诱导协同刺激分子配体CD275抗体
『别名』B7 H2; B7 homolog 2; B7 homologue 2; B7 like protein Gl50; B7 related protein 1; B7-like protein Gl50; B7-related protein 1; B7-H2; B7H2; B7RP 1; B7RP-1; B7RP1; CD 275; CD275; CD275 antigen; GL 50; GL50; ICOS L; ICOS LG; ICOS ligand; ICOSL; ICOSL_HUMAN; Icoslg; Inducible T cell co stimulator ligand; KIAA0653; LICOS; Transmembrane protein B7 H2 ICOS ligand; ICOSLG.
『浓度』诱导协同刺激分子配体CD275抗体1mg/1ml
『规格』0.1ml/100μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
诱导协同刺激分子配体CD275抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
诱导协同刺激分子配体CD275抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAC563Mu02 白细胞衍生趋化因子1(LECT1)多克隆抗体 Polyclonal Antibody to Leukocyte Cell Derived Chemotaxin 1 (LECT1)
PAB000Hu01 白细胞关联免疫球蛋白样受体2(LAIR2)多克隆抗体 Polyclonal Antibody to Leukocyte Associated Immunoglobulin Like Receptor 2 (LAIR2)
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PAA561Mu01 白血病抑制因子受体(LIFR)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor Receptor (LIFR)
PAA561Ra01 白血病抑制因子受体(LIFR)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor Receptor (LIFR)
PAA561Hu02 白血病抑制因子受体(LIFR)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor Receptor (LIFR)
PAA561Hu01 白血病抑制因子受体(LIFR)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor Receptor (LIFR)
PAA561Mu03 白血病抑制因子受体(LIFR)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor Receptor (LIFR)
PAA561Hu03 白血病抑制因子受体(LIFR)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor Receptor (LIFR)
PAA561Mu02 白血病抑制因子受体(LIFR)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor Receptor (LIFR)
PAA561Ra02 白血病抑制因子受体(LIFR)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor Receptor (LIFR)
PAA561Ra03 白血病抑制因子受体(LIFR)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor Receptor (LIFR)
PAA085Bo01 白血病抑制因子(LIF)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)
PAA085Ca01 白血病抑制因子(LIF)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)
PAA085Cp01 白血病抑制因子(LIF)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)
PAA085Hu01 白血病抑制因子(LIF)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)
PAA085Mu01 白血病抑制因子(LIF)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)
PAA085Po01 白血病抑制因子(LIF)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)
PAA085Ra01 白血病抑制因子(LIF)多克隆抗体 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)
PAH723Hu01 亮氨酰/半胱氨酰氨肽酶(LNPEP)多克隆抗体 Polyclonal Antibody to Leucyl/Cystinyl Aminopeptidase (LNPEP)
PAM770Mu01 亮氨酸丰富重复免疫球蛋白样域蛋白3(LRIG3)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeats And Immunoglobulin Like Domains Protein 3 (LRIG3)
PAL673Hu01 亮氨酸丰富重复免疫球蛋白样域蛋白1(LRIG1)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeats And Immunoglobulin Like Domains Protein 1 (LRIG1)
PAL681Hu01 亮氨酸丰富重复神经元蛋白1(LRRN1)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Neuronal Protein 1 (LRRN1)
PAK166Hu01 亮氨酸丰富重复LGI家族成员3(LGI3)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat LGI Family, Member 3 (LGI3)
PAK166Mu01 亮氨酸丰富重复LGI家族成员3(LGI3)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat LGI Family, Member 3 (LGI3)
PAK166Mu02 亮氨酸丰富重复LGI家族成员3(LGI3)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat LGI Family, Member 3 (LGI3)
PAH716Hu01 亮氨酸丰富重复激酶2(LRRK2)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Kinase 2 (LRRK2)
PAH716Hu02 亮氨酸丰富重复激酶2(LRRK2)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Kinase 2 (LRRK2)
PAH716Mu01 亮氨酸丰富重复激酶2(LRRK2)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Kinase 2 (LRRK2)
PAH716Ra01 亮氨酸丰富重复激酶2(LRRK2)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Kinase 2 (LRRK2)
PAH718Hu01 亮氨酸丰富重复FLII相互作用蛋白1(LRRFIP1)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1)
PAH718Hu02 亮氨酸丰富重复FLII相互作用蛋白1(LRRFIP1)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1)
PAS728Hu01 含亮氨酸丰富重复蛋白4B(LRRC4B)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Containing Protein 4B (LRRC4B)
PAW764Hu01 含亮氨酸丰富重复蛋白3C(LRRC3C)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Containing Protein 3C (LRRC3C)
PAW764Mu01 含亮氨酸丰富重复蛋白3C(LRRC3C)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Containing Protein 3C (LRRC3C)
PAW764Mu02 含亮氨酸丰富重复蛋白3C(LRRC3C)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Containing Protein 3C (LRRC3C)
PAQ040Mu01 含亮氨酸丰富重复蛋白32(LRRC32)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Containing Protein 32 (LRRC32)
PAQ040Ra01 含亮氨酸丰富重复蛋白32(LRRC32)多克隆抗体 Polyclonal Antibody to Leucine Rich Repeat Containing Protein 32 (LRRC32)
PAB934Hu01 亮氨酸丰富α2-糖蛋白1(LRG1)多克隆抗体 Polyclonal Antibody to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)
PAB934Mu01 亮氨酸丰富α2-糖蛋白1(LRG1)多克隆抗体 Polyclonal Antibody to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)
PAB934Ra01 亮氨酸丰富α2-糖蛋白1(LRG1)多克隆抗体 Polyclonal Antibody to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)
PAB934Mu02 亮氨酸丰富α2-糖蛋白1(LRG1)多克隆抗体 Polyclonal Antibody to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)
PAB934Ra02 亮氨酸丰富α2-糖蛋白1(LRG1)多克隆抗体 Polyclonal Antibody to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)
PAA536Hu01 亮氨酰氨基肽酶(LAP)多克隆抗体 Polyclonal Antibody to Leucine Aminopeptidase (LAP)
PAA536Ra01 亮氨酰氨基肽酶(LAP)多克隆抗体 Polyclonal Antibody to Leucine Aminopeptidase (LAP)
PAS110Ge01 亮氨酸(Leu)多克隆抗体 Polyclonal Antibody to Leucine (Leu)
PAR202Hu01 含LETM1域蛋白1(LETMD1)多克隆抗体 Polyclonal Antibody to LETM1 Domain Containing Protein 1 (LETMD1)
PAA083Hu01 瘦素受体(LEPR)多克隆抗体 Polyclonal Antibody to Leptin Receptor (LEPR)
PAA083Mu01 瘦素受体(LEPR)多克隆抗体 Polyclonal Antibody to Leptin Receptor (LEPR)
PAA083Po01 瘦素受体(LEPR)多克隆抗体 Polyclonal Antibody to Leptin Receptor (LEPR)
PAA083Ra01 瘦素受体(LEPR)多克隆抗体 Polyclonal Antibody to Leptin Receptor (LEPR)
PAA083Mu02 瘦素受体(LEPR)多克隆抗体 Polyclonal Antibody to Leptin Receptor (LEPR)
PAA084Mu01 瘦素(LEP)多克隆抗体 Polyclonal Antibody to Leptin (LEP)
PAA084Ra01 瘦素(LEP)多克隆抗体 Polyclonal Antibody to Leptin (LEP)
PAA084Rb51 瘦素诱导协同刺激分子配体CD275抗体(LEP)多克隆抗体 Polyclonal Antibody to Leptin (LEP)
PAA084Ca01 瘦素(LEP)多克隆抗体 Polyclonal Antibody to Leptin (LEP)
PAA084Po01 瘦素(LEP)多克隆抗体 Polyclonal Antibody to Leptin (LEP)
PAA084Bo01 瘦素(LEP)多克隆抗体 Polyclonal Antibody to Leptin (LEP)
PAA084Cp01 瘦素(LEP)多克隆抗体 Polyclonal Antibody to Leptin (LEP)