元受体3(CXCR3)单克隆抗体 Monoclonal Antibody to Chemokine C-X-C-Motif Receptor 3 (CXCR3)
AA625Ra21 趋化因子C-X-C-基元受体3(CXCR3)单克隆抗体 Monoclonal Antibody to Chemokine C-X-C-Motif Receptor 3 (CXCR3)
AA771Hu22 趋化因子C-X-C-基元配体16(CXCL16)单克隆抗体 Monoclonal Antibody to Chemokine C-X-C-Motif Ligand 16 (CXCL16)
AA771Ra21 趋化因子C-X-C-基元配体16(CXCL16)单克隆抗体 Monoclonal Antibody to Chemokine C-X-C-Motif Ligand 16 (CXCL16)
AB523Hu21 趋化因子C-X3-C-基元受体1(CX3CR1)单克隆抗体 Monoclonal Antibody to Chemokine C-X3-C-Motif Receptor 1 (CX3CR1)
AA040Hu22 趋化因子C-X3-C-基元配体1(CX3CL1)单克隆抗体 Monoclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
AA040Ra21 组蛋白H3K9甲基转移酶4抗体趋化因子C-X3-C-基元配体1(CX3CL1)单克隆抗体 Monoclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
AC070Hu22 趋化因子C-基元配体2(XCL2)单克隆抗体 Monoclonal Antibody to Chemokine C-Motif Ligand 2 (XCL2)
AB647Hu22 趋化因子C-C-基元受体7(CCR7)单克隆抗体 Monoclonal Antibody to Chemokine C-C-Motif Receptor 7 (CCR7)