脊柱侧后凸畸形肽酶抗体

?脊柱侧后凸畸形肽酶抗体 

『别名』KY; KY_HUMAN; Kyphoscoliosis peptidase. 

『浓度』1mg/1ml 

『规格』脊柱侧后凸畸形肽酶抗体0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

脊柱侧后凸畸形肽酶抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

脊柱侧后凸畸形肽酶抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

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AB063Hu22 泛素结合酶E2A(UBE2A)单克隆抗体 Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2A (UBE2A)

AG945Hu21 泛素羧基端酯酶L1(UCHL1)单克隆抗体 Monoclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

AG945Ra21 泛素羧基端酯酶L1(UCHL1)单克隆抗体 Monoclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

AE593Hu21 泛素关联蛋白2(UBAP2)单克隆抗体 Monoclonal Antibody to Ubiquitin Associated Protein 2 (UBAP2)

AB027Hu22 泛素激活酶样蛋白(UBE1L)单克隆抗体 Monoclonal Antibody to Ubiquitin Activating Enzyme E1 Like Protein (UBE1L)

AE592Hu21 泛素A52残留核糖体蛋白融合产物1(UBA52)单克隆抗体 Monoclonal Antibody to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52)

AD311Hu21 泛醇细胞色素C还原酶(UQCR)单克隆抗体 Monoclonal Antibody to Ubiquinol Cytochrome C Reductase (UQCR)

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AC797Hu22 非受体酪氨酸激酶2(TNK2)单克隆抗体 Monoclonal Antibody to Tyrosine Kinase, Non Receptor 2 (TNK2)

AA125Hu22 免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)单克隆抗体 Monoclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

AB595Hu22 酪氨酸激酶2(Tyk2)单克隆抗体 Monoclonal Antibody to Tyrosine Kinase 2 (Tyk2)

AB438Hu22 酪氨酸羟化酶(TH)单克隆抗体 Monoclonal Antibody to Tyrosine Hydroxylase (TH)

AB438Ra21 酪氨酸羟化酶(TH)单克隆抗体 Monoclonal Antibody to Tyrosine Hydroxylase (TH)

AJ809Hu21 酪氨酸3/色氨酸5单加氧酶激活蛋白ζ(YWHAζ)单克隆抗体 Monoclonal Antibody to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)

AJ809Ra21 酪氨酸3/色氨酸5单加氧酶激活蛋白ζ(YWHAζ)单克隆抗体 Monoclonal Antibody to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)

AJ808Mi21 酪氨酸3/色氨酸5单加氧酶活化蛋白θ(YWHAθ)单克隆抗体 Monoclonal Antibody to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Theta (YWHAq)

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AA238Bo21 酪氨酸酶(TYR)单克隆抗体 Monoclonal Antibody to Tyrosinase (TYR)

AA238Ra21 酪氨酸酶(TYR)单克隆抗体 Monoclonal Antibody to Tyrosinase (TYR)

AG524Hu21 TYRO蛋白酪氨酸激酶结合蛋白(TYROBP)单克隆抗体 Monoclonal Antibody to TYRO Protein Tyrosine Kinase Binding Protein (TYROBP)

AF862Hu21 扭转原肠胚形成同源物1(TWSG1)单克隆抗体 Monoclonal Antibody to Twisted Gastrulation Protein Homolog 1 (TWSG1)

AC838Hu21 Twist转录因子(TWIST)单克隆抗体 Monoclonal Antibody to Twist Transcription Factor (TWIST)

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AC805Hu22 肿廇蛋白P63(TP63)单克隆抗体 Monoclonal Antibody to Tumor Protein P63 (TP63)

AH009Hu21 肿瘤蛋白p53结合蛋白1(TP53BP1)单克隆抗体 Monoclonal Antibody to Tumor Protein p53 Binding Protein 1 (TP53BP1)

AA928Hu22 肿瘤蛋白p53(TP53)单克隆抗体 Monoclonal Antibody to Tumor Protein p53 (TP53)

AA928Ra21 肿瘤蛋白p53(TP53)单克隆抗体 Monoclonal Antibody to Tumor Protein p53 (TP53)

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AA133Si21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Ca21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

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AA133Cp21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

AA133Eq21 肿瘤坏死因子α(TNFα)单克隆抗体 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

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