JNK相互作用蛋白4抗体
『别名』C jun amino terminal kinase interacting protein 4; c Jun NH2 terminal kinase associated leucine zipper protein; FLJ13450; FLJ14006; FLJ34602; HLC 4; HLC 4 protein; HLC 6; HLC4; HLC4 protein; HLC6; HSS; Human lung cancer protein 6; Human lung cancer protein 6; JIP 4; JIP 4; JIP4; JNK associated leucine zipper protein 1; JNK associated leucine zipper protein; JNK interacting protein 4; JNK interacting protein 4; JNK interacting protein; JNK/SAPK associated protein; KIAA0516; Lung cancer oncogene 4; JNK相互作用蛋白4抗体MAPK8IP4; Max binding protein; MGC117291; MGC14967; MGC74461; Mitogen activated protein kinase 8 interacting protein 4; Mitogen activated protein kinase 8 interacting protein 4; PHET; PIG 6; PIG6; Proliferation inducing gene 6; Protein highly expressed in testis; SPAG 9; SPAG9; Sperm associated antigen 9; Sperm specific protein; Sperm surface protein; Sunday driver (drosophila) homolog; Sunday driver 1; SYD1; JIP4_HUMAN.
『浓度』1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
JNK相互作用蛋白4抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
JNK相互作用蛋白4抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAG371Mu01 钠/钾离子转运ATP酶β1肽(ATP1β1)多克隆抗体 Polyclonal Antibody to ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1)
PAG371Mu02 钠/钾离子转运ATP酶β1肽(ATP1β1)多克隆抗体 Polyclonal Antibody to ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1)
PAG371Ra01 钠/钾离子转运ATP酶β1肽(ATP1β1)多克隆抗体 Polyclonal Antibody to ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1)
PAG368Mi01 钠/钾离子转运ATP酶α2肽(ATP1α2)多克隆抗体 Polyclonal Antibody to ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2)
PAG369Rb51 钠/钾离子转运ATP酶α1肽(ATP1α1)多克隆抗体 Polyclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)
PAG369Rb52 钠/钾离子转运ATP酶α1肽(ATP1α1)多克隆抗体 Polyclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)
PAG369Mi01 钠/钾离子转运ATP酶α1肽(ATP1α1)多克隆抗体 Polyclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)
PAG384Mu01 氢/钾离子交换ATP酶β肽(ATP4β)多克隆抗体 Polyclonal Antibody to ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b)
PAG384Mu02 氢/钾离子交换ATP酶β肽(ATP4β)多克隆抗体 Polyclonal Antibody to ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b)
PAG383Mu01 氢/钾离子交换ATP酶α肽(ATP4α)多克隆抗体 Polyclonal Antibody to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
PAG383Hu01 氢/钾离子交换ATP酶α肽(ATP4α)多克隆抗体 Polyclonal Antibody to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
PAG383Mu02 氢/钾离子交换ATP酶α肽(ATP4α)多克隆抗体 Polyclonal Antibody to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
PAG387Ra01 氢离子转运ATP酶线粒体F1复合体β肽(ATP5β)多克隆抗体 Polyclonal Antibody to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b)
PAG393Ra01 氢离子转运ATP酶溶酶体辅助蛋白2(ATP6AP2)多克隆抗体 Polyclonal Antibody to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2)
PAG393Hu01 氢离子转运ATP酶溶酶体辅助蛋白2(ATP6AP2)多克隆抗体 Polyclonal Antibody to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2)
PAG386Hu01 铜离子转运ATP酶β肽(ATP7β)多克隆抗体 Polyclonal Antibody to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b)
PAG386Mu01 铜离子转运ATP酶β肽(ATP7β)多克隆抗体 Polyclonal Antibody to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b)
PAG386Mu02 铜离子转运ATP酶β肽(ATP7β)多克隆抗体 Polyclonal Antibody to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b)
PAG377Mu01 钙离子转运ATP酶B2(ATP2B2)多克隆抗体 Polyclonal Antibody to ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2)
PAG377Mu02 钙离子转运ATP酶B2(ATP2B2)多克隆抗体 Polyclonal Antibody to ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2)
PAG374Hu01 钙离子转运ATP酶A2(ATP2A2)多克隆抗体 Polyclonal Antibody to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)
PAG374Mu01 钙离子转运ATP酶A2(ATP2A2)多克隆抗体 Polyclonal Antibody to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)
PAG374Mu02 钙离子转运ATP酶A2(ATP2A2)多克隆抗体 Polyclonal Antibody to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)
PAB269Ra01 ATP依赖性DNA连接酶Ⅰ(LIG1)多克隆抗体 Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1)
PAA960Hu01 ATP结合盒转运蛋白G2(ABCG2)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter G2 (ABCG2)
PAA960Mu01 ATP结合盒转运蛋白G2(ABCG2)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter G2 (ABCG2)
PAA960Mu02 ATP结合盒转运蛋白G2(ABCG2)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter G2 (ABCG2)
PAB288Hu01 ATP结合盒转运蛋白G1(ABCG1)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter G1 (ABCG1)
PAD629Hu01 ATP结合盒转运蛋白C9(ABCC9)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C9 (ABCC9)
PAD628Hu01 ATP结合盒转运蛋白C8(ABCC8)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C8 (ABCC8)
PAD626Hu01 ATP结合盒转运蛋白C6(ABCC6)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C6 (ABCC6)
PAD626Mu01 ATP结合盒转运蛋白C6(ABCC6)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C6 (ABCC6)
PAD626Mu02 ATP结合盒转运蛋白C6(ABCC6)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C6 (ABCC6)
PAD625Hu01 ATP结合盒转运蛋白C5(ABCC5)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C5 (ABCC5)
PAD624Hu01 ATP结合盒转运蛋白C4(ABCC4)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C4 (ABCC4)
PAD623Hu01 ATP结合盒转运蛋白C3(ABCC3)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C3 (ABCC3)
PAD622Hu01 ATP结合盒转运蛋白C2(ABCC2)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C2 (ABCC2)
PAD633Hu01 ATP结合盒转运蛋白C13(ABCC13)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C13 (ABCC13)
PAD632Hu01 ATP结合盒转运蛋白C12(ABCC12)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C12 (ABCC12)
PAD631Hu01 ATP结合盒转运蛋白C11(ABCC11)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C11 (ABCC11)
PAQ209Hu01 ATP结合盒转运蛋白C10(ABCC10)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter C10 (ABCC10)
PAD635Hu01 ATP结合盒转运蛋白B5(ABCB5)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter B5 (ABCB5)
PAD641Mu01 ATP结合盒转运蛋白B11(ABCB11)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter B11 (ABCB11)
PAD620Hu01 ATP结合盒转运蛋白A4(ABCA4)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter A4 (ABCA4)
PAD619Hu01 ATP结合盒转运蛋白A3(ABCA3)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter A3 (ABCA3)
PAD651Mu01 ATP结合盒转运蛋白A13(ABCA13)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter A13 (ABCA13)
PAD651Mu02 ATP结合盒转运蛋白A13(ABCA13)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter A13 (ABCA13)
PAC521Hu01 ATP结合盒转运蛋白A12(ABCA12)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter A12 (ABCA12)
PAB242Hu01 ATP结合盒转运蛋白A1(ABCA1)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter A1 (ABCA1)
PAB242Mu01 ATP结合盒转运蛋白A1(ABCA1)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter A1 (ABCA1)
PAB242Mu02 ATP结合盒转运蛋白A1(ABCA1)多克隆抗体 Polyclonal Antibody to ATP Binding Cassette Transporter A1 (ABCA1)
PAB557Ra01 失调蛋白2结合蛋白1JNK相互作用蛋白4抗体(A2BP1)多克隆抗体 Polyclonal Antibody to Ataxin 2 Binding Protein 1 (A2BP1)
PAD248Mu01 失调蛋白10(ATXN10)多克隆抗体 Polyclonal Antibody to Ataxin 10 (ATXN10)
PAD248Mu02 失调蛋白10(ATXN10)多克隆抗体 Polyclonal Antibody to Ataxin 10 (ATXN10)
PAD248Hu01 失调蛋白10(ATXN10)多克隆抗体 Polyclonal Antibody to Ataxin 10 (ATXN10)