兔热休克蛋白70检测试剂盒折扣
我们免费代测,品牌供应,我们拥有三千多种酶联免疫吸附测定试剂盒,包含多种种属ELISA试剂盒,待测标本形式多样:有液体及半固体形式。
样本保存:
2-8℃冰箱最多2周
-20到-50℃的冰箱可以放3-6个月
兔热休克蛋白70检测试剂盒折扣-50℃一下的冰箱,可以放一年
样本收集:
血清样本用干燥管(红色)或者促凝管(黄色)收集
血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
血浆样本最好是不要用肝素(绿色)抗凝的试管收集
样本处理:
1)血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2)血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3)尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4)细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。兔热休克蛋白70检测试剂盒折扣通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5)组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6)标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7)不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
EIA(B003)-051321 猪胃泌素(GAS)ELISA检测试剂盒 ,Products name: GAS ELISA Kit ,灵敏度: 75pg/mL ,检测范围: 125-8000pg/mL
EIA(B003)-051322 猪胃泌素(GT)ELISA检测试剂盒 ,Products name: GT ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
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EIA(B003)-051325 猪胃泌素抑制肽(GIP)ELISA检测试剂盒 ,Products name: GIP ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
EIA(B003)-051326 猪胃泌酸调节素(OXM)ELISA检测试剂盒 ,Products name: OXM ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B003)-051327 猪瘟抗体(CSFV)ELISA检测试剂盒 ,Products name: CSFV ELISA Kit ,灵敏度: 0.375ng/mL ,检测范围: 0.625-40ng/mL
EIA(B003)-051328 猪乌头酸酶2(ACO-2)ELISA检测试剂盒 ,Products name: ACO-2 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B003)-051329 猪无孢蛋白(ASPN)ELISA检测试剂盒 ,Products name: ASPN ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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EIA(B003)-051331 猪戊糖素(PE)ELISA检测试剂盒 ,Products name: PE ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
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EIA(B003)-051333 猪硒蛋白P(SEPP1)ELISA检测试剂盒 ,Products name: SEPP1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B003)-051334 猪系统性红斑狼疮(SLE)ELISA检测试剂盒 ,Products name: SLE ELISA Kit ,灵敏度: 9.375pg/mL ,检测范围: 15.625-1000pg/mL
EIA(B003)-051335 猪细胞(JNK/SAPK)蛋白表达比色法ELISA检测试剂盒 ,Products name: JNK/SAPK ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
EIA(B003)-051336 猪细胞凋亡关联酪氨酸激酶(AATK)ELISA检测试剂盒 ,Products name: AATK ELISA Kit ,灵敏度: 0.75ng/mL ,检测范围: 1.25-80ng/mL
EIA(B003)-051337 猪细胞凋亡抑制因子(IAP)ELISA检测试剂盒 ,Products name: IAP ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B003)-051338 猪细胞毒T淋巴细胞相关抗原4(CTLA4)ELISA检测试剂盒 ,Products name: CTLA4 ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B003)-051339 猪细胞分裂周期蛋白23(CDC23)ELISA检测试剂盒 ,Products name: CDC23 ELISA Kit ,灵敏度: 37.5pg/mL ,检测范围: 62.5-4000pg/mL
EIA(B003)-051340 猪细胞分裂周期基因(CDC)ELISA检测试剂盒 ,Products name: CDC ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
EIA(B003)-051341 猪细胞附着蛋白1(CYTH1)ELISA检测试剂盒 ,Products name: CYTH1 ELISA Kit ,灵敏度: 0.93799999999999994ng/mL ,检测范围: 1.5629999999999999-100ng/mL
EIA(B003)-051342 猪细胞胱硫醚γ裂解酶(CSE)ELISA检测试剂盒 ,Products name: CSE ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B003)-051343 猪细胞间黏附分子1(ICAM-1)ELISA检测试剂盒 ,Products name: ICAM-1 ELISA Kit ,灵敏度: 37.5pg/mL ,检测范围: 62.5-4000pg/mL
EIA(B003)-051344 猪细胞间粘附分子1(ICAM1)ELISA检测试剂盒 ,Products name: ICAM1 ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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