细胞纤毛内转运同源蛋白122抗体

细胞纤毛内转运同源蛋白122抗体 

『别名』CED; IF122_HUMAN; IFT122; Intraflagellar transport 122 homolog (Chlamydomonas); 细胞纤毛内转运同源蛋白122抗体Intraflagellar transport protein 122 homolog; SPG; WD repeat domain 10; WD repeat-containing protein 10; WD repeat-containing protein 140; WDR10; WDR10p; WDR140. 

『浓度』1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

细胞纤毛内转运同源蛋白122抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

细胞纤毛内转运同源蛋白122抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAA042Po01 粒细胞集落刺激因子(GCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

PAA042Mu02 粒细胞集落刺激因子(GCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

PAA042Bo01 粒细胞集落刺激因子(GCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

PAA045Ca01 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAA045Hu01 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAA045Mu01 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAA045Ra01 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAA045Mu02 粒细胞巨噬细胞集落刺激因子(GMCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

PAB535Hu01 集落刺激因子2受体β(CSF2Rβ)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb)

PAB487Mu01 集落刺激因子2受体α(CSF2Rα)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra)

PAB487Hu01 集落刺激因子2受体α(CSF2Rα)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra)

PAB487Hu02 集落刺激因子2受体α(CSF2Rα)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra)

PAA090Hu01 巨噬细胞集落刺激因子(MCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 1, Macrophage (MCSF)

PAA090Mu01 巨噬细胞集落刺激因子(MCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 1, Macrophage (MCSF)

PAA090Ra01 巨噬细胞集落刺激因子(MCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 1, Macrophage (MCSF)

PAA090Mu02 巨噬细胞集落刺激因子(MCSF)多克隆抗体 Polyclonal Antibody to Colony Stimulating Factor 1, Macrophage (MCSF)

PAB502Hu01 肝胶原凝集素1(CLL1)多克隆抗体 Polyclonal Antibody to Collectin Liver 1 (CLL1)

PAC134Mu01 ⅩⅧ型胶原(COL18)多克隆抗体 Polyclonal Antibody to Collagen Type XVIII (COL18)

PAC134Ra01 ⅩⅧ型胶原(COL18)多克隆抗体 Polyclonal Antibody to Collagen Type XVIII (COL18)

PAC134Hu01 ⅩⅧ型胶原(COL18)多克隆抗体 Polyclonal Antibody to Collagen Type XVIII (COL18)

PAC135Hu01 ⅩⅦ型胶原(COL17)多克隆抗体 Polyclonal Antibody to Collagen Type XVII (COL17)

PAC136Hu01 ⅩⅥ型胶原(COL16)多克隆抗体 Polyclonal Antibody to Collagen Type XVI (COL16)

PAC137Hu01 ⅩⅤ型胶原(COL15)多克隆抗体 Polyclonal Antibody to Collagen Type XV (COL15)

PAC137Mu01 ⅩⅤ型胶原(COL15)多克隆抗体 Polyclonal Antibody to Collagen Type XV (COL15)

PAC137Mu02 ⅩⅤ型胶原(COL15)多克隆抗体 Polyclonal Antibody to Collagen Type XV (COL15)

PAC133Ra01 ⅩⅨ型胶原(COL19)多克隆抗体 Polyclonal Antibody to Collagen Type XIX (COL19)

PAD133Hu01 ⅩⅣ型胶原(COL14)多克隆抗体 Polyclonal Antibody to Collagen Type XIV (COL14)

PAC138Hu01 ⅩⅢ型胶原(COL13)多克隆抗体 Polyclonal Antibody to Collagen Type XIII (COL13)

PAC158Hu01 Ⅻ型胶原(COL12)多克隆抗体 Polyclonal Antibody to Collagen Type XII (COL12)

PAC158Mu01 Ⅻ型胶原(COL12)多克隆抗体 Polyclonal Antibody to Collagen Type XII (COL12)

PAC158Mu02 Ⅻ型胶原(COL12)多克隆抗体 Polyclonal Antibody to Collagen Type XII (COL12)

PAC158Ra01 Ⅻ型胶原(COL12)多克隆抗体 Polyclonal Antibody to Collagen Type XII (COL12)

PAC140Hu01 Ⅺ型胶原α2(COL11α2)多克隆抗体 Polyclonal Antibody to Collagen Type XI Alpha 2 (COL11a2)

PAC157Hu01 Ⅺ型胶原α1(COL11α1)多克隆抗体 Polyclonal Antibody to Collagen Type XI Alpha 1 (COL11a1)

PAC156Hu01 Ⅹ型胶原(COL10)多克隆抗体 Polyclonal Antibody to Collagen Type X (COL10)

PAC156Mu01 Ⅹ型胶原(COL10)多克隆抗体 Polyclonal Antibody to Collagen Type X (COL10)

PAC156Ra01 Ⅹ型胶原(COL10)多克隆抗体 Polyclonal Antibody to Collagen Type X (COL10)

PAC156Mu02 Ⅹ型胶原(COL10)多克隆抗体 Polyclonal Antibody to Collagen Type X (COL10)

PAC156Bo01 Ⅹ型胶原(COL10)多克隆抗体 Polyclonal Antibody to Collagen Type X (COL10)

PAD124Hu01 Ⅷ型胶原α2(COL8α2)多克隆抗体 Polyclonal Antibody to Collagen Type VIII Alpha 2 (COL8a2)

PAD124Mu01 Ⅷ型胶原α2(COL8α2)多克隆抗体 Polyclonal Antibody to Collagen Type VIII Alpha 2 (COL8a2)

PAC146Hu01 Ⅷ型胶原α1(COL8α1)多克隆抗体 Polyclonal Antibody to Collagen Type VIII Alpha 1 (COL8a1)

PAC146Mu01 Ⅷ型胶原α1(COL8α1)多克隆抗体 Polyclonal Antibody to Collagen Type VIII Alpha 1 (COL8a1)

PAC146Ra01 Ⅷ型胶原α1(COL8α1)多克隆抗体 Polyclonal Antibody to Collagen Type VIII Alpha 1 (COL8a1)

PAC146Rb51 Ⅷ型胶原α1(COL8α1)多克隆抗体 Polyclonal Antibody to Collagen Type VIII Alpha 1 (COL8a1)

PAC147Hu01 Ⅶ型胶原(COL7)多克隆抗体 Polyclonal Antibody to Collagen Type VII (COL7)

PAC147Mu01 Ⅶ型胶原(COL7)多克隆抗体 Polyclonal Antibody to Collagen Type VII (COL7)

PAC147Mu02 Ⅶ型胶原(COL7)多克隆抗体 Polyclonal Antibody to Collagen Type VII (COL7)

PAC148Hu01 Ⅵ型胶原α3(COL6α3)多克隆抗体 Polyclonal Antibody to Collagen Type VI Alpha 3 (COL6a3)

PAC150Hu01 Ⅵ型胶原α1(COL6α1)多克隆抗体 Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)

PAC150Mu01 Ⅵ型胶原α1(COL6α1)多克隆抗体 Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)

PAC150Hu02 Ⅵ型胶原α1(COL6α1)多克隆抗体 Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)

PAC150Mu03 Ⅵ型胶原α1(COL6α1)多克隆抗体 Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)

PAC150Mu02 Ⅵ型胶原α1(COL6α1)多克隆抗体 Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)

PAC150Ra01 Ⅵ型胶原α1(COL6α1)多克隆抗体 Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)

PAC152Hu01 Ⅴ型胶原α2(COL5α2)多克隆抗体 Polyclonal Antibody to Collagen Type V Alpha 2 (COL5a2)

PAC152Mu01 Ⅴ型胶原α2细胞纤毛内转运同源蛋白122抗体(COL5α2)多克隆抗体 Polyclonal Antibody to Collagen Type V Alpha 2 (COL5a2)

PAC152Mu02 Ⅴ型胶原α2(COL5α2)多克隆抗体 Polyclonal Antibody to Collagen Type V Alpha 2 (COL5a2)

PAC155Hu01 Ⅸ型胶原α3(COL9α3)多克隆抗体 Polyclonal Antibody to Collagen Type IX Alpha 3 (COL9a3)

PAC145Ra01 Ⅸ型胶原α1(COL9α1)多克隆抗体 Polyclonal Antibody to Collagen Type IX Alpha 1 (COL9a1)

PAC141Hu01 Ⅳ型胶原α5(COL4α5)多克隆抗体 Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)