k轻链抗体

?k轻链抗体 

『别名』HCAK 1; HCAK1; Ig kappa chain C region; IGKC; Immunoglobulin kappa constant; Immunoglobulin kappa constant region; Immunoglobulin kappa light chain; Kappa 1 immunoglobulin light chain; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809. 

『浓度』k轻链抗体1mg/1ml 

『规格』0.1ml/100μg  0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

k轻链抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

k轻链抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

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AJ851Ra21Rho鸟嘌呤核苷酸交换因子7(ARHGEF7)单克隆抗体Monoclonal Antibody to Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7)

AE330Hu21Rho GDP解离抑制因子β(ARHGDIβ)单克隆抗体Monoclonal Antibody to Rho GDP Dissociation Inhibitor Beta (ARHGDIb)

AE329Hu21Rho GDP解离抑制因子α(ARHGDIα)单克隆抗体Monoclonal Antibody to Rho GDP Dissociation Inhibitor Alpha (ARHGDIa)

AB852Hu22含Rho关联卷曲螺旋蛋白激酶2(Rock2)单克隆抗体Monoclonal Antibody to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2)

AB852Ra21含Rho关联卷曲螺旋蛋白激酶2(Rock2)单克隆抗体Monoclonal Antibody to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2)

AP729Hu21Rh血型D抗原(RHD)单克隆抗体Monoclonal Antibody to Rh Blood Group, D Antigen (RHD)

AC022Ge21反三碘甲状腺原氨酸(rT3)单克隆抗体Monoclonal Antibody to Reverse Triiodothyronine (rT3)

AM132Hu21REV1同源物(REV1)单克隆抗体Monoclonal Antibody to REV1 Homolog (REV1)

AJ847Hu21视网膜劈裂蛋白(RS)单克隆抗体Monoclonal Antibody to Retinoschisin (RS)

AP169Bo21视黄醇结合蛋白5(RBP5)单克隆抗体Monoclonal Antibody to Retinol Binding Protein 5, Cellular (RBP5)

AP169Ra21视黄醇结合蛋白5(RBP5)单克隆抗体Monoclonal Antibody to Retinol Binding Protein 5, Cellular (RBP5)

AA929Hu22视黄醇结合蛋白4(RBP4)单克隆抗体Monoclonal Antibody to Retinol Binding Protein 4, Plasma (RBP4)

AA929Bo21视黄醇结合蛋白4(RBP4)单克隆抗体Monoclonal Antibody to Retinol Binding Protein 4, Plasma (RBP4)

AA929Ra21视黄醇结合蛋白4(RBP4)单克隆抗体Monoclonal Antibody to Retinol Binding Protein 4, Plasma (RBP4)

AA367Hu22视黄醇结合蛋白3(RBP3)单克隆抗体Monoclonal Antibody to Retinol Binding Protein 3, Interstitial (RBP3)

AA377Hu21视黄醇结合蛋白2(RBP2)单克隆抗体Monoclonal Antibody to Retinol Binding Protein 2, Cellular (RBP2)

AA400Hu22视黄醇结合蛋白1(RBP1)单克隆抗体Monoclonal Antibody to Retinol Binding Protein 1, Cellular (RBP1)

AD944Hu21维甲酸X受体γ(RXRγ)单克隆抗体Monoclonal Antibody to Retinoid X Receptor Gamma (RXRg)

AC043Hu21维甲酸X受体α(RXRα)单克隆抗体Monoclonal Antibody to Retinoid X Receptor Alpha (RXRa)

AH255Hu21维甲酸受体应答基因1(RARRES1)单克隆抗体Monoclonal Antibody to Retinoic Acid Receptor Responder 1 (RARRES1)

AD952Hu21维甲酸受体γ(RARγ)单克隆抗体Monoclonal Antibody to Retinoic Acid Receptor Gamma (RARg)

AD951Hu21维甲酸受体β(RARβ)单克隆抗体Monoclonal Antibody to Retinoic Acid Receptor Beta (RARb)

AA976Hu22维甲酸受体α(RARα)单克隆抗体Monoclonal Antibody to Retinoic Acid Receptor Alpha (RARa)

AA976Ra21维甲酸受体α(RARα)单克隆抗体Monoclonal Antibody to Retinoic Acid Receptor Alpha (RARa)

AB532Hu22维甲酸诱导基因1蛋白(RIG1)单克隆抗体Monoclonal Antibody to Retinoic Acid Inducible Gene 1 Protein (RIG1)

AC776Hu21视黄醛结合蛋白1(RLBP1)单克隆抗体Monoclonal Antibody to Retinaldehyde Binding Protein 1 (RLBP1)

AF994Hu21网状蛋白4(RTN4)单克隆抗体Monoclonal Antibody to Reticulon 4 (RTN4)

AH244Hu21网钙蛋白2(RCN2)单克隆抗体Monoclonal Antibody to Reticulocalbin 2 (RCN2)

AN557Hu21补体应答基因32(RGC32)单克隆抗体Monoclonal Antibody to Response Gene To Complement 32 (RGC32)

AM131Hu21抵抗素样蛋白β(RETNLβ)单克隆抗体Monoclonal Antibody to Resistin Like Beta (RETNLb)

AA847Hu22抵抗素(RETN)单克隆抗体Monoclonal Antibody to Resistin (RETN)

AA847Bo21抵抗素(RETN)单克隆抗体Monoclonal Antibody to Resistin (RETN)

AA847Ra21抵抗素(RETN)单克隆抗体Monoclonal Antibody to Resistin (RETN)

AH217Hu21复制蛋白A1(RPA1)单克隆抗体Monoclonal Antibody to Replication Protein A1 (RPA1)

AH217Ra21复制蛋白A1(RPA1)单克隆抗体Monoclonal Antibody to Replication Protein A1 (RPA1)

AG516Hu21复制启动因子1(REPIN1)单克隆抗体Monoclonal Antibody to Replication Initiator 1 (REPIN1)

AA889Hu22肾素(REN)单克隆抗体Monoclonal Antibody to Renin (REN)

AA889Ca21肾素(REN)单克隆抗体Monoclonal Antibody to Renin (REN)

AA889Rb21肾素(REN)单克隆抗体Monoclonal Antibody to Renin (REN)

AA889Ra21肾素(REN)单克隆抗体Monoclonal Antibody to Renin (REN)

AC845Hu22肾酶(RNLS)单克隆抗体Monoclonal Antibody to Renalase (RNLS)

AC845Ra21肾酶(RNLS)单克隆抗体Monoclonal Antibody to Renalase (RNLS)

AH266Hu21肾肿瘤抗原(RAGE)单克隆抗体Monoclonal Antibody to Renal Tumor Antigen (RAGE)

AH266Ra21肾肿瘤抗原(RAGE)单克隆抗体Monoclonal Antibody to Renal Tumor Antigen (RAGE)

AD866Hu21松弛素/胰岛素样肽受体3(RXFP3)单克隆抗体Monoclonal Antibody to Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3)

AD869Ra21松弛肽3(RLN3)单克隆抗体Monoclonal Antibody to Relaxin 3 (RLN3)

AB216Hu22松弛肽(RLN)单克隆抗体Monoclonal Antibody to Relaxin (RLN)

AB216Bo21松弛肽(RLN)单克隆抗体Monoclonal Antibody to Relaxin (RLN)

AB216Po21松弛肽(RLN)单克隆抗体Monoclonal Antibody to Relaxin (RLN)

AB216Ra21松弛肽(RLN)单克隆抗体Monoclonal Antibody to Relaxin (RLN)

AB216Rb21松弛肽(RLN)单克隆抗体Monoclonal Antibody to Relaxin (RLN)

AD706Ra21蛋白信号调节因子6(RGS6)单克隆抗体Monoclonal Antibody to Regulator Of G Protein Signaling 6 (RGS6)

AP367Hu21蛋白信号调节因子19(RGS19)单克隆抗体Monoclonal Antibody to Regulator Of G Protein Signaling 19 (RGS19)

AD701Hu21蛋白信号调节因子1(RGS1)单克隆抗体Monoclonal Antibody to Regulator Of G Protein Signaling 1 (RGS1)

AA116Hu22调节激活正常T-细胞表达分泌因子(RANTES)单克隆抗体Monoclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

AA116Ca21调节激活正常T-细胞表达分泌因子(RANTES)单克隆抗体Monoclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

AA116Gu21k轻链抗体调节激活正常T-细胞表达分泌因子(RANTES)单克隆抗体Monoclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

AA116Po21调节激活正常T-细胞表达分泌因子(RANTES)单克隆抗体Monoclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

AA116Ra21调节激活正常T-细胞表达分泌因子(RANTES)单克隆抗体Monoclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

AC765Hu21钙调素(RGN)单克隆抗体Monoclonal Antibody to Regucalcin (RGN)