丝裂原活化蛋白激酶8相互作用蛋白1抗体
『别名』C jun amino terminal kinase interacting protein 1; IB 1; IB1; Islet brain 1; JIP 1; JNK interacting protein 1; JNK MAP kinase scaffold protein 1; MAPK8IP 1; MAPK8IP1; Mitogen activated protein kinase 8 interacting protein 1; PRKM8 interacting protein; PRKM8IP; JIP1_HUMAN.
『浓度』丝裂原活化蛋白激酶8相互作用蛋白1抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
丝裂原活化蛋白激酶8相互作用蛋白1抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
丝裂原活化蛋白激酶8相互作用蛋白1抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAD740Hu01 载脂蛋白A1结合蛋白(APOA1BP)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 Binding Protein (APOA1BP)
PAA519Hu01 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA519Mu01 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA519Ra01 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA519Rb51 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA519Po01 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA519Bo01 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA519Cp01 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA519Ca01 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA519Mu02 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA519Si01 载脂蛋白A1(APOA1)多克隆抗体 Polyclonal Antibody to Apolipoprotein A1 (APOA1)
PAA426Hu01 Apobec 1偶联因子(ACF)多克隆抗体 Polyclonal Antibody to Apobec 1 Complementation Factor (ACF)
PAK040Mu01 APEX核酸酶1(APEX1)多克隆抗体 Polyclonal Antibody to APEX Nuclease 1 (APEX1)
PAK040Mu02 APEX核酸酶1(APEX1)多克隆抗体 Polyclonal Antibody to APEX Nuclease 1 (APEX1)
PAD067Ra01 爱帕琳肽受体(APLNR)多克隆抗体 Polyclonal Antibody to Apelin Receptor (APLNR)
PAA856Hu01 爱帕琳肽12(AP12)多克隆抗体 Polyclonal Antibody to Apelin 12 (AP12)
PAD066Hu01 爱帕琳肽(APLN)多克隆抗体 Polyclonal Antibody to Apelin (APLN)
PAD066Ra01 爱帕琳肽(APLN)多克隆抗体 Polyclonal Antibody to Apelin (APLN)
PAD066Hu02 爱帕琳肽(APLN)多克隆抗体 Polyclonal Antibody to Apelin (APLN)
PAD066Mu01 爱帕琳肽(APLN)多克隆抗体 Polyclonal Antibody to Apelin (APLN)
PAD066Mu02 爱帕琳肽(APLN)多克隆抗体 Polyclonal Antibody to Apelin (APLN)
PAD066Hu03 爱帕琳肽(APLN)多克隆抗体 Polyclonal Antibody to Apelin (APLN)
PAC313Hu01 抗凝血酶(AT)多克隆抗体 Polyclonal Antibody to Antithrombin (AT)
PAC313Mu01 抗凝血酶(AT)多克隆抗体 Polyclonal Antibody to Antithrombin (AT)
PAC313Ra01 抗凝血酶(AT)多克隆抗体 Polyclonal Antibody to Antithrombin (AT)
PAC313Mu02 抗凝血酶(AT)多克隆抗体 Polyclonal Antibody to Antithrombin (AT)
PAA228Ga01 抗缪勒管激素(AMH)多克隆抗体 Polyclonal Antibody to Anti-Mullerian Hormone (AMH)
PAA228Hu01 抗缪勒管激素(AMH)多克隆抗体 Polyclonal Antibody to Anti-Mullerian Hormone (AMH)
PAA228Mu01 抗缪勒管激素(AMH)多克隆抗体 Polyclonal Antibody to Anti-Mullerian Hormone (AMH)
PAA228Po01 抗缪勒管激素(AMH)多克隆抗体 Polyclonal Antibody to Anti-Mullerian Hormone (AMH)
PAA228Ra01 抗缪勒管激素(AMH)多克隆抗体 Polyclonal Antibody to Anti-Mullerian Hormone (AMH)
PAA228Bo01 抗缪勒管激素(AMH)多克隆抗体 Polyclonal Antibody to Anti-Mullerian Hormone (AMH)
PAA228Hu02 抗缪勒管激素(AMH)多克隆抗体 Polyclonal Antibody to Anti-Mullerian Hormone (AMH)
PAD113Hu01 抗原肽转运蛋白1(TAP1)多克隆抗体 Polyclonal Antibody to Antigen Peptide Transporter 1 (TAP1)
PAB139Hu01 抗利尿激素(ADH)多克隆抗体 Polyclonal Antibody to Antidiuretic Hormone (ADH)
PAF212Hu01 炭疽热毒素受体2(ANTXR2)多克隆抗体 Polyclonal Antibody to Anthrax Toxin Receptor 2 (ANTXR2)
PAC285Hu01 前梯度蛋白2(AGR2)多克隆抗体 Polyclonal Antibody to Anterior Gradient Protein 2 (AGR2)
PAC285Mu01 前梯度蛋白2(AGR2)多克隆抗体 Polyclonal Antibody to Anterior Gradient Protein 2 (AGR2)
PAC285Ra01 前梯度蛋白2(AGR2)多克隆抗体 Polyclonal Antibody to Anterior Gradient Protein 2 (AGR2)
PAF813Hu01 钙激活氯通道蛋白6(ANO6)多克隆抗体 Polyclonal Antibody to Anoctamin 6 (ANO6)
PAB384Mu01 钙激活氯通道蛋白1(ANO1)多克隆抗体 Polyclonal Antibody to Anoctamin 1 (ANO1)
PAE779Hu01 膜联蛋白A9(ANXA9)多克隆抗体 Polyclonal Antibody to Annexin A9 (ANXA9)
PAC345Hu01 膜联蛋白A6(ANXA6)多克隆抗体 Polyclonal Antibody to Annexin A6 (ANXA6)
PAC345Mu01 膜联蛋白A6(ANXA6)多克隆抗体 Polyclonal Antibody to Annexin A6 (ANXA6)
PAA259Bo01 膜联蛋白A5(ANXA5)多克隆抗体 Polyclonal Antibody to Annexin A5 (ANXA5)
PAA259Ga01 膜联蛋白A5(ANXA5)多克隆抗体 Polyclonal Antibody to Annexin A5 (ANXA5)
PAA259Hu01 膜联蛋白A5(ANXA5)多克隆抗体 Polyclonal Antibody to Annexin A5 (ANXA5)
PAA259Mu01 膜联蛋白A5(ANXA5)多克隆抗体 Polyclonal Antibody to Annexin A5 (ANXA5)
PAA259Ra01 膜联蛋白A5(ANXA5)多克隆抗体 Polyclonal Antibody to Annexin A5 (ANXA5)
PAA259Si01 膜联蛋白A5(ANXA5)多克隆抗体 Polyclonal Antibody to Annexin A5 (ANXA5)
PAA259Mu02 膜联蛋白A5(ANXA5)多克隆抗体 Polyclonal Antibody to Annexin A5 (ANXA5)
PAE784Hu01 膜联蛋白A4(ANXA4)多克隆抗体 Polyclonal Antibody to Annexin A4 (ANXA4)
PAE784Ca01 膜联蛋白A4(ANXA4)多克隆抗体 Polyclonal Antibody to Annexin A4 (ANXA4)
PAE784Mu01 膜联蛋白A4(ANXA4)多克隆抗体 Polyclonal Antibody to Annexin A4 (ANXA4)
PAE784Po01 膜联蛋白A4(ANXA4)多克隆抗体 Polyclonal Antibody to Annexin A4 (ANXA4)
PAE784Ra01 膜联蛋白A4丝裂原活化蛋白激酶8相互作用蛋白1抗体(ANXA4)多克隆抗体 Polyclonal Antibody to Annexin A4 (ANXA4)
PAE784Mu02 膜联蛋白A4(ANXA4)多克隆抗体 Polyclonal Antibody to Annexin A4 (ANXA4)
PAE784Ra02 膜联蛋白A4(ANXA4)多克隆抗体 Polyclonal Antibody to Annexin A4 (ANXA4)
PAE784Po02 膜联蛋白A4(ANXA4)多克隆抗体 Polyclonal Antibody to Annexin A4 (ANXA4)