胰岛素样生长因子I受体抗体
『别名』CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF I receptor; IGF1R; IGFIR; IGFIRC; Insulin like growth factor 1 receptor; Insulin like growth factor 1 receptor precursor; JTK13; MGC142170; MGC142172; IGF1R_HUMAN.
『浓度』胰岛素样生长因子I受体抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
胰岛素样生长因子I受体抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
胰岛素样生长因子I受体抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
ELB1169c 兔软骨糖蛋白39(GP39)酶联免疫吸附测定试剂盒 Rabbit GP39 (Glycoprotein 39, Cartilage) ELISA Kit
ELB0084c 兔抗糖蛋白抗体(GP)酶联免疫吸附测定试剂盒 Rabbit GP (Anti-Glycoprotein Antibody) ELISA Kit
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ELB1558c 兔粒细胞巨噬细胞集落刺激因子抗体(GM-CSF Ab)酶联免疫吸附测定试剂盒 Rabbit GM-CSF Ab (Anti-Granulocyte Macrophage Colony Stimulating Factor Antibody) ELISA Kit
ELB0865c 兔胃粘液素(GM)酶联免疫吸附测定试剂盒 Rabbit GM (Gastric Mucin) ELISA Kit
ELB1770c 兔半乳糖苷酶β(GLβ)酶联免疫吸附测定试剂盒 Rabbit GLβ (Galactosidase Beta) ELISA Kit
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ELB0235c 兔糖基化依赖的细胞黏附分子(GlyCAM1)酶联免疫吸附测定试剂盒 Rabbit GlyCAM1 (Glycosylation Dependent Cell Adhesion Molecule 1) ELISA Kit
ELB1361c 兔葡萄糖转运蛋白4(GLUT4)酶联免疫吸附测定试剂盒 Rabbit GLUT4 (Glucose Transporter 4) ELISA Kit
ELB1701c 兔葡萄糖转运蛋白3(GLUT3)酶联免疫吸附测定试剂盒 Rabbit GLUT3 (Glucose Transporter 3) ELISA Kit
ELB1886c 兔葡萄糖转运蛋白2(GLUT2)酶联免疫吸附测定试剂盒 Rabbit GLUT2 (Glucose Transporter 2) ELISA Kit
ELB1424c 兔葡萄糖转运蛋白1(GLUT1)酶联免疫吸附测定试剂盒 Rabbit GLUT1 (Glucose Transporter 1) ELISA Kit
ELB1207c 兔谷氧还蛋白3(GLRX3)酶联免疫吸附测定试剂盒 Rabbit GLRX3 (Glutaredoxin 3) ELISA Kit
ELB0460c 兔胰高血糖素样肽2(GLP-2)酶联免疫吸附测定试剂盒 Rabbit GLP-2 (Glucagon Like Peptide 2) ELISA Kit
ELB0021c 兔胰高血糖素样肽1(GLP-1)酶联免疫吸附测定试剂盒 Rabbit GLP-1(Glucagon Like Peptide 1)ELISA Kit
ELB0752c 兔葡萄糖激酶调节蛋白(GKRP)酶联免疫吸附测定试剂盒 Rabbit GKRP (Glucokinase Regulatory Protein) ELISA Kit
ELB0508c 兔胃动蛋白2(GKN2)酶联免疫吸附测定试剂盒 Rabbit GKN2 (Gastrokine 2) ELISA Kit
ELB1564c 兔胃动蛋白1(GKN1)酶联免疫吸附测定试剂盒 Rabbit GKN1 (Gastrokine 1) ELISA Kit
ELB1130c 兔间隙连接蛋白β1(GJβ1)酶联免疫吸附测定试剂盒 Rabbit GJβ1 (Gap Junction Protein Beta 1) ELISA Kit
ELB0823c 兔胃泌素抑制肽(GIP)酶联免疫吸附测定试剂盒 Rabbit GIP (Gastric Inhibitory Polypeptide) ELISA Kit
ELB1635c 兔胃内因子(GIF)酶联免疫吸附测定试剂盒 Rabbit GIF (Gastric Intrinsic Factor) ELISA Kit
ELB1055c 兔促生长激素释放激素(GHRH)酶联免疫吸附测定试剂盒 Rabbit GHRH (Growth Hormone Releasing Hormone) ELISA Kit
ELB2158c 兔生长激素受体(GHR)酶联免疫吸附测定试剂盒 Rabbit GHR (Growth Hormone Receptor) ELISA Kit
ELB2014c 兔生长激素诱导跨膜蛋白(GHITM)酶联免疫吸附测定试剂盒 Rabbit GHITM (Growth Hormone Inducible Transmembrane Protein) ELISA Kit
ELB0173c 兔抗生长激素抗体(GHAb)酶联免疫吸附测定试剂盒 Rabbit GHAb (Anti-Growth Hormone Antibody) ELISA Kit
ELB2165c 兔生长激素2(GH2)酶联免疫吸附测定试剂盒 Rabbit GH2 (Growth Hormone 2) ELISA Kit
ELB2030c 兔促生长激素(GH)酶联免疫吸附测定试剂盒 Rabbit GH (Growth Hormone) ELISA Kit
ELB1068c 兔神经胶质纤维酸性蛋白(GFAP)酶联免疫吸附测定试剂盒 Rabbit GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
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ELB0707c 兔生长分化因子3(GDF3)酶联免疫吸附测定试剂盒 Rabbit GDF3 (Growth Differentiation Factor 3) ELISA Kit
ELB0991c 兔生长分化因子2(GDF2)酶联免疫吸附测定试剂盒 Rabbit GDF2 (Growth Differentiation Factor 2) ELISA Kit
ELB0129c 兔生长分化因子15(GDF15)酶联免疫吸附测定试剂盒 Rabbit GDF15 (Growth Differentiation Factor 15) ELISA Kit
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ELB2036c 兔GATA结合蛋白1(GATA1)酶联免疫吸附测定试剂盒 Rabbit GATA1 (GATA Binding Protein 1) ELISA Kit
ELB0434c 兔促胃液素受体(GasR)酶联免疫吸附测定试剂盒 Rabbit GasR (Gastrin Receptor) ELISA Kit