干扰素诱导的三角形四肽重复蛋白2抗体
『别名』CIG-42; cig42; G10P2; GARG 39; Glucocorticoid attenuated response gene 39; IFI 54; IFI 54K; IFI-54K; IFI54; IFIT 2; IFIT-2; IFIT2; IFIT2_HUMAN; Interferon induced 54 kDa protein; Interferon induced protein 54; Interferon induced protein with tetratricopeptide repeats 2; Interferon, alpha inducible protein (MW 54kD); Interferon-induced 54 kDa protein; Interferon-induced protein with tetratricopeptide repeats 2; ISG 54 K; ISG 54K; ISG-54 K; ISG54.
『浓度』干扰素诱导的三角形四肽重复蛋白2抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
干扰素诱导的三角形四肽重复蛋白2抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
干扰素诱导的三角形四肽重复蛋白2抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAC042Hu01 法尼酯X受体(FXR)多克隆抗体 Polyclonal Antibody to Farnesoid X Receptor (FXR)
PAC042Mu01 法尼酯X受体(FXR)多克隆抗体 Polyclonal Antibody to Farnesoid X Receptor (FXR)
PAC042Mu02 法尼酯X受体(FXR)多克隆抗体 Polyclonal Antibody to Farnesoid X Receptor (FXR)
PAC042Ra01 法尼酯X受体(FXR)多克隆抗体 Polyclonal Antibody to Farnesoid X Receptor (FXR)
PAR030Hu01 序列相似家族5成员C(FAM5C)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 5, Member C (FAM5C)
PAK368Hu01 序列相似家族3成员D(FAM3D)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member D (FAM3D)
PAL506Hu01 序列相似家族3成员C(FAM3C)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member C (FAM3C)
PAL507Hu01 序列相似家族3成员B(FAM3B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member B (FAM3B)
PAL507Mu01 序列相似家族3成员B(FAM3B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member B (FAM3B)
PAL507Mu02 序列相似家族3成员B(FAM3B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 3, Member B (FAM3B)
PAK371Hu01 序列相似家族20成员A(FAM20A)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 20, Member A (FAM20A)
PAQ976Hu01 序列相似家族19成员A4(FAM19A4)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 19, Member A4 (FAM19A4)
PAQ975Hu01 序列相似家族19成员A3(FAM19A3)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 19, Member A3 (FAM19A3)
PAQ975Mu01 序列相似家族19成员A3(FAM19A3)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 19, Member A3 (FAM19A3)
PAQ974Mu01 序列相似家族19成员A2(FAM19A2)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 19, Member A2 (FAM19A2)
PAR758Mu01 序列相似家族135成员B(FAM135B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 135, Member B (FAM135B)
PAR758Mu02 序列相似家族135成员B(FAM135B)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 135, Member B (FAM135B)
PAV122Hu01 序列相似家族132成员A(FAM132A)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 132, Member A (FAM132A)
PAV122Hu02 序列相似家族132成员A(FAM132A)多克隆抗体 Polyclonal Antibody to Family With Sequence Similarity 132, Member A (FAM132A)
PAA031Hu01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)
PAA031Mu01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)
PAA031Po01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)
PAA031Ra01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)
PAA031Si01 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)
PAA031Hu02 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)
PAA031Mu02 凋亡相关因子配体(FASL)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL)
PAA030Bo01 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Hu01 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Mu01 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Po01 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Ra01 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Si01 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Hu02 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Mu02 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Ra02 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Hu03 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA030Ra03 凋亡相关因子(FAS)多克隆抗体 Polyclonal Antibody to Factor Related Apoptosis (FAS)
PAA930Hu01 细胞外信号调节激酶2(ERK2)多克隆抗体 Polyclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2)
PAA930Ra01 细胞外信号调节激酶2(ERK2)多克隆抗体 Polyclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2)
PAB357Hu01 细胞外信号调节激酶1(ERK1)多克隆抗体 Polyclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)
PAB357Ra01 细胞外信号调节激酶1(ERK1)多克隆抗体 Polyclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)
PAC450Hu01 细胞外基质蛋白1(ECM1)多克隆抗体 Polyclonal Antibody to Extracellular Matrix Protein 1 (ECM1)
PAC450Mu01 细胞外基质蛋白1(ECM1)多克隆抗体 Polyclonal Antibody to Extracellular Matrix Protein 1 (ECM1)
PAC450Mu02 细胞外基质蛋白1(ECM1)多克隆抗体 Polyclonal Antibody to Extracellular Matrix Protein 1 (ECM1)
PAB540Hu01 细胞外基质金属蛋白酶诱导因子(EMMPRIN)多克隆抗体 Polyclonal Antibody to Extracellular Matrix Metalloproteinase Inducer (EMMPRIN)
PAB540Ra01 细胞外基质金属蛋白酶诱导因子(EMMPRIN)多克隆抗体 Polyclonal Antibody to Extracellular Matrix Metalloproteinase Inducer (EMMPRIN)
PAB540Mu01 细胞外基质金属蛋白酶诱导因子(EMMPRIN)多克隆抗体 Polyclonal Antibody to Extracellular Matrix Metalloproteinase Inducer (EMMPRIN)
PAF160Hu01 输出蛋白5(XPO5)多克隆抗体 Polyclonal Antibody to Exportin 5 (XPO5)
PAC258Hu01 输出蛋白1(XPO1)多克隆抗体 Polyclonal Antibody to Exportin 1 (XPO1)
PAL477Hu01 外生骨疣样蛋白1(EXTL1)多克隆抗体 Polyclonal Antibody to Exostoses Like Protein 1 (EXTL1)
PAG239Hu01 外生骨疣蛋白1(EXT1)多克隆抗体 Polyclonal Antibody to Exostoses 1 (EXT1)
PAH822Hu01 外切酶体成分2(EXOSC2)多克隆抗体 Polyclonal Antibody to Exosome Component 2 (EXOSC2)
PAG552Hu01 兴奋性氨基酸转运蛋白5(EAAT5)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 5 (EAAT5)
PAG553Hu01 兴奋性氨基酸转运蛋白4(EAAT4)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 4 (EAAT4)
PAE342Hu01 兴奋性氨基酸转运蛋白3(EAAT3)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 3 (EAAT3)
PAE346Mu01 兴奋性氨基酸转运蛋白2(EAAT2)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 2 (EAAT2)
PAE346Hu01 兴奋性氨基酸转运蛋白2(EAAT2)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 2 (EAAT2)
PAE346Mu02 兴奋性氨基酸转运蛋白2(EAAT2)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 2 (EAAT2)
PAE346Ra01 兴奋性氨基酸转运蛋白2(EAAT2)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 2干扰素诱导的三角形四肽重复蛋白2抗体 (EAAT2)
PAE343Hu01 兴奋性氨基酸转运蛋白1(EAAT1)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 1 (EAAT1)
PAE343Mu01 兴奋性氨基酸转运蛋白1(EAAT1)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 1 (EAAT1)
PAE343Mu02 兴奋性氨基酸转运蛋白1(EAAT1)多克隆抗体 Polyclonal Antibody to Excitatory Amino Acid Transporter 1 (EAAT1)