胰岛素抗体

胰岛素抗体 

『别名』ILPR; INS; Insulin A chain; Insulin B chain; Insulin A chain; Insulin precursor; IRDN; Proinsulin; Proinsulin precursor; IDDM2; INS_HUMAN; MODY10. 

『浓度』胰岛素抗体1mg/1ml 

『规格』0.1ml/100μg  0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

胰岛素抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

胰岛素抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAA637Mu02 氨基己糖苷酶Bβ(HEXβ)多克隆抗体 Polyclonal Antibody to Hexosaminidase B Beta (HEXb)

PAA637Hu02 氨基己糖苷酶Bβ(HEXβ)多克隆抗体 Polyclonal Antibody to Hexosaminidase B Beta (HEXb)

PAA195Hu01 氨基己糖苷酶Aα(HEXα)多克隆抗体 Polyclonal Antibody to Hexosaminidase A Alpha (HEXa)

PAA195Mu01 氨基己糖苷酶Aα(HEXα)多克隆抗体 Polyclonal Antibody to Hexosaminidase A Alpha (HEXa)

PAA195Mu02 氨基己糖苷酶Aα(HEXα)多克隆抗体 Polyclonal Antibody to Hexosaminidase A Alpha (HEXa)

PAB746Hu01 己糖激酶3(HK3)多克隆抗体 Polyclonal Antibody to Hexokinase 3, White Cell (HK3)

PAD352Hu01 己糖激酶2(HK2)多克隆抗体 Polyclonal Antibody to Hexokinase 2 (HK2)

PAD352Mu01 己糖激酶2(HK2)多克隆抗体 Polyclonal Antibody to Hexokinase 2 (HK2)

PAD352Hu02 己糖激酶2(HK2)多克隆抗体 Polyclonal Antibody to Hexokinase 2 (HK2)

PAA775Hu01 己糖激酶1(HK1)多克隆抗体 Polyclonal Antibody to Hexokinase 1 (HK1)

PAA775Mu01 己糖激酶1(HK1)多克隆抗体 Polyclonal Antibody to Hexokinase 1 (HK1)

PAA775Ra01 己糖激酶1(HK1)多克隆抗体 Polyclonal Antibody to Hexokinase 1 (HK1)

PAA775Hu02 己糖激酶1(HK1)多克隆抗体 Polyclonal Antibody to Hexokinase 1 (HK1)

PAA775Ra02 己糖激酶1(HK1)多克隆抗体 Polyclonal Antibody to Hexokinase 1 (HK1)

PAA775Mu02 己糖激酶1(HK1)多克隆抗体 Polyclonal Antibody to Hexokinase 1 (HK1)

PAA775Mu03 己糖激酶1(HK1)多克隆抗体 Polyclonal Antibody to Hexokinase 1 (HK1)

PAF128Hu01 不均一核核糖核蛋白U(HNRPU)多克隆抗体 Polyclonal Antibody to Heterogeneous Nuclear Ribonucleoprotein U (HNRPU)

PAF124Hu01 不均一核核糖核蛋白K(HNRPK)多克隆抗体 Polyclonal Antibody to Heterogeneous Nuclear Ribonucleoprotein K (HNRPK)

PAA323Hu01 不均一核糖核蛋白A2/B1(HNRPA2B1)多克隆抗体 Polyclonal Antibody to Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1)

PAC252Hu01 不均一核糖核蛋白A1(HNRPA1)多克隆抗体 Polyclonal Antibody to Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1)

PAC252Mu01 不均一核糖核蛋白A1(HNRPA1)多克隆抗体 Polyclonal Antibody to Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1)

PAN696Hu01 赫曼斯基普德拉克综合征蛋白6(HPS6)多克隆抗体 Polyclonal Antibody to Hermansky Pudlak Syndrome Protein 6 (HPS6)

PAH918Hu01 膜铁转运辅助蛋白(HEPH)多克隆抗体 Polyclonal Antibody to Hephaestin (HEPH)

PAB979Hu01 铁调素(Hepc)多克隆抗体 Polyclonal Antibody to Hepcidin (Hepc)

PAB979Mu01 铁调素(Hepc)多克隆抗体 Polyclonal Antibody to Hepcidin (Hepc)

PAB979Po01 铁调素(Hepc)多克隆抗体 Polyclonal Antibody to Hepcidin (Hepc)

PAB979Ra01 铁调素(Hepc)多克隆抗体 Polyclonal Antibody to Hepcidin (Hepc)

PAB979Bo01 铁调素(Hepc)多克隆抗体 Polyclonal Antibody to Hepcidin (Hepc)

PAB979Mu02 铁调素(Hepc)多克隆抗体 Polyclonal Antibody to Hepcidin (Hepc)

PAA624Hu01 肝癌源性生长因子(HDGF)多克隆抗体 Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)

PAA624Mu01 肝癌源性生长因子(HDGF)多克隆抗体 Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)

PAA624Ra01 肝癌源性生长因子(HDGF)多克隆抗体 Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)

PAA624Mu02 肝癌源性生长因子(HDGF)多克隆抗体 Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)

PAA418Hu01 肝细胞核因子4α(HNF4α)多克隆抗体 Polyclonal Antibody to Hepatocyte Nuclear Factor 4 Alpha (HNF4a)

PAG776Hu01 肝细胞核因子1β(HNF1β)多克隆抗体 Polyclonal Antibody to Hepatocyte Nuclear Factor 1 Beta (HNF1b)

PAG776Ra01 肝细胞核因子1β(HNF1β)多克隆抗体 Polyclonal Antibody to Hepatocyte Nuclear Factor 1 Beta (HNF1b)

PAG775Mu01 肝细胞核因子1α(HNF1α)多克隆抗体 Polyclonal Antibody to Hepatocyte Nuclear Factor 1 Alpha (HNF1a)

PAG775Mu02 肝细胞核因子1α(HNF1α)多克隆抗体 Polyclonal Antibody to Hepatocyte Nuclear Factor 1 Alpha (HNF1a)

PAG775Hu01 肝细胞核因子1α(HNF1α)多克隆抗体 Polyclonal Antibody to Hepatocyte Nuclear Factor 1 Alpha (HNF1a)

PAC186Hu01 肝细胞生长因子受体(HGFR)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor Receptor (HGFR)

PAH911Mu01 肝细胞生长因子激活因子(HGFAC)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor Activator (HGFAC)

PAH911Ra01 肝细胞生长因子激活因子(HGFAC)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor Activator (HGFAC)

PAH911Hu01 肝细胞生长因子激活因子(HGFAC)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor Activator (HGFAC)

PAA047Ca01 肝细胞生长因子(HGF)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

PAA047Hu01 肝细胞生长因子(HGF)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

PAA047Mu01 肝细胞生长因子(HGF)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

PAA047Ra01 肝细胞生长因子(HGF)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

PAA047Rb51 肝细胞生长因子(HGF)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

PAA047Hu02 肝细胞生长因子(HGF)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

PAA047Hu03 肝细胞生长因子(HGF)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

PAA047Bo01 肝细胞生长因子(HGF)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

PAA047Mu02 肝细胞生长因子(HGF)多克隆抗体 Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

PAN237Hu01 肝细胞癌相关蛋白1(HCRP1)多克隆抗体 Polyclonal Antibody to Hepatocellular Carcinoma Related Protein 1 (HCRP1)

PAH930Hu01 甲型肝炎病毒细胞受体2(HAVCR2)多克隆抗体 Polyclonal Antibody to Hepatitis A Virus Cellular Receptor 2 (HAVCR2)

PAA284Ga01 肝素辅因子Ⅱ(HCⅡ)多克隆抗体 Polyclonal Antibody to Heparin Cofactor II (HCII)

PAA284Hu01 肝素辅因子Ⅱ(HCⅡ)多克隆抗体 Polyclonal Antibody to Heparin Cofactor II (HCII)

PAA284Mu01 肝素辅因子Ⅱ(HCⅡ)多克隆抗体 Polyclonal Antibody to Heparin Cofactor II (HCII)

PAA284Ra01 肝素辅因子Ⅱ胰岛素抗体(HCⅡ)多克隆抗体 Polyclonal Antibody to Heparin Cofactor II (HCII)

PAA284Rb51 肝素辅因子Ⅱ(HCⅡ)多克隆抗体 Polyclonal Antibody to Heparin Cofactor II (HCII)

PAA284Mu02 肝素辅因子Ⅱ(HCⅡ)多克隆抗体 Polyclonal Antibody to Heparin Cofactor II (HCII)

PAB479Hu01 肝素结合性表皮生长因子(HBEGF)多克隆抗体 Polyclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)