NF-kappa-B抑制子beta抗体
『别名』I kappa B beta; I-kappa-B-beta; IkappaBbeta; IKB beta; IkB-B; IkB-beta; IKBB; IKBB_HUMAN; IkBbeta; NF kappa BIB; NF-kappa-B inhibitor beta; NF-kappa-BIB; Nfkbib; Thyroid receptor interacting protein 9; Thyroid receptor-interacting protein 9; TR interacting protein 9; TR-interacting protein 9; TRIP-9; TRIP9.
『浓度』NF-kappa-B抑制子beta抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
NF-kappa-B抑制子beta抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
NF-kappa-B抑制子beta抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA541Ca01 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)
PAA541Mu02 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)
PAA541Hu02 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)
PAA541Mu03 脑钠素(BNP)多克隆抗体 Polyclonal Antibody to Brain Natriuretic Peptide (BNP)
PAB093Mu01 脑指蛋白(BFP)多克隆抗体 Polyclonal Antibody to Brain Finger Protein (BFP)
PAB093Mu02 脑指蛋白(BFP)多克隆抗体 Polyclonal Antibody to Brain Finger Protein (BFP)
PAB093Ra01 脑指蛋白(BFP)多克隆抗体 Polyclonal Antibody to Brain Finger Protein (BFP)
PAB093Hu01 脑指蛋白(BFP)多克隆抗体 Polyclonal Antibody to Brain Finger Protein (BFP)
PAA011Bo01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Ca01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Eq01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Ga01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Gu01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Hu01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Mu01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Po01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Ra01 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Rb52 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Rb51 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAA011Mu02 脑源性神经营养因子(BDNF)多克隆抗体 Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
PAJ563Hu01 脑富含膜附着信号蛋白1(BASP1)多克隆抗体 Polyclonal Antibody to Brain Abundant, Membrane Attached Signal Protein 1 (BASP1)
PAG347Hu01 B-Raf原癌基因丝氨酸/苏氨酸蛋白激酶(BRAF)多克隆抗体 Polyclonal Antibody to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)
PAC344Ra01 缓激肽受体B2(BDKRB2)多克隆抗体 Polyclonal Antibody to Bradykinin Receptor B2 (BDKRB2)
PAA874Hu01 缓激肽(BK)多克隆抗体 Polyclonal Antibody to Bradykinin (BK)
PAA248Ge01 牛血清白蛋白(BSA)多克隆抗体 Polyclonal Antibody to Bovine Serum Albumin (BSA)
PAB092Hu01 骨涎蛋白(BSP)多克隆抗体 Polyclonal Antibody to Bone Sialoprotein (BSP)
PAB092Mu01 骨涎蛋白(BSP)多克隆抗体 Polyclonal Antibody to Bone Sialoprotein (BSP)
PAB092Mu02 骨涎蛋白(BSP)多克隆抗体 Polyclonal Antibody to Bone Sialoprotein (BSP)
PAA016Hu01 骨成型蛋白受体2(BMPR2)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein Receptor 2 (BMPR2)
PAA730Hu01 骨成型蛋白受体1B(BMPR1B)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein Receptor 1B (BMPR1B)
PAA015Hu01 骨成型蛋白受体1A(BMPR1A)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein Receptor 1A (BMPR1A)
PAA015Mu01 骨成型蛋白受体1A(BMPR1A)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein Receptor 1A (BMPR1A)
PAA015Ra01 骨成型蛋白受体1A(BMPR1A)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein Receptor 1A (BMPR1A)
PAA015Bo01 骨成型蛋白受体1A(BMPR1A)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein Receptor 1A (BMPR1A)
PAA015Mu02 骨成型蛋白受体1A(BMPR1A)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein Receptor 1A (BMPR1A)
PAC105Mu01 骨形成蛋白8B(BMP8B)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 8B (BMP8B)
PAC105Mu02 骨形成蛋白8B(BMP8B)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 8B (BMP8B)
PAC105Hu01 骨形成蛋白8B(BMP8B)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 8B (BMP8B)
PAC104Ra01 骨形成蛋白8A(BMP8A)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 8A (BMP8A)
PAC104Hu01 骨形成蛋白8A(BMP8A)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 8A (BMP8A)
PAA799Hu01 骨成型蛋白7(BMP7)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 7 (BMP7)
PAA799Mu01 骨成型蛋白7(BMP7)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 7 (BMP7)
PAA799Ra01 骨成型蛋白7(BMP7)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 7 (BMP7)
PAA799Mu02 骨成型蛋白7(BMP7)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 7 (BMP7)
PAA799Bo01 骨成型蛋白7(BMP7)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 7 (BMP7)
PAA799Hu02 骨成型蛋白7(BMP7)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 7 (BMP7)
PAA646Hu01 骨形成蛋白6(BMP6)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 6 (BMP6)
PAA646Mu01 骨形成蛋白6(BMP6)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 6 (BMP6)
PAA646Ra01 骨形成蛋白6(BMP6)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 6 (BMP6)
PAA646Mu02 骨形成蛋白6(BMP6)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 6 (BMP6)
PAC103Hu01 骨成型蛋白5(BMP5)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 5 (BMP5)
PAC103Mu01 骨成型蛋白5(BMP5)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 5 (BMP5)
PAC103Mu02 骨成型蛋白5(BMP5)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 5 (BMP5)
PAA014Hu01 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Mu01 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Ra01 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Bo01 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Ca01 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Cp01 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Ga01 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Po01 骨成型蛋白4NF-kappa-B抑制子beta抗体(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Rb53 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Rb51 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)
PAA014Rb52 骨成型蛋白4(BMP4)多克隆抗体 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4)