兔可溶性CD40配体检测试剂盒折扣
我们免费代测,品牌供应,我们拥有三千多种酶联免疫吸附测定试剂盒,包含多种种属ELISA试剂盒,待测标本形式多样:有液体及半固体形式。
样本保存:
2-8℃冰箱最多2周
-20到-50℃的冰箱可以放3-6个月
兔可溶性CD40配体检测试剂盒折扣-50℃一下的冰箱,可以放一年
样本收集:
血清样本用干燥管(红色)或者促凝管(黄色)收集
血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
血浆样本最好是不要用肝素(绿色)抗凝的试管收集
样本处理:
1)血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2)血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3)尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4)细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。兔可溶性CD40配体检测试剂盒折扣通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5)组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6)标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7)不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
EIA(B004)-000225 大鼠70kDa热休克蛋白4(HSPA4)ELISA Kit ,Products name: Rat HSPA4 (Heat Shock 70kDa Protein 4) ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-000227 大鼠70kDa热休克蛋白5(HSPA5)ELISA Kit ,Products name: HSPA5 ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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EIA(B004)-000232 大鼠8羟基脱氧鸟苷(8-OHdG)ELISA Kit ,Products name: Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-000233 大鼠8羟基脱氧鸟苷(8-OHdG)ELISA Kit ,Products name: 8-OHdG ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-000234 大鼠8羟基脱氧鸟苷(8-OHdG)ELISA Kit ,Products name: 8-OHdG ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-000235 大鼠8-羟基脱氧鸟苷(8-OHdG)ELISA Kit ,Products name: 8-OHdG ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
EIA(B004)-000236 大鼠8-异构前列腺素F2α(8-epi-PGF2α)ELISA Kit ,Products name: 8-epi-PGF2α ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-000237 大鼠8异前列腺素(8-iso-PG)ELISA Kit ,Products name: Rat 8-iso prostaglandin,8-iso-PG ELISA Kit ,灵敏度: 1.875ng/mL ,检测范围: 3.125-200ng/mL
EIA(B004)-000238 大鼠8异前列腺素(8-iso-PG)ELISA Kit ,Products name: Rat 8-iso prostaglandin,8-iso-PG ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B004)-000239 大鼠8异前列腺素(8-iso-PG)ELISA Kit ,Products name: 8-iso-PG ELISA Kit ,灵敏度: 9.375pg/mL ,检测范围: 15.625-1000pg/mL
EIA(B004)-000240 大鼠8异前列腺素(8-iso-PG)ELISA Kit ,Products name: 8-iso-PG ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
EIA(B004)-000241 大鼠8异前列腺素2a(8isoPG2a)ELISA Kit ,Products name: 8isoPG2a ELISA Kit ,灵敏度: 0.93799999999999994ng/mL ,检测范围: 1.5629999999999999-100ng/mL
EIA(B004)-000242 大鼠8异前列腺素F2a(8isoPGF2a)ELISA Kit ,Products name: 8isoPGF2a ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-000243 大鼠8异前列腺素F2α(8-iso-PGF2a)ELISA Kit ,Products name: 8-iso-PGF2a ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
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EIA(B004)-000245 大鼠90kDa热休克蛋白αB1(HSP90αB1)ELISA Kit ,Products name: HSP90αB1 ELISA Kit ,灵敏度: 46.875pg/mL ,检测范围: 78.125-5000pg/mL
EIA(B004)-000246 大鼠90kDa热休克蛋白β1(HSP90β1)ELISA Kit ,Products name: HSP90β1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-000248 大鼠Ⅰ型胶原(COL1)ELISA Kit ,Products name: COL1 ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B004)-000249 大鼠Ⅰ型胶原(ColⅠ)ELISA Kit ,Products name: ColⅠ ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
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EIA(B004)-000255 大鼠Ⅰ型胶原C端肽(CTX-Ⅰ)ELISA Kit ,Products name: CTX-Ⅰ ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-000257 大鼠Ⅰ型胶原N末端肽(NTX)ELISA Kit ,Products name: Rat cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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EIA(B004)-000260 大鼠Ⅰ型胶原N末端肽(NTX)ELISA Kit ,Products name: NTX ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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EIA(B004)-000276 大鼠Ⅰ型前胶原N端前肽(PⅠNP)ELISA Kit ,Products name: Rat procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-000278 大鼠Ⅰ型前胶原N端前肽(PⅠNP)ELISA Kit ,Products name: PⅠNP ELISA Kit ,灵敏度: 9.375pg/ml ,检测范围: 15.625-1000pg/ml
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